Dramatic changes in the electrochemical and spectroscopic
behavior of DNA fragments induced by the loss of a single
nucleotide

J 2020

Dramatic changes in the electrochemical and spectroscopic behavior of DNA fragments induced by the loss of a single nucleotide

TŘÍSKOVÁ, Iveta, Jakub VĚŽNÍK, Karel LACINA and Libuše TRNKOVÁ

Basic information

Original name

Dramatic changes in the electrochemical and spectroscopic behavior of DNA fragments induced by the loss of a single nucleotide

Authors

TŘÍSKOVÁ, Iveta (203 Czech Republic, belonging to the institution), Jakub VĚŽNÍK (203 Czech Republic, belonging to the institution), Karel LACINA (203 Czech Republic, belonging to the institution) and Libuše TRNKOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Bioelectrochemistry, Lausanne, Elsevier, 2020, 1567-5394

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.373

RIV identification code

RIV/00216224:14310/20:00117236

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.bioelechem.2020.107515

UT WoS

000579727300013

Keywords in English

Oligodeoxynucleotide structure; Hairpin; Duplex; GA mismatches; Voltammetry; CD spectra

Abstract

V originále

In this paper, the d(GCGAAGC) heptamer and the closely related d(GCGAGC) hexamer are examined via electrochemical (cyclic voltammetry) and spectroscopic (circular dichroism) methods. Dramatic changes in the CD spectroscopic and CV electrochemical properties, induced by the loss of only one single nucleotide (A), are detected. The CD spectra and native polyacrylamide gel electrophoresis (PAGE) confirmed structural changes taking place in the relevant chain-like oligodeoxynucleotide assemblies. Dedicated studies suggest that the heptamer (Hp) possesses a hairpin structure, whereas the hexamer (Hx) appears to be rather a duplex. Both of the structures exhibited completely different adsorption behavior at the hanging mercury drop electrode, and this factor was readily confirmed by means of elimination voltammetry with linear scan (EVLS). We established that the Hp hairpin (similar to-1300 mV), compared to the Hx duplex (similar to-1360 mV), is the thermodynamically favored electron acceptor. The adsorption isotherms were constructed based on the voltammetric peak height values, reflecting the reduction of the adenine (A) and cytosine (C) moieties as well as the oxidation of the 7,8-dihydroguanine (7,8-DHG) moieties. Finally, as revealed by the spectroscopic and electrochemical results, Hx forms a bimolecular antiparallel homo-duplex carrying both Watson-Crick base pairs (CG or GC) and mismatched edge-to-edge base pairs (GA or AG). (C) 2020 Published by Elsevier B.V.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/1359/2018, interní kód MU

Name: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2019

Investor: Masaryk University, Category A

Citovat

TŘÍSKOVÁ, Iveta, Jakub VĚŽNÍK, Karel LACINA and Libuše TRNKOVÁ. Dramatic changes in the electrochemical and spectroscopic behavior of DNA fragments induced by the loss of a single nucleotide. Bioelectrochemistry. Lausanne: Elsevier, 2020, vol. 134, August 2020, p. 1-8. ISSN 1567-5394. Available from: https://dx.doi.org/10.1016/j.bioelechem.2020.107515.

@article{1704220,
   author = {Třísková, Iveta and Věžník, Jakub and Lacina, Karel and Trnková, Libuše},
   article_location = {Lausanne},
   article_number = {August 2020},
   doi = {http://dx.doi.org/10.1016/j.bioelechem.2020.107515},
   keywords = {Oligodeoxynucleotide structure; Hairpin; Duplex; GA mismatches; Voltammetry; CD spectra},
   language = {eng},
   issn = {1567-5394},
   journal = {Bioelectrochemistry},
   note = {Vykazovat pod PřF},
   title = {Dramatic changes in the electrochemical and spectroscopic behavior of DNA fragments induced by the loss of a single nucleotide},
   url = {https://doi.org/10.1016/j.bioelechem.2020.107515},
   volume = {134},
   year = {2020}
}

TY  - JOUR
ID  - 1704220
AU  - Třísková, Iveta - Věžník, Jakub - Lacina, Karel - Trnková, Libuše
PY  - 2020
TI  - Dramatic changes in the electrochemical and spectroscopic behavior of DNA fragments induced by the loss of a single nucleotide
JF  - Bioelectrochemistry
VL  - 134
IS  - August 2020
SP  - 1-8
EP  - 1-8
PB  - Elsevier
SN  - 15675394
N1  - Vykazovat pod PřF
KW  - Oligodeoxynucleotide structure
KW  - Hairpin
KW  - Duplex
KW  - GA mismatches
KW  - Voltammetry
KW  - CD spectra
UR  - https://doi.org/10.1016/j.bioelechem.2020.107515
L2  - https://doi.org/10.1016/j.bioelechem.2020.107515
N2  - In this paper, the d(GCGAAGC) heptamer and the closely related d(GCGAGC) hexamer are examined via electrochemical (cyclic voltammetry) and spectroscopic (circular dichroism) methods. Dramatic changes in the CD spectroscopic and CV electrochemical properties, induced by the loss of only one single nucleotide (A), are detected. The CD spectra and native polyacrylamide gel electrophoresis (PAGE) confirmed structural changes taking place in the relevant chain-like oligodeoxynucleotide assemblies. Dedicated studies suggest that the heptamer (Hp) possesses a hairpin structure, whereas the hexamer (Hx) appears to be rather a duplex. Both of the structures exhibited completely different adsorption behavior at the hanging mercury drop electrode, and this factor was readily confirmed by means of elimination voltammetry with linear scan (EVLS). We established that the Hp hairpin (similar to-1300 mV), compared to the Hx duplex (similar to-1360 mV), is the thermodynamically favored electron acceptor. The adsorption isotherms were constructed based on the voltammetric peak height values, reflecting the reduction of the adenine (A) and cytosine (C) moieties as well as the oxidation of the 7,8-dihydroguanine (7,8-DHG) moieties. Finally, as revealed by the spectroscopic and electrochemical results, Hx forms a bimolecular antiparallel homo-duplex carrying both Watson-Crick base pairs (CG or GC) and mismatched edge-to-edge base pairs (GA or AG). (C) 2020 Published by Elsevier B.V.
ER  -

TŘÍSKOVÁ, Iveta, Jakub VĚŽNÍK, Karel LACINA and Libuše TRNKOVÁ. Dramatic changes in the electrochemical and spectroscopic behavior of DNA fragments induced by the loss of a single nucleotide. \textit{Bioelectrochemistry}. Lausanne: Elsevier, 2020, vol.~134, August 2020, p.~1-8. ISSN~1567-5394. Available from: https://dx.doi.org/10.1016/j.bioelechem.2020.107515.

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