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@article{1718816,
author = {Sumbal, Jakub and Budková, Zuzana and Traustadottir, Gunnhildur Asta and Koledová, Zuzana},
article_location = {New York (NY, USA)},
article_number = {4},
doi = {http://dx.doi.org/10.1007/s10911-020-09468-x},
keywords = {3D cell culture; Breast; Co-culture; Extracellular matrix; Imaging; Microenvironment; Organoid; Screening; Stromal cells},
language = {eng},
issn = {1083-3021},
journal = {Journal of Mammary Gland Biology and Neoplasia},
title = {Mammary Organoids and 3D Cell Cultures: Old Dogs with New Tricks},
url = {https://link.springer.com/article/10.1007%2Fs10911-020-09468-x},
volume = {25},
year = {2020}
}
TY - JOUR
ID - 1718816
AU - Sumbal, Jakub - Budková, Zuzana - Traustadottir, Gunnhildur Asta - Koledová, Zuzana
PY - 2020
TI - Mammary Organoids and 3D Cell Cultures: Old Dogs with New Tricks
JF - Journal of Mammary Gland Biology and Neoplasia
VL - 25
IS - 4
SP - 273-288
EP - 273-288
PB - SPRINGER/PLENUM PUBLISHERS
SN - 10833021
KW - 3D cell culture
KW - Breast
KW - Co-culture
KW - Extracellular matrix
KW - Imaging
KW - Microenvironment
KW - Organoid
KW - Screening
KW - Stromal cells
UR - https://link.springer.com/article/10.1007%2Fs10911-020-09468-x
L2 - https://link.springer.com/article/10.1007%2Fs10911-020-09468-x
N2 - 3D cell culture methods have been an integral part of and an essential tool for mammary gland and breast cancer research for half a century. In fact, mammary gland researchers, who discovered and deciphered the instructive role of extracellular matrix (ECM) in mammary epithelial cell functional differentiation and morphogenesis, were the pioneers of the 3D cell culture techniques, including organoid cultures. The last decade has brought a tremendous increase in the 3D cell culture techniques, including modifications and innovations of the existing techniques, novel biomaterials and matrices, new technological approaches, and increase in 3D culture complexity, accompanied by several redefinitions of the terms "3D cell culture" and "organoid". In this review, we provide an overview of the 3D cell culture and organoid techniques used in mammary gland biology and breast cancer research. We discuss their advantages, shortcomings and current challenges, highlight the recent progress in reconstructing the complex mammary gland microenvironment in vitro and ex vivo, and identify the missing 3D cell cultures, urgently needed to aid our understanding of mammary gland development, function, physiology, and disease, including breast cancer.
ER -
SUMBAL, Jakub, Zuzana BUDKOVÁ, Gunnhildur Asta TRAUSTADOTTIR and Zuzana KOLEDOVÁ. Mammary Organoids and 3D Cell Cultures: Old Dogs with New Tricks. \textit{Journal of Mammary Gland Biology and Neoplasia}. New York (NY, USA): SPRINGER/PLENUM PUBLISHERS, 2020, vol.~25, No~4, p.~273-288. ISSN~1083-3021. Available from: https://dx.doi.org/10.1007/s10911-020-09468-x.
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