Hymenobacter terrestris sp. nov. and Hymenobacter lapidiphilus
sp. nov., isolated from regoliths in Antarctica

J 2020

Hymenobacter terrestris sp. nov. and Hymenobacter lapidiphilus sp. nov., isolated from regoliths in Antarctica

SEDLÁČEK, Ivo, Roman PANTŮČEK, Michal ZEMAN, Pavla HOLOCHOVÁ, Ondrej ŠEDO et. al.

Basic information

Original name

Hymenobacter terrestris sp. nov. and Hymenobacter lapidiphilus sp. nov., isolated from regoliths in Antarctica

Authors

SEDLÁČEK, Ivo (203 Czech Republic, guarantor, belonging to the institution), Roman PANTŮČEK (203 Czech Republic, belonging to the institution), Michal ZEMAN (703 Slovakia, belonging to the institution), Pavla HOLOCHOVÁ (203 Czech Republic, belonging to the institution), Ondrej ŠEDO (203 Czech Republic, belonging to the institution), Eva STAŇKOVÁ (203 Czech Republic, belonging to the institution), Pavel ŠVEC (203 Czech Republic, belonging to the institution), Stanislava KRÁLOVÁ (703 Slovakia, belonging to the institution), Petra VÍDEŇSKÁ (203 Czech Republic, belonging to the institution), Lenka MICENKOVÁ (703 Slovakia, belonging to the institution), Sah URVASHI (356 India), Suresh KORPOLE (356 India) and Rup LAL (356 India)

Edition

International Journal of Systematic and Evolutionary Microbiology, London, Microbiology Society, 2020, 1466-5026

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.747

RIV identification code

RIV/00216224:14310/20:00117439

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1099/ijsem.0.004540

UT WoS

000608418900041

Keywords in English

Hymenobacter; regoliths; taxonomy; Antarctica

Abstract

V originále

A group of four psychrotrophic bacterial strains was isolated on James Ross Island (Antarctica) in 2013. All isolates, originating from different soil samples, were collected from the ice-free northern part of the island. They were rod-shaped, Gram-stain-negative, and produced moderately slimy red-pink pigmented colonies on R2A agar. A polyphasic taxonomic approach based on 16S rRNA gene sequencing, whole-genome sequencing, MALDI-TOF MS, rep-PCR analyses, chemotaxonomic methods and extensive biotyping was used to clarify the taxonomic position of these isolates. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates belonged to the genus Hymenobacter. The closest relative was Hymenobacter humicola CCM 8763T, exhibiting 98.3 and 98.9% 16S rRNA pairwise similarity with the reference isolates P5342T and P5252T, respectively. Average nucleotide identity, digital DNA–DNA hybridization and core gene distances calculated from the whole-genome sequencing data confirmed that P5252T and P5342T represent two distinct Hymenobacter species. The menaquinone systems of both strains contained MK-7 as the major respiratory quinone. The predominant polar lipids for both strains were phosphatidylethanolamine and one unidentified glycolipid. The major components in the cellular fatty acid composition were summed feature 3, summed feature 4 (anteiso-C17:1 B/iso-C17:1 I), anteiso-C15:0 and iso-C15 : 0 for all isolates. Based on the obtained results, two novel species are proposed, for which the names Hymenobacter terrestris sp. nov. (type strain P5252T=CCM 8765T=LMG 31495T) and Hymenobacter lapidiphilus sp. nov. (type strain P5342T=CCM 8764T=LMG 30613T) are suggested.

Links

EF16_013/0001761, research and development project

Name: RECETOX RI

EF17_043/0009632, research and development project

Name: CETOCOEN Excellence

LM2015051, research and development project

Name: Centrum pro výzkum toxických látek v prostředí (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR

LM2015078, research and development project

Name: Česká polární výzkumná infrastruktura (Acronym: CzechPolar2)

Investor: Ministry of Education, Youth and Sports of the CR

LM2018121, research and development project

Name: Výzkumná infrastruktura RECETOX (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR, RECETOX RI

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/1127/2019, interní kód MU

Name: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 8 (Acronym: MBG 8)

Investor: Masaryk University, Category A

90127, large research infrastructures

Name: CIISB II

Citovat

SEDLÁČEK, Ivo, Roman PANTŮČEK, Michal ZEMAN, Pavla HOLOCHOVÁ, Ondrej ŠEDO, Eva STAŇKOVÁ, Pavel ŠVEC, Stanislava KRÁLOVÁ, Petra VÍDEŇSKÁ, Lenka MICENKOVÁ, Sah URVASHI, Suresh KORPOLE and Rup LAL. Hymenobacter terrestris sp. nov. and Hymenobacter lapidiphilus sp. nov., isolated from regoliths in Antarctica. International Journal of Systematic and Evolutionary Microbiology. London: Microbiology Society, 2020, vol. 70, No 12, p. 6364-6372. ISSN 1466-5026. Available from: https://dx.doi.org/10.1099/ijsem.0.004540.

