12-O-Tetradecanoylphor bol-13-acetate increases
cardiomyogenesis through PKC/ERK signaling

J 2020

12-O-Tetradecanoylphor bol-13-acetate increases cardiomyogenesis through PKC/ERK signaling

RADASZKIEWICZ, Katarzyna Anna, Deborah BECKEROVÁ, Lucie WOLOSZCZUKOVÁ, Tomasz Witold RADASZKIEWICZ, Petra LESÁKOVÁ et. al.

Basic information

Original name

12-O-Tetradecanoylphor bol-13-acetate increases cardiomyogenesis through PKC/ERK signaling

Authors

RADASZKIEWICZ, Katarzyna Anna (616 Poland, belonging to the institution), Deborah BECKEROVÁ (203 Czech Republic, belonging to the institution), Lucie WOLOSZCZUKOVÁ (203 Czech Republic, belonging to the institution), Tomasz Witold RADASZKIEWICZ (616 Poland, belonging to the institution), Petra LESÁKOVÁ (203 Czech Republic, belonging to the institution), Olga VONDÁLOVÁ BLANÁŘOVÁ (203 Czech Republic, belonging to the institution), Lukáš KUBALA (203 Czech Republic, belonging to the institution), Petr HUMPOLÍČEK and Jiří PACHERNÍK (203 Czech Republic, guarantor, belonging to the institution)

Edition

Scientific reports, Berlin, Nature Research, 2020, 2045-2322

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.379

RIV identification code

RIV/00216224:14310/20:00114532

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1038/s41598-020-73074-4

UT WoS

000577348000016

Keywords in English

Cell biology; Developmental biology; Stem cells

Abstract

V originále

12-O-Tetradecanoylphorbol-13-acetate (TPA) is the most widely used diacylglycerol (DAG) mimetic agent and inducer of protein kinase C (PKC)-mediated cellular response in biomedical studies. TPA has been proposed as a pluripotent cell differentiation factor, but results obtained have been inconsistent. In the present study we show that TPA can be applied as a cardiomyogenesis-promoting factor for the differentiation of mouse embryonic stem (mES) cells in vitro. The mechanism of TPA action is mediated by the induction of extracellular signal-regulated kinase (ERK) activity and the subsequent phosphorylation of GATA4 transcription factor. Interestingly, general mitogens (FGF, EGF, VEGF and serum) or canonical WNT signalling did not mimic the effect of TPA. Moreover, on the basis of our results, we postulate that a TPA-sensitive population of cardiac progenitor cells exists at a certain time point (after days 6-8 of the differentiation protocol) and that the proposed treatment can be used to increase the multiplication of ES cell-derived cardiomyocytes.

Links

GA18-18235S, research and development project

Name: Nové možnosti v kardiomyogenní diferenciaci pluripotentních kmenových buněk (Acronym: KarPKB)

Investor: Czech Science Foundation

MUNI/A/0951/2019, interní kód MU

Name: Buněčná a molekulární biologie pro Biomedicínské vědy

Investor: Masaryk University, Category A

MUNI/A/1145/2017, interní kód MU

Name: Podpora výzkumné činnosti studentů v oblasti fyziologie, vývojové biologie a imunologie živočichů v roce 2018 (Acronym: OFIŽ 2018)

Investor: Masaryk University, Category A

Citovat

RADASZKIEWICZ, Katarzyna Anna, Deborah BECKEROVÁ, Lucie WOLOSZCZUKOVÁ, Tomasz Witold RADASZKIEWICZ, Petra LESÁKOVÁ, Olga VONDÁLOVÁ BLANÁŘOVÁ, Lukáš KUBALA, Petr HUMPOLÍČEK and Jiří PACHERNÍK. 12-O-Tetradecanoylphor bol-13-acetate increases cardiomyogenesis through PKC/ERK signaling. Scientific reports. Berlin: Nature Research, 2020, vol. 10, No 1, p. 1-13. ISSN 2045-2322. Available from: https://dx.doi.org/10.1038/s41598-020-73074-4.

@article{1703696,
   author = {Radaszkiewicz, Katarzyna Anna and Beckerová, Deborah and Woloszczuková, Lucie and Radaszkiewicz, Tomasz Witold and Lesáková, Petra and Vondálová Blanářová, Olga and Kubala, Lukáš and Humpolíček, Petr and Pacherník, Jiří},
   article_location = {Berlin},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s41598-020-73074-4},
   keywords = {Cell biology; Developmental biology; Stem cells},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific reports},
   title = {12-O-Tetradecanoylphor bol-13-acetate increases cardiomyogenesis through PKC/ERK signaling},
   url = {https://doi.org/10.1038/s41598-020-73074-4},
   volume = {10},
   year = {2020}
}

TY  - JOUR
ID  - 1703696
AU  - Radaszkiewicz, Katarzyna Anna - Beckerová, Deborah - Woloszczuková, Lucie - Radaszkiewicz, Tomasz Witold - Lesáková, Petra - Vondálová Blanářová, Olga - Kubala, Lukáš - Humpolíček, Petr - Pacherník, Jiří
PY  - 2020
TI  - 12-O-Tetradecanoylphor bol-13-acetate increases cardiomyogenesis through PKC/ERK signaling
JF  - Scientific reports
VL  - 10
IS  - 1
SP  - 1-13
EP  - 1-13
PB  - Nature Research
SN  - 20452322
KW  - Cell biology
KW  - Developmental biology
KW  - Stem cells
UR  - https://doi.org/10.1038/s41598-020-73074-4
L2  - https://doi.org/10.1038/s41598-020-73074-4
N2  - 12-O-Tetradecanoylphorbol-13-acetate (TPA) is the most widely used diacylglycerol (DAG) mimetic agent and inducer of protein kinase C (PKC)-mediated cellular response in biomedical studies. TPA has been proposed as a pluripotent cell differentiation factor, but results obtained have been inconsistent. In the present study we show that TPA can be applied as a cardiomyogenesis-promoting factor for the differentiation of mouse embryonic stem (mES) cells in vitro. The mechanism of TPA action is mediated by the induction of extracellular signal-regulated kinase (ERK) activity and the subsequent phosphorylation of GATA4 transcription factor. Interestingly, general mitogens (FGF, EGF, VEGF and serum) or canonical WNT signalling did not mimic the effect of TPA. Moreover, on the basis of our results, we postulate that a TPA-sensitive population of cardiac progenitor cells exists at a certain time point (after days 6-8 of the differentiation protocol) and that the proposed treatment can be used to increase the multiplication of ES cell-derived cardiomyocytes.
ER  -

RADASZKIEWICZ, Katarzyna Anna, Deborah BECKEROVÁ, Lucie WOLOSZCZUKOVÁ, Tomasz Witold RADASZKIEWICZ, Petra LESÁKOVÁ, Olga VONDÁLOVÁ BLANÁŘOVÁ, Lukáš KUBALA, Petr HUMPOLÍČEK and Jiří PACHERNÍK. 12-O-Tetradecanoylphor bol-13-acetate increases cardiomyogenesis through PKC/ERK signaling. \textit{Scientific reports}. Berlin: Nature Research, 2020, vol.~10, No~1, p.~1-13. ISSN~2045-2322. Available from: https://dx.doi.org/10.1038/s41598-020-73074-4.

Detailed Information on Publication Record