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@article{1707542,
author = {Ďurč, Pavol and Dosedělová, Věra and Foret, František and Dolina, Jiří and Konečný, Štefan and Himmelsbach, Markus and Buchberger, Wolfgang and Kubáň, Petr},
article_location = {Amsterdam},
article_number = {August 2020},
doi = {http://dx.doi.org/10.1016/j.chroma.2020.461278},
keywords = {Bile acids; HPLC-MS; Saliva; Barrett's esophagus},
language = {eng},
issn = {0021-9673},
journal = {Journal of Chromatography A},
title = {Analysis of major bile acids in saliva samples of patients with Barrett's esophagus using high-performance liquid chromatography-electrospray ionization-mass spectrometry},
url = {https://doi.org/10.1016/j.chroma.2020.461278},
volume = {1625},
year = {2020}
}
TY - JOUR
ID - 1707542
AU - Ďurč, Pavol - Dosedělová, Věra - Foret, František - Dolina, Jiří - Konečný, Štefan - Himmelsbach, Markus - Buchberger, Wolfgang - Kubáň, Petr
PY - 2020
TI - Analysis of major bile acids in saliva samples of patients with Barrett's esophagus using high-performance liquid chromatography-electrospray ionization-mass spectrometry
JF - Journal of Chromatography A
VL - 1625
IS - August 2020
SP - 1-9
EP - 1-9
PB - Elsevier
SN - 00219673
KW - Bile acids
KW - HPLC-MS
KW - Saliva
KW - Barrett's esophagus
UR - https://doi.org/10.1016/j.chroma.2020.461278
L2 - https://doi.org/10.1016/j.chroma.2020.461278
N2 - A fast, non-invasive, high-performance liquid chromatographic screening method with electrospray ionization mass spectrometric detection was developed for the analysis of three major glycine-conjugated bile acids in human saliva. Using a mobile phase composed of 80% methanol and 0.1% formic acid, glycocholic, glycodeoxycholic, and glycochenodeoxycholic acids were separated in less than 4 minutes with sensitivity in the low nM range. Bile acids are thought to contribute to the pathology of various complications in gastroesophageal reflux disease, for instance, Barrett's esophagus, which may eventually lead to esophageal carcinoma. In this pilot study, samples of saliva obtained from 15 patients with Barrett's esophagus of various severities were compared to saliva samples from 10 healthy volunteers. Glycochenodeoxycholic acid was significantly elevated in the patients and principal component analysis of all bile acids could distinguish the most severe Barrett's esophagus patients. We also reported on the detection of glycochenodeoxycholic acid in exhaled breath condensate for the first time. The promising results of this pilot study warrant future investigation, aiming at non-invasive diagnostics of Barrett's esophagus susceptibility in patients with gastroesophageal reflux disease.
ER -
ĎURČ, Pavol, Věra DOSEDĚLOVÁ, František FORET, Jiří DOLINA, Štefan KONEČNÝ, Markus HIMMELSBACH, Wolfgang BUCHBERGER and Petr KUBÁŇ. Analysis of major bile acids in saliva samples of patients with Barrett's esophagus using high-performance liquid chromatography-electrospray ionization-mass spectrometry. \textit{Journal of Chromatography A}. Amsterdam: Elsevier, 2020, vol.~1625, August 2020, p.~1-9. ISSN~0021-9673. Available from: https://dx.doi.org/10.1016/j.chroma.2020.461278.
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