Maintenance of Aquatic Hyphomycete Cultures

C 2020

Maintenance of Aquatic Hyphomycete Cultures

MARVANOVÁ, Ludmila

Basic information

Original name

Maintenance of Aquatic Hyphomycete Cultures

Authors

MARVANOVÁ, Ludmila (203 Czech Republic, guarantor, belonging to the institution)

Edition

2. vyd. Cham, Methods to Study Litter Decomposition, p. 211-222, 12 pp. 2020

Publisher

Springer

Other information

Language

English

Type of outcome

Kapitola resp. kapitoly v odborné knize

Field of Study

10612 Mycology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

printed version "print"

References:

URL

RIV identification code

RIV/00216224:14310/20:00117435

Organization unit

Faculty of Science

ISBN

978-3-030-30514-7

DOI

http://dx.doi.org/10.1007/978-3-030-30515-4_24

Keywords in English

Culture storage; Deionized water preservation; Fungal culture preservation; Fungal genetic resources; Aquatic fungi; Liquid nitrogen preservation; Pure culture databases; Pure cultures; World culture collections

Abstract

V originále

Pure cultures of fungi are very broadly used in various human activities, including medicine and various branches of industry. Moreover, some characters required for the identification of fungi decomposing leaf litter in streams can be studied only in pure culture. This chapter presents methods to keep fungal pure cultures viable and possibly genetically unchanged while avoiding contamination. The principle is to keep cultures in conditions that reduce or even suspend metabolism. The simplest and least expensive method is preservation of pieces of agar culture in small bottles with sterile deionized water at 10 °C. This approach is suitable for short-term preservation of 2-5 years. Keeping well grown cultures on agar slants flooded with mineral oil in test tubes extends the shelf life to ca. 10 years. These methods do not require special equipment. Most effective, however, is preservation by cryoconservation under suspended metabolism in a deep freezer at –80 °C, or in liquid nitrogen at up to –196 °C. Cryoconservation ensures survival for up to ca. 40 years in the case of deep freezing and practically forever in liquid nitrogen.

Citovat

MARVANOVÁ, Ludmila. Maintenance of Aquatic Hyphomycete Cultures. In Felix Bärlocher; Mark O. Gessner; Manuel A.S. Graça. Methods to Study Litter Decomposition. 2nd ed. Cham: Springer, 2020, p. 211-222. ISBN 978-3-030-30514-7. Available from: https://dx.doi.org/10.1007/978-3-030-30515-4_24.

@inbook{1712256,
   author = {Marvanová, Ludmila},
   address = {Cham},
   booktitle = {Methods to Study Litter Decomposition},
   doi = {http://dx.doi.org/10.1007/978-3-030-30515-4_24},
   edition = {2},
   editor = {Felix Bärlocher; Mark O. Gessner; Manuel A.S. Graça},
   keywords = {Culture storage; Deionized water preservation; Fungal culture preservation; Fungal genetic resources; Aquatic fungi; Liquid nitrogen preservation; Pure culture databases; Pure cultures; World culture collections},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Cham},
   isbn = {978-3-030-30514-7},
   pages = {211-222},
   publisher = {Springer},
   title = {Maintenance of Aquatic Hyphomycete Cultures},
   url = {https://doi.org/10.1007/978-3-030-30515-4_24},
   year = {2020}
}

TY  - CHAP
ID  - 1712256
AU  - Marvanová, Ludmila
PY  - 2020
TI  - Maintenance of Aquatic Hyphomycete Cultures
VL  - Neuveden
PB  - Springer
CY  - Cham
SN  - 9783030305147
KW  - Culture storage
KW  - Deionized water preservation
KW  - Fungal culture preservation
KW  - Fungal genetic resources
KW  - Aquatic fungi
KW  - Liquid nitrogen preservation
KW  - Pure culture databases
KW  - Pure cultures
KW  - World culture collections
UR  - https://doi.org/10.1007/978-3-030-30515-4_24
L2  - https://doi.org/10.1007/978-3-030-30515-4_24
N2  - Pure cultures of fungi are very broadly used in various human activities, including medicine and various branches of industry. Moreover, some characters required for the identification of fungi decomposing leaf litter in streams can be studied only in pure culture. This chapter presents methods to keep fungal pure cultures viable and possibly genetically unchanged while avoiding contamination. The principle is to keep cultures in conditions that reduce or even suspend metabolism. The simplest and least expensive method is preservation of pieces of agar culture in small bottles with sterile deionized water at 10 °C. This approach is suitable for short-term preservation of 2-5 years. Keeping well grown cultures on agar slants flooded with mineral oil in test tubes extends the shelf life to ca. 10 years. These methods do not require special equipment. Most effective, however, is preservation by cryoconservation under suspended metabolism in a deep freezer at –80 °C, or in liquid nitrogen at up to –196 °C. Cryoconservation ensures survival for up to ca. 40 years in the case of deep freezing and practically forever in liquid nitrogen.
ER  -

MARVANOVÁ, Ludmila. Maintenance of Aquatic Hyphomycete Cultures. In Felix Bärlocher; Mark O. Gessner; Manuel A.S. Gra\c ca. \textit{Methods to Study Litter Decomposition}. 2nd ed. Cham: Springer, 2020, p.~211-222. ISBN~978-3-030-30514-7. Available from: https://dx.doi.org/10.1007/978-3-030-30515-4\_{}24.

Detailed Information on Publication Record