Detailed Information on Publication Record
2020
A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response
DUMKOVÁ, Jana, Tereza SMUTNÁ, Lucie VRLÍKOVÁ, Bohumil DOČEKAL, Daniela KRISTEKOVÁ et. al.Basic information
Original name
A Clearance Period after Soluble Lead Nanoparticle Inhalation Did Not Ameliorate the Negative Effects on Target Tissues Due to Decreased Immune Response
Authors
DUMKOVÁ, Jana (203 Czech Republic, belonging to the institution), Tereza SMUTNÁ (203 Czech Republic, belonging to the institution), Lucie VRLÍKOVÁ (203 Czech Republic), Bohumil DOČEKAL (203 Czech Republic), Daniela KRISTEKOVÁ (703 Slovakia, belonging to the institution), Zbyněk VEČEŘA (203 Czech Republic), Zuzana HUSÁKOVÁ (203 Czech Republic, belonging to the institution), Veronika JAKEŠOVÁ (203 Czech Republic), Adriena JEDLIČKOVÁ (203 Czech Republic), Pavel MIKUŠKA (203 Czech Republic), Lukáš ALEXA (203 Czech Republic), Pavel COUFALÍK (203 Czech Republic), Michaela TVRDOŇOVÁ (203 Czech Republic, belonging to the institution), Kamil KŘŮMAL (203 Czech Republic), Tomáš VACULOVIČ (203 Czech Republic, belonging to the institution), Viktor KANICKÝ (203 Czech Republic, belonging to the institution), Aleš HAMPL (203 Czech Republic, belonging to the institution) and Marcela BUCHTOVÁ (203 Czech Republic, guarantor, belonging to the institution)
Edition
International Journal of Molecular Sciences, Basel, MDPI, 2020, 1422-0067
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30108 Toxicology
Country of publisher
Switzerland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 5.923
RIV identification code
RIV/00216224:14310/20:00114496
Organization unit
Faculty of Science
UT WoS
000594717000001
Keywords in English
clearance; lead nanoparticles; inhalation; LA-ICP-MS imaging; toxicity
Tags
International impact, Reviewed
Změněno: 17/2/2023 12:12, Mgr. Marie Šípková, DiS.
Abstract
V originále
The inhalation of metal (including lead) nanoparticles poses a real health issue to people and animals living in polluted and/or industrial areas. In this study, we exposed mice to lead(II) nitrate nanoparticles [Pb(NO3)(2) NPs], which represent a highly soluble form of lead, by inhalation. We aimed to uncover the effects of their exposure on individual target organs and to reveal potential variability in the lead clearance. We examined (i) lead biodistribution in target organs using laser ablation and inductively coupled plasma mass spectrometry (LA-ICP-MS) and atomic absorption spectrometry (AAS), (ii) lead effect on histopathological changes and immune cells response in secondary target organs and (iii) the clearance ability of target organs. In the lungs and liver, Pb(NO3)(2) NP inhalation induced serious structural changes and their damage was present even after a 5-week clearance period despite the lead having been almost completely eliminated from the tissues. The numbers of macrophages significantly decreased after 11-week Pb(NO3)(2) NP inhalation; conversely, abundance of alpha-smooth muscle actin (alpha-SMA)-positive cells, which are responsible for augmented collagen production, increased in both tissues. Moreover, the expression of nuclear factor kappa B (NF-kappa B) and selected cytokines, such as tumor necrosis factor alpha (TNF alpha), transforming growth factor beta 1 (TGF beta 1), interleukin 6(IL-6), IL-1 alpha and IL-1 beta , displayed a tissue-specific response to lead exposure. In summary, diminished inflammatory response in tissues after Pb(NO3)(2) NPs inhalation was associated with prolonged negative effect of lead on tissues, as demonstrated by sustained pathological changes in target organs, even after long clearance period.
Links
| GA20-02203S, research and development project |
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