@article{1712336,
   author = {Sedláček, Ivo and Pantůček, Roman and Zeman, Michal and Holochová, Pavla and Šedo, Ondrej and Staňková, Eva and Švec, Pavel and Králová, Stanislava and Vídeňská, Petra and Micenková, Lenka and Urvashi, Sah and Korpole, Suresh and Lal, Rup},
   article_location = {London},
   article_number = {12},
   doi = {http://dx.doi.org/10.1099/ijsem.0.004540},
   keywords = {Hymenobacter; regoliths; taxonomy; Antarctica},
   language = {eng},
   issn = {1466-5026},
   journal = {International Journal of Systematic and Evolutionary Microbiology},
   note = {CZ.02.1.01/0.0/0.0/18_046/0015975 neuplatňovat. Místo toho uplatnit EF17_043/0009632.},
   title = {Hymenobacter terrestris sp. nov. and Hymenobacter lapidiphilus sp. nov., isolated from regoliths in Antarctica},
   url = {https://doi.org/10.1099/ijsem.0.004540},
   volume = {70},
   year = {2020}
}

TY  - JOUR
ID  - 1712336
AU  - Sedláček, Ivo - Pantůček, Roman - Zeman, Michal - Holochová, Pavla - Šedo, Ondrej - Staňková, Eva - Švec, Pavel - Králová, Stanislava - Vídeňská, Petra - Micenková, Lenka - Urvashi, Sah - Korpole, Suresh - Lal, Rup
PY  - 2020
TI  - Hymenobacter terrestris sp. nov. and Hymenobacter lapidiphilus sp. nov., isolated from regoliths in Antarctica
JF  - International Journal of Systematic and Evolutionary Microbiology
VL  - 70
IS  - 12
SP  - 6364-6372
EP  - 6364-6372
PB  - Microbiology Society
SN  - 14665026
N1  - CZ.02.1.01/0.0/0.0/18_046/0015975 neuplatňovat. Místo toho uplatnit EF17_043/0009632.
KW  - Hymenobacter
KW  - regoliths
KW  - taxonomy
KW  - Antarctica
UR  - https://doi.org/10.1099/ijsem.0.004540
L2  - https://doi.org/10.1099/ijsem.0.004540
N2  - A group of four psychrotrophic bacterial strains was isolated on James Ross Island (Antarctica) in 2013. All isolates, originating from different soil samples, were collected from the ice-free northern part of the island. They were rod-shaped, Gram-stain-negative, and produced moderately slimy red-pink pigmented colonies on R2A agar. A polyphasic taxonomic approach based on 16S rRNA gene sequencing, whole-genome sequencing, MALDI-TOF MS, rep-PCR analyses, chemotaxonomic methods and extensive biotyping was used to clarify the taxonomic position of these isolates. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolates belonged to the genus Hymenobacter. The closest relative was Hymenobacter humicola CCM 8763T, exhibiting 98.3 and 98.9% 16S rRNA pairwise similarity with the reference isolates P5342T and P5252T, respectively. Average nucleotide identity, digital DNA–DNA hybridization and core gene distances calculated from the whole-genome sequencing data confirmed that P5252T and P5342T represent two distinct Hymenobacter species. The menaquinone systems of both strains contained MK-7 as the major respiratory quinone. The predominant polar lipids for both strains were phosphatidylethanolamine and one unidentified glycolipid. The major components in the cellular fatty acid composition were summed feature 3, summed feature 4 (anteiso-C17:1 B/iso-C17:1 I), anteiso-C15:0 and iso-C15 : 0 for all isolates. Based on the obtained results, two novel species are proposed, for which the names Hymenobacter terrestris sp. nov. (type strain P5252T=CCM 8765T=LMG 31495T) and Hymenobacter lapidiphilus sp. nov. (type strain P5342T=CCM 8764T=LMG 30613T) are suggested.
ER  -

SEDLÁČEK, Ivo, Roman PANTŮČEK, Michal ZEMAN, Pavla HOLOCHOVÁ, Ondrej ŠEDO, Eva STAŇKOVÁ, Pavel ŠVEC, Stanislava KRÁLOVÁ, Petra VÍDEŇSKÁ, Lenka MICENKOVÁ, Sah URVASHI, Suresh KORPOLE and Rup LAL. Hymenobacter terrestris sp. nov. and Hymenobacter lapidiphilus sp. nov., isolated from regoliths in Antarctica. \textit{International Journal of Systematic and Evolutionary Microbiology}. London: Microbiology Society, 2020, vol.~70, No~12, p.~6364-6372. ISSN~1466-5026. Available from: https://dx.doi.org/10.1099/ijsem.0.004540.

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