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@article{1710336, author = {Gvarjaladze, David and Gulua, Tamari and Gogichaishvili, Shota and Hritz, Jozef and Metrevel, Nunu}, article_location = {Bucuresti}, article_number = {1}, doi = {http://dx.doi.org/10.33263/BRIAC111.79327942}, keywords = {quadruplex; DNA amplification; the point of care; lead cations; fluorescence}, language = {eng}, issn = {2069-5837}, journal = {Biointerface Research in Applied Chemistry}, title = {Quadruplex Priming Amplification at a Range of Human Body Temperature}, url = {https://doi.org/10.33263/BRIAC111.79327942}, volume = {11}, year = {2021} }
TY - JOUR ID - 1710336 AU - Gvarjaladze, David - Gulua, Tamari - Gogichaishvili, Shota - Hritz, Jozef - Metrevel, Nunu PY - 2021 TI - Quadruplex Priming Amplification at a Range of Human Body Temperature JF - Biointerface Research in Applied Chemistry VL - 11 IS - 1 SP - 7932-7942 EP - 7932-7942 PB - AMG Transcend Association SN - 20695837 KW - quadruplex KW - DNA amplification KW - the point of care KW - lead cations KW - fluorescence UR - https://doi.org/10.33263/BRIAC111.79327942 L2 - https://doi.org/10.33263/BRIAC111.79327942 N2 - Quadruplex priming amplification (QPA) is a very simple amplification assay in which the isothermal amplification is performed using an only DNA polymerase, and detection is conducted by the intrinsic fluorescence of the primers. QPA employs specific G-rich sequences as primers that, upon polymerase elongation at specific temperatures, spontaneously dissociate from the primer binding sites (PBS) and fold into a monomolecular quadruplex. Fluorescent nucleotide analogs, such as 3-methyl isoxanthopterin (3MI), when incorporated into these primers, emit light upon a quadruplex formation and permit simple, specific, and sensitive quantification without the attachment of probe molecules. Previously has developed QPA assays with truncated targets and potassium cations that demonstrate an optimal amplification around 55 degrees C. Here, we designed QPA assays with truncated target and led cations at a range of human body temperature. Lead cations reveal the most rapid amplification than potassium and strontium cations. QPA can be applied as a method for the implementation of simple and cheap diagnostics (point of care (POC)), as well as development at a range of 35-45 degrees C temperature, which will make this method more convenient for using it in molecular diagnostics. ER -
GVARJALADZE, David, Tamari GULUA, Shota GOGICHAISHVILI, Jozef HRITZ and Nunu METREVEL. Quadruplex Priming Amplification at a Range of Human Body Temperature. \textit{Biointerface Research in Applied Chemistry}. Bucuresti: AMG Transcend Association, 2021, vol.~11, No~1, p.~7932-7942. ISSN~2069-5837. Available from: https://dx.doi.org/10.33263/BRIAC111.79327942.
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