Analysis of major bile acids in saliva samples of patients with
Barrett's esophagus using high-performance liquid
chromatography-electrospray ionization-mass spectrometry

J 2020

Analysis of major bile acids in saliva samples of patients with Barrett's esophagus using high-performance liquid chromatography-electrospray ionization-mass spectrometry

ĎURČ, Pavol, Věra DOSEDĚLOVÁ, František FORET, Jiří DOLINA, Štefan KONEČNÝ et. al.

Basic information

Original name

Analysis of major bile acids in saliva samples of patients with Barrett's esophagus using high-performance liquid chromatography-electrospray ionization-mass spectrometry

Authors

ĎURČ, Pavol (703 Slovakia, belonging to the institution), Věra DOSEDĚLOVÁ (203 Czech Republic, belonging to the institution), František FORET (203 Czech Republic, belonging to the institution), Jiří DOLINA (203 Czech Republic, belonging to the institution), Štefan KONEČNÝ (703 Slovakia, belonging to the institution), Markus HIMMELSBACH, Wolfgang BUCHBERGER and Petr KUBÁŇ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Chromatography A, Amsterdam, Elsevier, 2020, 0021-9673

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.759

RIV identification code

RIV/00216224:14740/20:00117316

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.chroma.2020.461278

UT WoS

000552017600024

Keywords in English

Bile acids; HPLC-MS; Saliva; Barrett's esophagus

Abstract

V originále

A fast, non-invasive, high-performance liquid chromatographic screening method with electrospray ionization mass spectrometric detection was developed for the analysis of three major glycine-conjugated bile acids in human saliva. Using a mobile phase composed of 80% methanol and 0.1% formic acid, glycocholic, glycodeoxycholic, and glycochenodeoxycholic acids were separated in less than 4 minutes with sensitivity in the low nM range. Bile acids are thought to contribute to the pathology of various complications in gastroesophageal reflux disease, for instance, Barrett's esophagus, which may eventually lead to esophageal carcinoma. In this pilot study, samples of saliva obtained from 15 patients with Barrett's esophagus of various severities were compared to saliva samples from 10 healthy volunteers. Glycochenodeoxycholic acid was significantly elevated in the patients and principal component analysis of all bile acids could distinguish the most severe Barrett's esophagus patients. We also reported on the detection of glycochenodeoxycholic acid in exhaled breath condensate for the first time. The promising results of this pilot study warrant future investigation, aiming at non-invasive diagnostics of Barrett's esophagus susceptibility in patients with gastroesophageal reflux disease.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

NV17-31945A, research and development project

Name: Vývoj neinvazivní diagnostiky extraezofageálních projevů refluxní choroby jícnu

Citovat

ĎURČ, Pavol, Věra DOSEDĚLOVÁ, František FORET, Jiří DOLINA, Štefan KONEČNÝ, Markus HIMMELSBACH, Wolfgang BUCHBERGER and Petr KUBÁŇ. Analysis of major bile acids in saliva samples of patients with Barrett's esophagus using high-performance liquid chromatography-electrospray ionization-mass spectrometry. Journal of Chromatography A. Amsterdam: Elsevier, 2020, vol. 1625, August 2020, p. 1-9. ISSN 0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2020.461278.

@article{1707542,
   author = {Ďurč, Pavol and Dosedělová, Věra and Foret, František and Dolina, Jiří and Konečný, Štefan and Himmelsbach, Markus and Buchberger, Wolfgang and Kubáň, Petr},
   article_location = {Amsterdam},
   article_number = {August 2020},
   doi = {http://dx.doi.org/10.1016/j.chroma.2020.461278},
   keywords = {Bile acids; HPLC-MS; Saliva; Barrett's esophagus},
   language = {eng},
   issn = {0021-9673},
   journal = {Journal of Chromatography A},
   title = {Analysis of major bile acids in saliva samples of patients with Barrett's esophagus using high-performance liquid chromatography-electrospray ionization-mass spectrometry},
   url = {https://doi.org/10.1016/j.chroma.2020.461278},
   volume = {1625},
   year = {2020}
}

TY  - JOUR
ID  - 1707542
AU  - Ďurč, Pavol - Dosedělová, Věra - Foret, František - Dolina, Jiří - Konečný, Štefan - Himmelsbach, Markus - Buchberger, Wolfgang - Kubáň, Petr
PY  - 2020
TI  - Analysis of major bile acids in saliva samples of patients with Barrett's esophagus using high-performance liquid chromatography-electrospray ionization-mass spectrometry
JF  - Journal of Chromatography A
VL  - 1625
IS  - August 2020
SP  - 1-9
EP  - 1-9
PB  - Elsevier
SN  - 00219673
KW  - Bile acids
KW  - HPLC-MS
KW  - Saliva
KW  - Barrett's esophagus
UR  - https://doi.org/10.1016/j.chroma.2020.461278
L2  - https://doi.org/10.1016/j.chroma.2020.461278
N2  - A fast, non-invasive, high-performance liquid chromatographic screening method with electrospray ionization mass spectrometric detection was developed for the analysis of three major glycine-conjugated bile acids in human saliva. Using a mobile phase composed of 80% methanol and 0.1% formic acid, glycocholic, glycodeoxycholic, and glycochenodeoxycholic acids were separated in less than 4 minutes with sensitivity in the low nM range. Bile acids are thought to contribute to the pathology of various complications in gastroesophageal reflux disease, for instance, Barrett's esophagus, which may eventually lead to esophageal carcinoma. In this pilot study, samples of saliva obtained from 15 patients with Barrett's esophagus of various severities were compared to saliva samples from 10 healthy volunteers. Glycochenodeoxycholic acid was significantly elevated in the patients and principal component analysis of all bile acids could distinguish the most severe Barrett's esophagus patients. We also reported on the detection of glycochenodeoxycholic acid in exhaled breath condensate for the first time. The promising results of this pilot study warrant future investigation, aiming at non-invasive diagnostics of Barrett's esophagus susceptibility in patients with gastroesophageal reflux disease.
ER  -

ĎURČ, Pavol, Věra DOSEDĚLOVÁ, František FORET, Jiří DOLINA, Štefan KONEČNÝ, Markus HIMMELSBACH, Wolfgang BUCHBERGER and Petr KUBÁŇ. Analysis of major bile acids in saliva samples of patients with Barrett's esophagus using high-performance liquid chromatography-electrospray ionization-mass spectrometry. \textit{Journal of Chromatography A}. Amsterdam: Elsevier, 2020, vol.~1625, August 2020, p.~1-9. ISSN~0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2020.461278.

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