Arabidopsis Flippases Cooperate with ARF GTPase Exchange
Factors to Regulate the Trafficking and Polarity of PIN Auxin
Transporters

J 2020

Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters

ZHANG, Xixi, Maciek ADAMOWSKI, Petra MARHAVA, Shutang TAN, Yuzhou ZHANG et. al.

Basic information

Original name

Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters

Authors

ZHANG, Xixi, Maciek ADAMOWSKI, Petra MARHAVA, Shutang TAN, Yuzhou ZHANG, Lesia RODRIGUEZ, Marta ZWIEWKA (616 Poland, belonging to the institution), Vendula PUKYŠOVÁ (203 Czech Republic, belonging to the institution), Asier SANCHEZ CERDAN (724 Spain, belonging to the institution), Vivek Kumar RAXWAL (356 India, belonging to the institution), Christian S. HARDTKE, Tomasz NODZYNSKI (616 Poland, guarantor, belonging to the institution) and Jiří FRIML

Edition

Plant Cell, Rockville, American Society of Plant Biologists, 2020, 1040-4651

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 11.277

RIV identification code

RIV/00216224:14740/20:00114574

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1105/tpc.19.00869

UT WoS

000545741500030

Keywords in English

P-TYPE ATPASE; TRANS-GOLGI NETWORK; APICAL-BASAL AXIS; GENE-EXPRESSION; PHOSPHOLIPID FLIPPASES; MEDIATES ENDOCYTOSIS; MEMBRANE TRAFFICKING; VACUOLAR TRAFFICKING; PROTEIN TRAFFICKING; BETA-SUBUNIT

Abstract

V originále

Cell polarity is a fundamental feature of all multicellular organisms. PIN auxin transporters are important cell polarity markers that play crucial roles in a plethora of developmental processes in plants. Here, to identify components involved in cell polarity establishment and maintenance in plants, we performed a forward genetic screening of PIN2:PIN1-HA;pin2 Arabidopsis (Arabidopsis thaliana) plants, which ectopically express predominantly basally localized PIN1 in root epidermal cells, leading to agravitropic root growth. We identified the regulator of PIN polarity 12 (repp12) mutation, which restored gravitropic root growth and caused a switch in PIN1-HA polarity from the basal to apical side of root epidermal cells. Next Generation Sequencing and complementation experiments established the causative mutation of repp12 as a single amino acid exchange in Aminophospholipid ATPase3 (ALA3), a phospholipid flippase predicted to function in vesicle formation. repp12 and ala3 T-DNA mutants show defects in many auxin-regulated processes, asymmetric auxin distribution, and PIN trafficking. Analysis of quintuple and sextuple mutants confirmed the crucial roles of ALA proteins in regulating plant development as well as PIN trafficking and polarity. Genetic and physical interaction studies revealed that ALA3 functions together with the ADP ribosylation factor GTPase exchange factors GNOM and BIG3 in regulating PIN polarity, trafficking, and auxin-mediated development.

Links

EF15_003/0000479, research and development project

Name: Regulace rostlinné meiózy

GA18-26981S, research and development project

Name: Genetické studie k objasnění molekulárního mechanizmu účinku strigolaktonů v kořeni

Investor: Czech Science Foundation

GJ17-17966Y, research and development project

Name: Charakteristika nových molekulárních komponentů podílejících se na distribuci proteinů v rostlinách.

90129, large research infrastructures

Name: Czech-BioImaging II

Citovat

ZHANG, Xixi, Maciek ADAMOWSKI, Petra MARHAVA, Shutang TAN, Yuzhou ZHANG, Lesia RODRIGUEZ, Marta ZWIEWKA, Vendula PUKYŠOVÁ, Asier SANCHEZ CERDAN, Vivek Kumar RAXWAL, Christian S. HARDTKE, Tomasz NODZYNSKI and Jiří FRIML. Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters. Plant Cell. Rockville: American Society of Plant Biologists, 2020, vol. 32, No 5, p. 1644-1664. ISSN 1040-4651. Available from: https://dx.doi.org/10.1105/tpc.19.00869.

