| SERNA, I. M. R., I. ROMITO, A. MAUGERI, Oriana LO RE, Sebastiano GIALLONGO, G. MAZZOCCOLI, J. A. OBEN, G. LI VOLTI, T. MAZZA, A. ALISI and Manlio VINCIGUERRA. A Lipidomic Signature Complements Stemness Features Acquisition in Liver Cancer Cells. International Journal of Molecular Sciences. Basel: Multidisciplinary Digital Publishing Institute, 2020, vol. 21, No 22, p. 1-17. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms21228452. |
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@article{1713476,
author = {Serna, I. M. R. and Romito, I. and Maugeri, A. and Lo Re, Oriana and Giallongo, Sebastiano and Mazzoccoli, G. and Oben, J. A. and Li Volti, G. and Mazza, T. and Alisi, A. and Vinciguerra, Manlio},
article_location = {Basel},
article_number = {22},
doi = {http://dx.doi.org/10.3390/ijms21228452},
keywords = {FAK; HCC; stemness; cancer stem cells},
language = {eng},
issn = {1422-0067},
journal = {International Journal of Molecular Sciences},
title = {A Lipidomic Signature Complements Stemness Features Acquisition in Liver Cancer Cells},
url = {https://www.mdpi.com/1422-0067/21/22/8452/htm},
volume = {21},
year = {2020}
}
TY - JOUR
ID - 1713476
AU - Serna, I. M. R. - Romito, I. - Maugeri, A. - Lo Re, Oriana - Giallongo, Sebastiano - Mazzoccoli, G. - Oben, J. A. - Li Volti, G. - Mazza, T. - Alisi, A. - Vinciguerra, Manlio
PY - 2020
TI - A Lipidomic Signature Complements Stemness Features Acquisition in Liver Cancer Cells
JF - International Journal of Molecular Sciences
VL - 21
IS - 22
SP - 1-17
EP - 1-17
PB - Multidisciplinary Digital Publishing Institute
SN - 14220067
KW - FAK
KW - HCC
KW - stemness
KW - cancer stem cells
UR - https://www.mdpi.com/1422-0067/21/22/8452/htm
L2 - https://www.mdpi.com/1422-0067/21/22/8452/htm
N2 - Lipid catabolism and anabolism changes play a role in stemness acquisition by cancer cells, and cancer stem cells (CSCs) are particularly dependent on the activity of the enzymes involved in these processes. Lipidomic changes could play a role in CSCs' ability to cause disease relapse and chemoresistance. The exploration of lipid composition and metabolism changes in CSCs in the context of hepatocellular cancer (HCC) is still incomplete and their lipidomic scenario continues to be elusive. We aimed to evaluate through high-throughput mass spectrometry (MS)-based lipidomics the levels of the members of the six major classes of sphingolipids and phospholipids in two HCC cell lines (HepG2 and Huh-7) silenced for the expression of histone variant macroH2A1 (favoring stemness acquisition), or silenced for the expression of focal adhesion tyrosine kinase (FAK) (hindering aggressiveness and stemness). Transcriptomic changes were evaluated by RNA sequencing as well. We found definite lipidomic and transcriptomic changes in the HCC lines upon knockdown (KD) of macroH2A1 or FAK, in line with the acquisition or loss of stemness features. In particular, macroH2A1 KD increased total sphingomyelin (SM) levels and decreased total lysophosphatidylcholine (LPC) levels, while FAK KD decreased total phosphatidylcholine (PC) levels. In conclusion, in HCC cell lines knocked down for specific signaling/epigenetic processes driving opposite stemness potential, we defined a lipidomic signature that hallmarks hepatic CSCs to be exploited for therapeutic strategies.
ER -
SERNA, I. M. R., I. ROMITO, A. MAUGERI, Oriana LO RE, Sebastiano GIALLONGO, G. MAZZOCCOLI, J. A. OBEN, G. LI VOLTI, T. MAZZA, A. ALISI and Manlio VINCIGUERRA. A Lipidomic Signature Complements Stemness Features Acquisition in Liver Cancer Cells. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, 2020, vol.~21, No~22, p.~1-17. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms21228452.
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