J 2020

Uveal melanoma - testing of abnormalities of chromosome 3 and 8 in the Czech Republic

MATUŠKOVÁ, Veronika, Pavla HORŇÁČKOVÁ, Radoslava UHMANNOVÁ, Lenka ZLÁMALÍKOVÁ, Květoslava LIŠKOVÁ et. al.

Basic information

Original name

Uveal melanoma - testing of abnormalities of chromosome 3 and 8 in the Czech Republic

Authors

MATUŠKOVÁ, Veronika (203 Czech Republic, guarantor, belonging to the institution), Pavla HORŇÁČKOVÁ (203 Czech Republic, belonging to the institution), Radoslava UHMANNOVÁ (203 Czech Republic), Lenka ZLÁMALÍKOVÁ (203 Czech Republic, belonging to the institution), Květoslava LIŠKOVÁ (203 Czech Republic, belonging to the institution), Michal UHER (203 Czech Republic, belonging to the institution) and Eva VLKOVÁ (203 Czech Republic, belonging to the institution)

Edition

Biomedical Papers, Olomouc, Univerzita Palackého v Olomouci, 2020, 1213-8118

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30207 Ophthalmology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 1.245

RIV identification code

RIV/00216224:14110/20:00117059

Organization unit

Faculty of Medicine

UT WoS

000604951200008

Keywords in English

uveal melanoma; chromosome 3; chromosome 8; CISH; FISH; progression-free survival

Tags

International impact, Reviewed
Změněno: 27/1/2021 09:55, Mgr. Tereza Miškechová

Abstract

V originále

Aim: The aim of this cytogenetic study is to confirm the significance of chromosome 3 loss (monosomy 3) and of the gain of chromosome 8 as prognostic markers in histopathological samples of enucleated eyes with uveal melanoma in the Czech population. Methods: This is a retrospective study of 52 enucleated eyes. Chromosome 3 and 8 status were tested by CISH, and in a few samples FISH was used. The correlation between monosomy 3 and gain of chromosome 8 and clinical features (histopathological type, size of the tumour) were evaluated. A follow up for the detection of metastases was conducted in all patients. The statistical significance of chromosomal abnormalities as a prognostic factor for the development of metastases was calculated. Results: There were 52 patients, 27 men (51.9%) and 25 women (48.1%) enrolled in our study group. The mean age was 63 ± 14 years. Loss of the one copy of chromosome 3 (monosomy 3) was detected in 26 (50.0%) patients, monosomy 8 was present in 34.6% of patients with monosomy 3. After 5 years there were no metastases in 82% of patients without monosomy 3 as opposed to 40% of patients with monosomy 3. We confirmed a statistically significant association between progression free survival (PFS) and the presence of monosomy 3 (P=0.017). The association between PFS and gain of chromosome 8 was significant as well (0.010). Conclusions: Our data showed the association of progression-free survival with the presence of monosomy 3 in uveal melanomas. We provided a good prognostic value of monosomy 3 in uveal melanoma.