Mammary Organoids and 3D Cell Cultures: Old Dogs with New
Tricks

J 2020

Mammary Organoids and 3D Cell Cultures: Old Dogs with New Tricks

SUMBAL, Jakub, Zuzana BUDKOVÁ, Gunnhildur Asta TRAUSTADOTTIR and Zuzana KOLEDOVÁ

Basic information

Original name

Mammary Organoids and 3D Cell Cultures: Old Dogs with New Tricks

Authors

SUMBAL, Jakub (203 Czech Republic, belonging to the institution), Zuzana BUDKOVÁ (203 Czech Republic), Gunnhildur Asta TRAUSTADOTTIR (352 Iceland) and Zuzana KOLEDOVÁ (703 Slovakia, guarantor, belonging to the institution)

Edition

Journal of Mammary Gland Biology and Neoplasia, New York (NY, USA), SPRINGER/PLENUM PUBLISHERS, 2020, 1083-3021

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30202 Endocrinology and metabolism

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.673

RIV identification code

RIV/00216224:14110/20:00117550

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1007/s10911-020-09468-x

UT WoS

000590496200001

Keywords in English

3D cell culture; Breast; Co-culture; Extracellular matrix; Imaging; Microenvironment; Organoid; Screening; Stromal cells

Abstract

V originále

3D cell culture methods have been an integral part of and an essential tool for mammary gland and breast cancer research for half a century. In fact, mammary gland researchers, who discovered and deciphered the instructive role of extracellular matrix (ECM) in mammary epithelial cell functional differentiation and morphogenesis, were the pioneers of the 3D cell culture techniques, including organoid cultures. The last decade has brought a tremendous increase in the 3D cell culture techniques, including modifications and innovations of the existing techniques, novel biomaterials and matrices, new technological approaches, and increase in 3D culture complexity, accompanied by several redefinitions of the terms "3D cell culture" and "organoid". In this review, we provide an overview of the 3D cell culture and organoid techniques used in mammary gland biology and breast cancer research. We discuss their advantages, shortcomings and current challenges, highlight the recent progress in reconstructing the complex mammary gland microenvironment in vitro and ex vivo, and identify the missing 3D cell cultures, urgently needed to aid our understanding of mammary gland development, function, physiology, and disease, including breast cancer.

Links

MUNI/A/1382/2019, interní kód MU

Name: Zdroje pro tkáňové inženýrství 10 (Acronym: TissueEng 10)

Investor: Masaryk University, Category A

MUNI/G/1446/2018, interní kód MU

Name: Deciphering the mechanisms of mammary epithelial branched pattern formation through iterative biological and mathematical modelling

Investor: Masaryk University, INTERDISCIPLINARY - Interdisciplinary research projects

ROZV/28/LF19/2020, interní kód MU

Name: Regulace morfogeneze epitelu mléčné žlázy pomocí mechanických sil a dynamiky signalizace

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

Citovat

SUMBAL, Jakub, Zuzana BUDKOVÁ, Gunnhildur Asta TRAUSTADOTTIR and Zuzana KOLEDOVÁ. Mammary Organoids and 3D Cell Cultures: Old Dogs with New Tricks. Journal of Mammary Gland Biology and Neoplasia. New York (NY, USA): SPRINGER/PLENUM PUBLISHERS, 2020, vol. 25, No 4, p. 273-288. ISSN 1083-3021. Available from: https://dx.doi.org/10.1007/s10911-020-09468-x.

@article{1718816,
   author = {Sumbal, Jakub and Budková, Zuzana and Traustadottir, Gunnhildur Asta and Koledová, Zuzana},
   article_location = {New York (NY, USA)},
   article_number = {4},
   doi = {http://dx.doi.org/10.1007/s10911-020-09468-x},
   keywords = {3D cell culture; Breast; Co-culture; Extracellular matrix; Imaging; Microenvironment; Organoid; Screening; Stromal cells},
   language = {eng},
   issn = {1083-3021},
   journal = {Journal of Mammary Gland Biology and Neoplasia},
   title = {Mammary Organoids and 3D Cell Cultures: Old Dogs with New Tricks},
   url = {https://link.springer.com/article/10.1007%2Fs10911-020-09468-x},
   volume = {25},
   year = {2020}
}

TY  - JOUR
ID  - 1718816
AU  - Sumbal, Jakub - Budková, Zuzana - Traustadottir, Gunnhildur Asta - Koledová, Zuzana
PY  - 2020
TI  - Mammary Organoids and 3D Cell Cultures: Old Dogs with New Tricks
JF  - Journal of Mammary Gland Biology and Neoplasia
VL  - 25
IS  - 4
SP  - 273-288
EP  - 273-288
PB  - SPRINGER/PLENUM PUBLISHERS
SN  - 10833021
KW  - 3D cell culture
KW  - Breast
KW  - Co-culture
KW  - Extracellular matrix
KW  - Imaging
KW  - Microenvironment
KW  - Organoid
KW  - Screening
KW  - Stromal cells
UR  - https://link.springer.com/article/10.1007%2Fs10911-020-09468-x
L2  - https://link.springer.com/article/10.1007%2Fs10911-020-09468-x
N2  - 3D cell culture methods have been an integral part of and an essential tool for mammary gland and breast cancer research for half a century. In fact, mammary gland researchers, who discovered and deciphered the instructive role of extracellular matrix (ECM) in mammary epithelial cell functional differentiation and morphogenesis, were the pioneers of the 3D cell culture techniques, including organoid cultures. The last decade has brought a tremendous increase in the 3D cell culture techniques, including modifications and innovations of the existing techniques, novel biomaterials and matrices, new technological approaches, and increase in 3D culture complexity, accompanied by several redefinitions of the terms "3D cell culture" and "organoid". In this review, we provide an overview of the 3D cell culture and organoid techniques used in mammary gland biology and breast cancer research. We discuss their advantages, shortcomings and current challenges, highlight the recent progress in reconstructing the complex mammary gland microenvironment in vitro and ex vivo, and identify the missing 3D cell cultures, urgently needed to aid our understanding of mammary gland development, function, physiology, and disease, including breast cancer.
ER  -

SUMBAL, Jakub, Zuzana BUDKOVÁ, Gunnhildur Asta TRAUSTADOTTIR and Zuzana KOLEDOVÁ. Mammary Organoids and 3D Cell Cultures: Old Dogs with New Tricks. \textit{Journal of Mammary Gland Biology and Neoplasia}. New York (NY, USA): SPRINGER/PLENUM PUBLISHERS, 2020, vol.~25, No~4, p.~273-288. ISSN~1083-3021. Available from: https://dx.doi.org/10.1007/s10911-020-09468-x.

Detailed Information on Publication Record