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@article{1704220, author = {Třísková, Iveta and Věžník, Jakub and Lacina, Karel and Trnková, Libuše}, article_location = {Lausanne}, article_number = {August 2020}, doi = {http://dx.doi.org/10.1016/j.bioelechem.2020.107515}, keywords = {Oligodeoxynucleotide structure; Hairpin; Duplex; GA mismatches; Voltammetry; CD spectra}, language = {eng}, issn = {1567-5394}, journal = {Bioelectrochemistry}, note = {Vykazovat pod PřF}, title = {Dramatic changes in the electrochemical and spectroscopic behavior of DNA fragments induced by the loss of a single nucleotide}, url = {https://doi.org/10.1016/j.bioelechem.2020.107515}, volume = {134}, year = {2020} }
TY - JOUR ID - 1704220 AU - Třísková, Iveta - Věžník, Jakub - Lacina, Karel - Trnková, Libuše PY - 2020 TI - Dramatic changes in the electrochemical and spectroscopic behavior of DNA fragments induced by the loss of a single nucleotide JF - Bioelectrochemistry VL - 134 IS - August 2020 SP - 1-8 EP - 1-8 PB - Elsevier SN - 15675394 N1 - Vykazovat pod PřF KW - Oligodeoxynucleotide structure KW - Hairpin KW - Duplex KW - GA mismatches KW - Voltammetry KW - CD spectra UR - https://doi.org/10.1016/j.bioelechem.2020.107515 L2 - https://doi.org/10.1016/j.bioelechem.2020.107515 N2 - In this paper, the d(GCGAAGC) heptamer and the closely related d(GCGAGC) hexamer are examined via electrochemical (cyclic voltammetry) and spectroscopic (circular dichroism) methods. Dramatic changes in the CD spectroscopic and CV electrochemical properties, induced by the loss of only one single nucleotide (A), are detected. The CD spectra and native polyacrylamide gel electrophoresis (PAGE) confirmed structural changes taking place in the relevant chain-like oligodeoxynucleotide assemblies. Dedicated studies suggest that the heptamer (Hp) possesses a hairpin structure, whereas the hexamer (Hx) appears to be rather a duplex. Both of the structures exhibited completely different adsorption behavior at the hanging mercury drop electrode, and this factor was readily confirmed by means of elimination voltammetry with linear scan (EVLS). We established that the Hp hairpin (similar to-1300 mV), compared to the Hx duplex (similar to-1360 mV), is the thermodynamically favored electron acceptor. The adsorption isotherms were constructed based on the voltammetric peak height values, reflecting the reduction of the adenine (A) and cytosine (C) moieties as well as the oxidation of the 7,8-dihydroguanine (7,8-DHG) moieties. Finally, as revealed by the spectroscopic and electrochemical results, Hx forms a bimolecular antiparallel homo-duplex carrying both Watson-Crick base pairs (CG or GC) and mismatched edge-to-edge base pairs (GA or AG). (C) 2020 Published by Elsevier B.V. ER -
TŘÍSKOVÁ, Iveta, Jakub VĚŽNÍK, Karel LACINA and Libuše TRNKOVÁ. Dramatic changes in the electrochemical and spectroscopic behavior of DNA fragments induced by the loss of a single nucleotide. \textit{Bioelectrochemistry}. Lausanne: Elsevier, 2020, vol.~134, August 2020, p.~1-8. ISSN~1567-5394. Available from: https://dx.doi.org/10.1016/j.bioelechem.2020.107515.
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