Analysis of bile acids in human biological samples by
microcolumn separation techniques: A review

J 2021

Analysis of bile acids in human biological samples by microcolumn separation techniques: A review

DOSEDĚLOVÁ, Věra, Petra ITTERHEIMOVÁ and Petr KUBÁŇ

Basic information

Original name

Analysis of bile acids in human biological samples by microcolumn separation techniques: A review

Authors

DOSEDĚLOVÁ, Věra (203 Czech Republic, guarantor, belonging to the institution), Petra ITTERHEIMOVÁ (203 Czech Republic, belonging to the institution) and Petr KUBÁŇ (203 Czech Republic)

Edition

Electrophoresis, Hoboken, Wiley, 2021, 0173-0835

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.595

RIV identification code

RIV/00216224:14740/21:00120079

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1002/elps.202000139

UT WoS

000552288300001

Keywords in English

Bile acids; Clinical analysis; GC-MS; HPLC-MS; Microcolumn separation

Abstract

V originále

Bile acids are a group of compounds essential for lipid digestion and absorption with a steroid skeleton and a carboxylate side chain usually conjugated to glycine or taurine. Bile acids are regulatory molecules for a number of metabolic processes and can be used as biomarkers of various disorders. Since the middle of the twentieth century, the detection of bile acids has evolved from simple qualitative analysis to accurate quantification in complicated mixtures. Advanced methods are required to characterize and quantify individual bile acids in these mixtures. This article overviews the literature from the last two decades (2000-2020) and focuses on bile acid analysis in various human biological samples. The methods for sample preparation, including the sample treatment of conventional (blood plasma, blood serum, and urine) and unconventional samples (bile, saliva, duodenal/gastric juice, feces, etc.) are shortly discussed. Eventually, the focus is on novel analytical approaches and methods for each particular biological sample, providing an overview of the microcolumn separation techniques, such as high-performance liquid chromatography, gas chromatography, and capillary electrophoresis, used in their analysis. This is followed by a discussion on selected clinical applications.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

NV17-31945A, research and development project

Name: Vývoj neinvazivní diagnostiky extraezofageálních projevů refluxní choroby jícnu

Citovat

DOSEDĚLOVÁ, Věra, Petra ITTERHEIMOVÁ and Petr KUBÁŇ. Analysis of bile acids in human biological samples by microcolumn separation techniques: A review. Electrophoresis. Hoboken: Wiley, 2021, vol. 42, 1-2, p. 68-85. ISSN 0173-0835. Available from: https://dx.doi.org/10.1002/elps.202000139.

@article{1707540,
   author = {Dosedělová, Věra and Itterheimová, Petra and Kubáň, Petr},
   article_location = {Hoboken},
   article_number = {1-2},
   doi = {http://dx.doi.org/10.1002/elps.202000139},
   keywords = {Bile acids; Clinical analysis; GC-MS; HPLC-MS; Microcolumn separation},
   language = {eng},
   issn = {0173-0835},
   journal = {Electrophoresis},
   title = {Analysis of bile acids in human biological samples by microcolumn separation techniques: A review},
   url = {https://doi.org/10.1002/elps.202000139},
   volume = {42},
   year = {2021}
}

TY  - JOUR
ID  - 1707540
AU  - Dosedělová, Věra - Itterheimová, Petra - Kubáň, Petr
PY  - 2021
TI  - Analysis of bile acids in human biological samples by microcolumn separation techniques: A review
JF  - Electrophoresis
VL  - 42
IS  - 1-2
SP  - 68-85
EP  - 68-85
PB  - Wiley
SN  - 01730835
KW  - Bile acids
KW  - Clinical analysis
KW  - GC-MS
KW  - HPLC-MS
KW  - Microcolumn separation
UR  - https://doi.org/10.1002/elps.202000139
L2  - https://doi.org/10.1002/elps.202000139
N2  - Bile acids are a group of compounds essential for lipid digestion and absorption with a steroid skeleton and a carboxylate side chain usually conjugated to glycine or taurine. Bile acids are regulatory molecules for a number of metabolic processes and can be used as biomarkers of various disorders. Since the middle of the twentieth century, the detection of bile acids has evolved from simple qualitative analysis to accurate quantification in complicated mixtures. Advanced methods are required to characterize and quantify individual bile acids in these mixtures. This article overviews the literature from the last two decades (2000-2020) and focuses on bile acid analysis in various human biological samples. The methods for sample preparation, including the sample treatment of conventional (blood plasma, blood serum, and urine) and unconventional samples (bile, saliva, duodenal/gastric juice, feces, etc.) are shortly discussed. Eventually, the focus is on novel analytical approaches and methods for each particular biological sample, providing an overview of the microcolumn separation techniques, such as high-performance liquid chromatography, gas chromatography, and capillary electrophoresis, used in their analysis. This is followed by a discussion on selected clinical applications.
ER  -

DOSEDĚLOVÁ, Věra, Petra ITTERHEIMOVÁ and Petr KUBÁŇ. Analysis of bile acids in human biological samples by microcolumn separation techniques: A review. \textit{Electrophoresis}. Hoboken: Wiley, 2021, vol.~42, 1-2, p.~68-85. ISSN~0173-0835. Available from: https://dx.doi.org/10.1002/elps.202000139.

Detailed Information on Publication Record