Developmental mechanisms driving complex tooth shape in
reptiles

J 2020

Developmental mechanisms driving complex tooth shape in reptiles

ŠULCOVÁ, Marie, Oldrich ZAHRADNICEK, Jana DUMKOVÁ, Hana DOSEDELOVA, Jan KŘIVÁNEK et. al.

Basic information

Original name

Developmental mechanisms driving complex tooth shape in reptiles

Authors

ŠULCOVÁ, Marie (203 Czech Republic, belonging to the institution), Oldrich ZAHRADNICEK, Jana DUMKOVÁ (203 Czech Republic, belonging to the institution), Hana DOSEDELOVA, Jan KŘIVÁNEK (203 Czech Republic, belonging to the institution), Marek HAMPL (203 Czech Republic, belonging to the institution), Michaela KAVKOVA, Tomas ZIKMUND, Martina GREGOROVICOVA, David SEDMERA, Jozef KAISER, Abigail S. TUCKER and Marcela BUCHTOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Developmental dynamics, Hoboken, Wiley, 2020, 1058-8388

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10602 Biology , Evolutionary biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.780

RIV identification code

RIV/00216224:14310/20:00114512

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/dvdy.138

UT WoS

000502529500001

Keywords in English

chameleon; crocodile; enamel ridge; gecko; matriptase; Na; K-ATPase; nuclei shape; SHH; tooth shape

Abstract

V originále

Background In mammals, odontogenesis is regulated by transient signaling centers known as enamel knots (EKs), which drive the dental epithelium shaping. However, the developmental mechanisms contributing to formation of complex tooth shape in reptiles are not fully understood. Here, we aim to elucidate whether signaling organizers similar to EKs appear during reptilian odontogenesis and how enamel ridges are formed. Results Morphological structures resembling the mammalian EK were found during reptile odontogenesis. Similar to mammalian primary EKs, they exhibit the presence of apoptotic cells and no proliferating cells. Moreover, expression of mammalian EK-specific molecules (SHH, FGF4, and ST14) and GLI2-negative cells were found in reptilian EK-like areas. 3D analysis of the nucleus shape revealed distinct rearrangement of the cells associated with enamel groove formation. This process was associated with ultrastructural changes and lipid droplet accumulation in the cells directly above the forming ridge, accompanied by alteration of membranous molecule expression (Na/K-ATPase) and cytoskeletal rearrangement (F-actin). Conclusions The final complex shape of reptilian teeth is orchestrated by a combination of changes in cell signaling, cell shape, and cell rearrangement. All these factors contribute to asymmetry in the inner enamel epithelium development, enamel deposition, ultimately leading to the formation of characteristic enamel ridges.

Links

GA17-14886S, research and development project

Name: Molekulární a buněčná dynamika rozhraní zubu a kosti u modelových druhů s akrodontní, pleurodontní a tekodontní denticí

Investor: Czech Science Foundation

LM2015062, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

ŠULCOVÁ, Marie, Oldrich ZAHRADNICEK, Jana DUMKOVÁ, Hana DOSEDELOVA, Jan KŘIVÁNEK, Marek HAMPL, Michaela KAVKOVA, Tomas ZIKMUND, Martina GREGOROVICOVA, David SEDMERA, Jozef KAISER, Abigail S. TUCKER and Marcela BUCHTOVÁ. Developmental mechanisms driving complex tooth shape in reptiles. Developmental dynamics. Hoboken: Wiley, 2020, vol. 249, No 4, p. 441-464. ISSN 1058-8388. Available from: https://dx.doi.org/10.1002/dvdy.138.

@article{1699600,
   author = {Šulcová, Marie and Zahradnicek, Oldrich and Dumková, Jana and Dosedelova, Hana and Křivánek, Jan and Hampl, Marek and Kavkova, Michaela and Zikmund, Tomas and Gregorovicova, Martina and Sedmera, David and Kaiser, Jozef and Tucker, Abigail S. and Buchtová, Marcela},
   article_location = {Hoboken},
   article_number = {4},
   doi = {http://dx.doi.org/10.1002/dvdy.138},
   keywords = {chameleon; crocodile; enamel ridge; gecko; matriptase; Na; K-ATPase; nuclei shape; SHH; tooth shape},
   language = {eng},
   issn = {1058-8388},
   journal = {Developmental dynamics},
   title = {Developmental mechanisms driving complex tooth shape in reptiles},
   url = {https://doi.org/10.1002/dvdy.138},
   volume = {249},
   year = {2020}
}

TY  - JOUR
ID  - 1699600
AU  - Šulcová, Marie - Zahradnicek, Oldrich - Dumková, Jana - Dosedelova, Hana - Křivánek, Jan - Hampl, Marek - Kavkova, Michaela - Zikmund, Tomas - Gregorovicova, Martina - Sedmera, David - Kaiser, Jozef - Tucker, Abigail S. - Buchtová, Marcela
PY  - 2020
TI  - Developmental mechanisms driving complex tooth shape in reptiles
JF  - Developmental dynamics
VL  - 249
IS  - 4
SP  - 441-464
EP  - 441-464
PB  - Wiley
SN  - 10588388
KW  - chameleon
KW  - crocodile
KW  - enamel ridge
KW  - gecko
KW  - matriptase
KW  - Na
KW  - K-ATPase
KW  - nuclei shape
KW  - SHH
KW  - tooth shape
UR  - https://doi.org/10.1002/dvdy.138
L2  - https://doi.org/10.1002/dvdy.138
N2  - Background In mammals, odontogenesis is regulated by transient signaling centers known as enamel knots (EKs), which drive the dental epithelium shaping. However, the developmental mechanisms contributing to formation of complex tooth shape in reptiles are not fully understood. Here, we aim to elucidate whether signaling organizers similar to EKs appear during reptilian odontogenesis and how enamel ridges are formed. Results Morphological structures resembling the mammalian EK were found during reptile odontogenesis. Similar to mammalian primary EKs, they exhibit the presence of apoptotic cells and no proliferating cells. Moreover, expression of mammalian EK-specific molecules (SHH, FGF4, and ST14) and GLI2-negative cells were found in reptilian EK-like areas. 3D analysis of the nucleus shape revealed distinct rearrangement of the cells associated with enamel groove formation. This process was associated with ultrastructural changes and lipid droplet accumulation in the cells directly above the forming ridge, accompanied by alteration of membranous molecule expression (Na/K-ATPase) and cytoskeletal rearrangement (F-actin). Conclusions The final complex shape of reptilian teeth is orchestrated by a combination of changes in cell signaling, cell shape, and cell rearrangement. All these factors contribute to asymmetry in the inner enamel epithelium development, enamel deposition, ultimately leading to the formation of characteristic enamel ridges.
ER  -

ŠULCOVÁ, Marie, Oldrich ZAHRADNICEK, Jana DUMKOVÁ, Hana DOSEDELOVA, Jan KŘIVÁNEK, Marek HAMPL, Michaela KAVKOVA, Tomas ZIKMUND, Martina GREGOROVICOVA, David SEDMERA, Jozef KAISER, Abigail S. TUCKER and Marcela BUCHTOVÁ. Developmental mechanisms driving complex tooth shape in reptiles. \textit{Developmental dynamics}. Hoboken: Wiley, 2020, vol.~249, No~4, p.~441-464. ISSN~1058-8388. Available from: https://dx.doi.org/10.1002/dvdy.138.

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