Subarachnoid Hemorrhage Increases Level of Heme Oxygenase-1 and
Biliverdin Reductase in the Choroid Plexus

J 2020

Subarachnoid Hemorrhage Increases Level of Heme Oxygenase-1 and Biliverdin Reductase in the Choroid Plexus

SOLÁR, Peter, Václav BRÁZDA, Shahaf LEVIN, Alemeh ZAMANI, Radim JANČÁLEK et. al.

Basic information

Original name

Subarachnoid Hemorrhage Increases Level of Heme Oxygenase-1 and Biliverdin Reductase in the Choroid Plexus

Authors

SOLÁR, Peter (703 Slovakia, belonging to the institution), Václav BRÁZDA (203 Czech Republic, belonging to the institution), Shahaf LEVIN (376 Israel, belonging to the institution), Alemeh ZAMANI (364 Islamic Republic of Iran, belonging to the institution), Radim JANČÁLEK (203 Czech Republic, belonging to the institution), Petr DUBOVÝ (203 Czech Republic, belonging to the institution) and Marek JOUKAL (203 Czech Republic, guarantor, belonging to the institution)

Edition

Frontiers in Cellular Neuroscience, Lausanne, Switzerland, Frontiers, 2020, 1662-5102

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30103 Neurosciences

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.505

RIV identification code

RIV/00216224:14110/20:00117551

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3389/fncel.2020.593305

UT WoS

000596842200001

Keywords in English

subarachnoid hemorrhage; choroid plexus; heme oxygenase-1; biliverdin reductase; macrophages

Abstract

V originále

Subarachnoid hemorrhage is a specific, life-threatening form of hemorrhagic stroke linked to high morbidity and mortality. It has been found that the choroid plexus of the brain ventricles forming the blood-cerebrospinal fluid barrier plays an important role in subarachnoid hemorrhage pathophysiology. Heme oxygenase-1 and biliverdin reductase are two of the key enzymes of the hemoglobin degradation cascade. Therefore, the aim of present study was to investigate changes in protein levels of heme oxygenase-1 and biliverdin reductase in the rat choroid plexus after experimental subarachnoid hemorrhage induced by injection of non-heparinized autologous blood to the cisterna magna. Artificial cerebrospinal fluid of the same volume as autologous blood was injected to mimic increased intracranial pressure in control rats. Immunohistochemical and Western blot analyses were used to monitor changes in the of heme oxygenase-1 and biliverdin reductase levels in the rat choroid plexus after induction of subarachnoid hemorrhage or artificial cerebrospinal fluid application for 1, 3, and 7 days. We found increased levels of heme oxygenase-1 and biliverdin reductase protein in the choroid plexus over the entire period following subarachnoid hemorrhage induction. The level of heme oxygenase-1 was the highest early (1 and 3 days) after subarachnoid hemorrhage indicating its importance in hemoglobin degradation. Increased levels of heme oxygenase-1 were also observed in the choroid plexus epithelial cells at all time points after application of artificial cerebrospinal fluid. Biliverdin reductase protein was detected mainly in the choroid plexus epithelial cells, with levels gradually increasing during subarachnoid hemorrhage. Our results suggest that heme oxygenase-1 and biliverdin reductase are involved not only in hemoglobin degradation but probably also in protecting choroid plexus epithelial cells and the blood-cerebrospinal fluid barrier from the negative effects of subarachnoid hemorrhage.

Links

EF16_027/0008360, research and development project

Name: Postdoc@MUNI

MUNI/A/0975/2019, interní kód MU

Name: Studium změn ve strukturách nervové soustavy po poškození

Investor: Masaryk University, Category A

MUNI/A/1086/2018, interní kód MU

Name: Reakce struktur nervové soustavy na poškození

Investor: Masaryk University, Category A

ROZV/23/LF14/2019, interní kód MU

Name: Elektrofyziologická a imunohistochemická studie změn podmíněných neurozánětem ve spinálních gangliích neasociovaných s poškozeným periferním nervem

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

ROZV/28/LF16/2020, interní kód MU

Name: Studium hematolikvorové bariéry po poškození nervové soustavy

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

Citovat

SOLÁR, Peter, Václav BRÁZDA, Shahaf LEVIN, Alemeh ZAMANI, Radim JANČÁLEK, Petr DUBOVÝ and Marek JOUKAL. Subarachnoid Hemorrhage Increases Level of Heme Oxygenase-1 and Biliverdin Reductase in the Choroid Plexus. Frontiers in Cellular Neuroscience. Lausanne, Switzerland: Frontiers, 2020, vol. 14, NOV 2020, p. 1-11. ISSN 1662-5102. Available from: https://dx.doi.org/10.3389/fncel.2020.593305.