@article{1710076,
   author = {Zhang, Xixi and Adamowski, Maciek and Marhava, Petra and Tan, Shutang and Zhang, Yuzhou and Rodriguez, Lesia and Zwiewka, Marta and Pukyšová, Vendula and Sanchez Cerdan, Asier and Raxwal, Vivek Kumar and Hardtke, Christian S. and Nodzynski, Tomasz and Friml, Jiří},
   article_location = {Rockville},
   article_number = {5},
   doi = {http://dx.doi.org/10.1105/tpc.19.00869},
   keywords = {P-TYPE ATPASE; TRANS-GOLGI NETWORK; APICAL-BASAL AXIS; GENE-EXPRESSION; PHOSPHOLIPID FLIPPASES; MEDIATES ENDOCYTOSIS; MEMBRANE TRAFFICKING; VACUOLAR TRAFFICKING; PROTEIN TRAFFICKING; BETA-SUBUNIT},
   language = {eng},
   issn = {1040-4651},
   journal = {Plant Cell},
   title = {Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters},
   url = {https://doi.org/10.1105/tpc.19.00869},
   volume = {32},
   year = {2020}
}

TY  - JOUR
ID  - 1710076
AU  - Zhang, Xixi - Adamowski, Maciek - Marhava, Petra - Tan, Shutang - Zhang, Yuzhou - Rodriguez, Lesia - Zwiewka, Marta - Pukyšová, Vendula - Sanchez Cerdan, Asier - Raxwal, Vivek Kumar - Hardtke, Christian S. - Nodzynski, Tomasz - Friml, Jiří
PY  - 2020
TI  - Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters
JF  - Plant Cell
VL  - 32
IS  - 5
SP  - 1644-1664
EP  - 1644-1664
PB  - American Society of Plant Biologists
SN  - 10404651
KW  - P-TYPE ATPASE
KW  - TRANS-GOLGI NETWORK
KW  - APICAL-BASAL AXIS
KW  - GENE-EXPRESSION
KW  - PHOSPHOLIPID FLIPPASES
KW  - MEDIATES ENDOCYTOSIS
KW  - MEMBRANE TRAFFICKING
KW  - VACUOLAR TRAFFICKING
KW  - PROTEIN TRAFFICKING
KW  - BETA-SUBUNIT
UR  - https://doi.org/10.1105/tpc.19.00869
L2  - https://doi.org/10.1105/tpc.19.00869
N2  - Cell polarity is a fundamental feature of all multicellular organisms. PIN auxin transporters are important cell polarity markers that play crucial roles in a plethora of developmental processes in plants. Here, to identify components involved in cell polarity establishment and maintenance in plants, we performed a forward genetic screening of PIN2:PIN1-HA;pin2 Arabidopsis (Arabidopsis thaliana) plants, which ectopically express predominantly basally localized PIN1 in root epidermal cells, leading to agravitropic root growth. We identified the regulator of PIN polarity 12 (repp12) mutation, which restored gravitropic root growth and caused a switch in PIN1-HA polarity from the basal to apical side of root epidermal cells. Next Generation Sequencing and complementation experiments established the causative mutation of repp12 as a single amino acid exchange in Aminophospholipid ATPase3 (ALA3), a phospholipid flippase predicted to function in vesicle formation. repp12 and ala3 T-DNA mutants show defects in many auxin-regulated processes, asymmetric auxin distribution, and PIN trafficking. Analysis of quintuple and sextuple mutants confirmed the crucial roles of ALA proteins in regulating plant development as well as PIN trafficking and polarity. Genetic and physical interaction studies revealed that ALA3 functions together with the ADP ribosylation factor GTPase exchange factors GNOM and BIG3 in regulating PIN polarity, trafficking, and auxin-mediated development.
ER  -

ZHANG, Xixi, Maciek ADAMOWSKI, Petra MARHAVA, Shutang TAN, Yuzhou ZHANG, Lesia RODRIGUEZ, Marta ZWIEWKA, Vendula PUKYŠOVÁ, Asier SANCHEZ CERDAN, Vivek Kumar RAXWAL, Christian S. HARDTKE, Tomasz NODZYNSKI and Jiří FRIML. Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters. \textit{Plant Cell}. Rockville: American Society of Plant Biologists, 2020, vol.~32, No~5, p.~1644-1664. ISSN~1040-4651. Available from: https://dx.doi.org/10.1105/tpc.19.00869.

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