Quadruplex Priming Amplification at a Range of Human Body
Temperature

J 2021

Quadruplex Priming Amplification at a Range of Human Body Temperature

GVARJALADZE, David; Tamari GULUA; Shota GOGICHAISHVILI; Jozef HRITZ; Nunu METREVEL et. al.

Základní údaje

Originální název

Quadruplex Priming Amplification at a Range of Human Body Temperature

Autoři

GVARJALADZE, David; Tamari GULUA; Shota GOGICHAISHVILI; Jozef HRITZ a Nunu METREVEL

Vydání

Biointerface Research in Applied Chemistry, Bucuresti, AMG Transcend Association, 2021, 2069-5837

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10403 Physical chemistry

Stát vydavatele

Rumunsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14740/21:00120870

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.33263/BRIAC111.79327942

UT WoS

000570974900052

EID Scopus

2-s2.0-85090253841

Klíčová slova anglicky

quadruplex; DNA amplification; the point of care; lead cations; fluorescence

Štítky

14313010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 30. 1. 2024 09:46, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Quadruplex priming amplification (QPA) is a very simple amplification assay in which the isothermal amplification is performed using an only DNA polymerase, and detection is conducted by the intrinsic fluorescence of the primers. QPA employs specific G-rich sequences as primers that, upon polymerase elongation at specific temperatures, spontaneously dissociate from the primer binding sites (PBS) and fold into a monomolecular quadruplex. Fluorescent nucleotide analogs, such as 3-methyl isoxanthopterin (3MI), when incorporated into these primers, emit light upon a quadruplex formation and permit simple, specific, and sensitive quantification without the attachment of probe molecules. Previously has developed QPA assays with truncated targets and potassium cations that demonstrate an optimal amplification around 55 degrees C. Here, we designed QPA assays with truncated target and led cations at a range of human body temperature. Lead cations reveal the most rapid amplification than potassium and strontium cations. QPA can be applied as a method for the implementation of simple and cheap diagnostics (point of care (POC)), as well as development at a range of 35-45 degrees C temperature, which will make this method more convenient for using it in molecular diagnostics.

Citovat

GVARJALADZE, David; Tamari GULUA; Shota GOGICHAISHVILI; Jozef HRITZ a Nunu METREVEL. Quadruplex Priming Amplification at a Range of Human Body Temperature. Biointerface Research in Applied Chemistry. Bucuresti: AMG Transcend Association, 2021, roč. 11, č. 1, s. 7932-7942. ISSN 2069-5837. Dostupné z: https://doi.org/10.33263/BRIAC111.79327942.

@article{1710336,
   author = {Gvarjaladze, David and Gulua, Tamari and Gogichaishvili, Shota and Hritz, Jozef and Metrevel, Nunu},
   article_location = {Bucuresti},
   article_number = {1},
   doi = {https://doi.org/10.33263/BRIAC111.79327942},
   keywords = {quadruplex; DNA amplification; the point of care; lead cations; fluorescence},
   language = {eng},
   issn = {2069-5837},
   journal = {Biointerface Research in Applied Chemistry},
   title = {Quadruplex Priming Amplification at a Range of Human Body Temperature},
   url = {https://doi.org/10.33263/BRIAC111.79327942},
   volume = {11},
   year = {2021}
}

TY  - JOUR
ID  - 1710336
AU  - Gvarjaladze, David - Gulua, Tamari - Gogichaishvili, Shota - Hritz, Jozef - Metrevel, Nunu
PY  - 2021
TI  - Quadruplex Priming Amplification at a Range of Human Body Temperature
JF  - Biointerface Research in Applied Chemistry
VL  - 11
IS  - 1
SP  - 7932-7942
EP  - 7932-7942
PB  - AMG Transcend Association
SN  - 20695837
KW  - quadruplex
KW  - DNA amplification
KW  - the point of care
KW  - lead cations
KW  - fluorescence
UR  - https://doi.org/10.33263/BRIAC111.79327942
L2  - https://doi.org/10.33263/BRIAC111.79327942
N2  - Quadruplex priming amplification (QPA) is a very simple amplification assay in which the isothermal amplification is performed using an only DNA polymerase, and detection is conducted by the intrinsic fluorescence of the primers. QPA employs specific G-rich sequences as primers that, upon polymerase elongation at specific temperatures, spontaneously dissociate from the primer binding sites (PBS) and fold into a monomolecular quadruplex. Fluorescent nucleotide analogs, such as 3-methyl isoxanthopterin (3MI), when incorporated into these primers, emit light upon a quadruplex formation and permit simple, specific, and sensitive quantification without the attachment of probe molecules. Previously has developed QPA assays with truncated targets and potassium cations that demonstrate an optimal amplification around 55 degrees C. Here, we designed QPA assays with truncated target and led cations at a range of human body temperature. Lead cations reveal the most rapid amplification than potassium and strontium cations. QPA can be applied as a method for the implementation of simple and cheap diagnostics (point of care (POC)), as well as development at a range of 35-45 degrees C temperature, which will make this method more convenient for using it in molecular diagnostics.
ER  -

GVARJALADZE, David; Tamari GULUA; Shota GOGICHAISHVILI; Jozef HRITZ a Nunu METREVEL. Quadruplex Priming Amplification at a Range of Human Body Temperature. \textit{Biointerface Research in Applied Chemistry}. Bucuresti: AMG Transcend Association, 2021, roč.~11, č.~1, s.~7932-7942. ISSN~2069-5837. Dostupné z: https://doi.org/10.33263/BRIAC111.79327942.

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