Detailed Information on Publication Record
2020
Comparison of Dietary Oils with Different Polyunsaturated Fatty Acid n-3 and n-6 Content in the Rat Model of Cutaneous Wound Healing
KOMPRDA, Tomáš, Zbyšek SLÁDEK, Zuzana ŠEVČÍKOVÁ, Veronika ŠVEHLOVÁ, JanJ WIJACKI et. al.Basic information
Original name
Comparison of Dietary Oils with Different Polyunsaturated Fatty Acid n-3 and n-6 Content in the Rat Model of Cutaneous Wound Healing
Authors
KOMPRDA, Tomáš, Zbyšek SLÁDEK, Zuzana ŠEVČÍKOVÁ, Veronika ŠVEHLOVÁ, JanJ WIJACKI, Roman GURÁŇ, TTomáš DO, Zuzana LACKOVÁ, Hana HOLCOVÁ POLANSKÁ (203 Czech Republic, belonging to the institution), Lucie VRLÍKOVÁ, Vendula POPELKOVÁ, Petr MICHÁLEK, Ondřej ZITKA and Marcela BUCHTOVÁ (203 Czech Republic, belonging to the institution)
Edition
International Journal of Molecular Sciences, Basel, MDPI, 2020, 1422-0067
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10608 Biochemistry and molecular biology
Country of publisher
Switzerland
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 5.923
RIV identification code
RIV/00216224:14310/20:00117555
Organization unit
Faculty of Science
UT WoS
000588974300001
Keywords in English
polyunsaturated fatty acids; hydroxyproline; collagen I; III; MPO; macrophages; mast cells; matrix-assisted-laser-desorption-ionization-mass-spectrometry-imaging
Tags
Tags
International impact, Reviewed
Změněno: 17/2/2023 12:22, Mgr. Marie Šípková, DiS.
Abstract
V originále
Dietary supplementation with polyunsaturated fatty acids (PUFA) n-3 can affect cutaneous wound healing; however, recent findings demonstrate the variable extent of their influence on the quality of healing. Here, we compare the effect of several dietary oils, containing different levels of PUFA n-3 and PUFA n-6, on wound healing in the rat model. Rats were fed the feed mixture with 8% palm oil (P), safflower oil (S), fish oil (F) or Schizochytrium microalga extract (Sch) and compared to the animals fed by control feed mixture (C). Dorsal full-thickness cutaneous excisions were performed after 52 days of feeding and skin was left to heal for an additional 12 days. Histopathological analysis of skin wounds was performed, including immune cells immunolabeling and the determination of hydroxyproline amount as well as gene expression analyses of molecules contributing to different steps of the healing. Matrix-assisted-laser-desorption-ionization mass-spectrometry-imaging (MALDI-MSI) was used to determine the amount of collagen alpha-1(III) chain fragment in healing samples. Treatment by Schizochytrium extract resulted in decrease in the total wound area, in contrast to the safflower oil group where the size of the wound was larger when comparing to control animals. Diet with Schizochytrium extract and safflower oils displayed a tendency to increase the number of new vessels. The number of MPO-positive cells was diminished following any of oil treatment in comparison to the control, but their highest amount was found in animals with a fish oil diet. On the other hand, the number of CD68-positive macrophages was increased, with the most significant enhancement in the fish oil and safflower oil group. Hydroxyproline concentration was the highest in the safflower oil group but it was also enhanced in all other analyzed treatments in comparison to the control. MALDI-MSI signal intensity of a collagen III fragment decreased in the sequence C > S > Sch > P > F treatment. In conclusion, we observed differences in tissue response during healing between dietary oils, with the activation of inflammation observed following the treatment with oil containing high eicosapentaenoic acid (EPA) level (fish oil) and enhanced healing features were induced by the diet with high content of docosahexaenoic acid (DHA, Schizochytrium extract).
Links
| LQ1601, research and development project |
|