| MOTIEI, Marjan, Katerina VACULIKOVA, Andrea CELÁ, Katerina TVRDONOVA, Reza KHALILI, David RUMPIK, Tatana RUMPIKOVA, Zdeněk GLATZ and Tomas SAHA. Non-Invasive Human Embryo Metabolic Assessment as a Developmental Criterion. Journal of Clinical Medicine. Basel: MDPI, 2020, vol. 9, No 12, p. 4094-4106. ISSN 2077-0383. Available from: https://dx.doi.org/10.3390/jcm9124094. |
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@article{1715176,
author = {Motiei, Marjan and Vaculikova, Katerina and Celá, Andrea and Tvrdonova, Katerina and Khalili, Reza and Rumpik, David and Rumpikova, Tatana and Glatz, Zdeněk and Saha, Tomas},
article_location = {Basel},
article_number = {12},
doi = {http://dx.doi.org/10.3390/jcm9124094},
keywords = {IVF; capillary electrophoresis; metabolomics},
language = {eng},
issn = {2077-0383},
journal = {Journal of Clinical Medicine},
title = {Non-Invasive Human Embryo Metabolic Assessment as a Developmental Criterion},
url = {https://www.mdpi.com/2077-0383/9/12/4094?utm_source=releaseissue&utm_medium=email&utm_campaign=releaseissue_jcm&utm_term=titlelink199},
volume = {9},
year = {2020}
}
TY - JOUR
ID - 1715176
AU - Motiei, Marjan - Vaculikova, Katerina - Celá, Andrea - Tvrdonova, Katerina - Khalili, Reza - Rumpik, David - Rumpikova, Tatana - Glatz, Zdeněk - Saha, Tomas
PY - 2020
TI - Non-Invasive Human Embryo Metabolic Assessment as a Developmental Criterion
JF - Journal of Clinical Medicine
VL - 9
IS - 12
SP - 4094-4106
EP - 4094-4106
PB - MDPI
SN - 20770383
KW - IVF
KW - capillary electrophoresis
KW - metabolomics
UR - https://www.mdpi.com/2077-0383/9/12/4094?utm_source=releaseissue&utm_medium=email&utm_campaign=releaseissue_jcm&utm_term=titlelink199
N2 - The selection of a highly-viable single embryo in assisted reproductive technology requires an acceptable predictive method in order to reduce the multiple pregnancy rate and increase the success rate. In this study, the metabolomic profiling of growing and impaired embryos was assessed on the fifth day of fertilization using capillary electrophoresis in order to find a relationship between the profiling and embryo development, and then to provide a mechanistic insight into the appearance/depletion of the metabolites. This unique qualitative technique exhibited the appearance of most non-essential amino acids and lactate, and depleting the serine, alanyl-glutamine and pyruvate in such a manner that the embryos impaired in their development secreted a considerably higher level of lactate and consumed a significantly higher amount of alanyl-glutamine. The different significant ratios of metabolomic depletion/appearance between the embryos confirm their potential for the improvement of the prospective selection of the developed single embryos, and also suggest the fact that pyruvate and alanyl-glutamine are the most critical ATP suppliers on the fifth day of blastocyst development.
ER -
MOTIEI, Marjan, Katerina VACULIKOVA, Andrea CELÁ, Katerina TVRDONOVA, Reza KHALILI, David RUMPIK, Tatana RUMPIKOVA, Zdeněk GLATZ and Tomas SAHA. Non-Invasive Human Embryo Metabolic Assessment as a Developmental Criterion. \textit{Journal of Clinical Medicine}. Basel: MDPI, 2020, vol.~9, No~12, p.~4094-4106. ISSN~2077-0383. Available from: https://dx.doi.org/10.3390/jcm9124094.
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