Maintenance of Aquatic Hyphomycete Cultures

MARVANOVÁ, Ludmila. Maintenance of Aquatic Hyphomycete Cultures. In Felix Bärlocher; Mark O. Gessner; Manuel A.S. Graça. Methods to Study Litter Decomposition. 2nd ed. Cham: Springer, 2020, p. 211-222. ISBN 978-3-030-30514-7. Available from: https://dx.doi.org/10.1007/978-3-030-30515-4_24.

Basic information

• Original name: Maintenance of Aquatic Hyphomycete Cultures
• Authors: MARVANOVÁ, Ludmila (203 Czech Republic, guarantor, belonging to the institution).
• Edition: 2. vyd. Cham, Methods to Study Litter Decomposition, p. 211-222, 12 pp. 2020.
• Publisher: Springer

Other information

• Original language: English
• Type of outcome: Chapter(s) of a specialized book
• Field of Study: 10612 Mycology
• Country of publisher: Switzerland
• Confidentiality degree: is not subject to a state or trade secret
• Publication form: printed version "print"
• WWW: URL
• RIV identification code: RIV/00216224:14310/20:00117435
• Organization unit: Faculty of Science
• ISBN: 978-3-030-30514-7
• Doi: http://dx.doi.org/10.1007/978-3-030-30515-4_24
• Keywords in English: Culture storage; Deionized water preservation; Fungal culture preservation; Fungal genetic resources; Aquatic fungi; Liquid nitrogen preservation; Pure culture databases; Pure cultures; World culture collections
• Tags: rivok, topvydavatel
• Tags: International impact, Reviewed

Abstract

Pure cultures of fungi are very broadly used in various human activities, including medicine and various branches of industry. Moreover, some characters required for the identification of fungi decomposing leaf litter in streams can be studied only in pure culture. This chapter presents methods to keep fungal pure cultures viable and possibly genetically unchanged while avoiding contamination. The principle is to keep cultures in conditions that reduce or even suspend metabolism. The simplest and least expensive method is preservation of pieces of agar culture in small bottles with sterile deionized water at 10 °C. This approach is suitable for short-term preservation of 2-5 years. Keeping well grown cultures on agar slants flooded with mineral oil in test tubes extends the shelf life to ca. 10 years. These methods do not require special equipment. Most effective, however, is preservation by cryoconservation under suspended metabolism in a deep freezer at –80 °C, or in liquid nitrogen at up to –196 °C. Cryoconservation ensures survival for up to ca. 40 years in the case of deep freezing and practically forever in liquid nitrogen.