| MANUEL IGLESIAS-PEDRAZ, Juan, Diego Matia Antonio FOSSATTI JARA, Valeria VALLE-RIESTRA-FELICE, Sergio RAFAEL CRUZ-VISALAYA, Jose Antonio Ayala FELIX and Lucio COMAI. WRN modulates translation by influencing nuclear mRNA export in HeLa cancer cells. BMC Molecular and Cell Biology. London: BMC, 2020, vol. 21, No 1, p. 1-16. ISSN 2661-8850. Available from: https://dx.doi.org/10.1186/s12860-020-00315-9. |
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@article{1704077,
author = {Manuel IglesiasandPedraz, Juan and Fossatti Jara, Diego Matia Antonio and ValleandRiestraandFelice, Valeria and Rafael CruzandVisalaya, Sergio and Felix, Jose Antonio Ayala and Comai, Lucio},
article_location = {London},
article_number = {1},
doi = {http://dx.doi.org/10.1186/s12860-020-00315-9},
keywords = {Werner syndrome protein; mRNA export; NXF1 export receptor; Translation; Cancer; Senescence},
language = {eng},
issn = {2661-8850},
journal = {BMC Molecular and Cell Biology},
title = {WRN modulates translation by influencing nuclear mRNA export in HeLa cancer cells},
url = {https://doi.org/10.1186/s12860-020-00315-9},
volume = {21},
year = {2020}
}
TY - JOUR
ID - 1704077
AU - Manuel Iglesias-Pedraz, Juan - Fossatti Jara, Diego Matia Antonio - Valle-Riestra-Felice, Valeria - Rafael Cruz-Visalaya, Sergio - Felix, Jose Antonio Ayala - Comai, Lucio
PY - 2020
TI - WRN modulates translation by influencing nuclear mRNA export in HeLa cancer cells
JF - BMC Molecular and Cell Biology
VL - 21
IS - 1
SP - 1-16
EP - 1-16
PB - BMC
SN - 26618850
KW - Werner syndrome protein
KW - mRNA export
KW - NXF1 export receptor
KW - Translation
KW - Cancer
KW - Senescence
UR - https://doi.org/10.1186/s12860-020-00315-9
L2 - https://doi.org/10.1186/s12860-020-00315-9
N2 - Background The Werner syndrome protein (WRN) belongs to the RecQ family of helicases and its loss of function results in the premature aging disease Werner syndrome (WS). We previously demonstrated that an early cellular change induced by WRN depletion is a posttranscriptional decrease in the levels of enzymes involved in metabolic pathways that control macromolecular synthesis and protect from oxidative stress. This metabolic shift is tolerated by normal cells but causes mitochondria dysfunction and acute oxidative stress in rapidly growing cancer cells, thereby suppressing their proliferation. Results To identify the mechanism underlying this metabolic shift, we examined global protein synthesis and mRNA nucleocytoplasmic distribution after WRN knockdown. We determined that WRN depletion in HeLa cells attenuates global protein synthesis without affecting the level of key components of the mRNA export machinery. We further observed that WRN depletion affects the nuclear export of mRNAs and demonstrated that WRN interacts with mRNA and the Nuclear RNA Export Factor 1 (NXF1). Conclusions Our findings suggest that WRN influences the export of mRNAs from the nucleus through its interaction with the NXF1 export receptor thereby affecting cellular proteostasis. In summary, we identified a new partner and a novel function of WRN, which is especially important for the proliferation of cancer cells.
ER -
MANUEL IGLESIAS-PEDRAZ, Juan, Diego Matia Antonio FOSSATTI JARA, Valeria VALLE-RIESTRA-FELICE, Sergio RAFAEL CRUZ-VISALAYA, Jose Antonio Ayala FELIX and Lucio COMAI. WRN modulates translation by influencing nuclear mRNA export in HeLa cancer cells. \textit{BMC Molecular and Cell Biology}. London: BMC, 2020, vol.~21, No~1, p.~1-16. ISSN~2661-8850. Available from: https://dx.doi.org/10.1186/s12860-020-00315-9.
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