@article{1718836,
   author = {Solár, Peter and Brázda, Václav and Levin, Shahaf and Zamani, Alemeh and Jančálek, Radim and Dubový, Petr and Joukal, Marek},
   article_location = {Lausanne, Switzerland},
   article_number = {NOV 2020},
   doi = {http://dx.doi.org/10.3389/fncel.2020.593305},
   keywords = {subarachnoid hemorrhage; choroid plexus; heme oxygenase-1; biliverdin reductase; macrophages},
   language = {eng},
   issn = {1662-5102},
   journal = {Frontiers in Cellular Neuroscience},
   title = {Subarachnoid Hemorrhage Increases Level of Heme Oxygenase-1 and Biliverdin Reductase in the Choroid Plexus},
   url = {https://www.frontiersin.org/articles/10.3389/fncel.2020.593305/full},
   volume = {14},
   year = {2020}
}

TY  - JOUR
ID  - 1718836
AU  - Solár, Peter - Brázda, Václav - Levin, Shahaf - Zamani, Alemeh - Jančálek, Radim - Dubový, Petr - Joukal, Marek
PY  - 2020
TI  - Subarachnoid Hemorrhage Increases Level of Heme Oxygenase-1 and Biliverdin Reductase in the Choroid Plexus
JF  - Frontiers in Cellular Neuroscience
VL  - 14
IS  - NOV 2020
SP  - 1-11
EP  - 1-11
PB  - Frontiers
SN  - 16625102
KW  - subarachnoid hemorrhage
KW  - choroid plexus
KW  - heme oxygenase-1
KW  - biliverdin reductase
KW  - macrophages
UR  - https://www.frontiersin.org/articles/10.3389/fncel.2020.593305/full
L2  - https://www.frontiersin.org/articles/10.3389/fncel.2020.593305/full
N2  - Subarachnoid hemorrhage is a specific, life-threatening form of hemorrhagic stroke linked to high morbidity and mortality. It has been found that the choroid plexus of the brain ventricles forming the blood-cerebrospinal fluid barrier plays an important role in subarachnoid hemorrhage pathophysiology. Heme oxygenase-1 and biliverdin reductase are two of the key enzymes of the hemoglobin degradation cascade. Therefore, the aim of present study was to investigate changes in protein levels of heme oxygenase-1 and biliverdin reductase in the rat choroid plexus after experimental subarachnoid hemorrhage induced by injection of non-heparinized autologous blood to the cisterna magna. Artificial cerebrospinal fluid of the same volume as autologous blood was injected to mimic increased intracranial pressure in control rats. Immunohistochemical and Western blot analyses were used to monitor changes in the of heme oxygenase-1 and biliverdin reductase levels in the rat choroid plexus after induction of subarachnoid hemorrhage or artificial cerebrospinal fluid application for 1, 3, and 7 days. We found increased levels of heme oxygenase-1 and biliverdin reductase protein in the choroid plexus over the entire period following subarachnoid hemorrhage induction. The level of heme oxygenase-1 was the highest early (1 and 3 days) after subarachnoid hemorrhage indicating its importance in hemoglobin degradation. Increased levels of heme oxygenase-1 were also observed in the choroid plexus epithelial cells at all time points after application of artificial cerebrospinal fluid. Biliverdin reductase protein was detected mainly in the choroid plexus epithelial cells, with levels gradually increasing during subarachnoid hemorrhage. Our results suggest that heme oxygenase-1 and biliverdin reductase are involved not only in hemoglobin degradation but probably also in protecting choroid plexus epithelial cells and the blood-cerebrospinal fluid barrier from the negative effects of subarachnoid hemorrhage.
ER  -

SOLÁR, Peter, Václav BRÁZDA, Shahaf LEVIN, Alemeh ZAMANI, Radim JANČÁLEK, Petr DUBOVÝ and Marek JOUKAL. Subarachnoid Hemorrhage Increases Level of Heme Oxygenase-1 and Biliverdin Reductase in the Choroid Plexus. \textit{Frontiers in Cellular Neuroscience}. Lausanne, Switzerland: Frontiers, 2020, vol.~14, NOV 2020, p.~1-11. ISSN~1662-5102. Available from: https://dx.doi.org/10.3389/fncel.2020.593305.

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