Early Changes in Nitrate Uptake and Assimilation Under Drought in Relation to Transpiration

@article{1720636,
   author = {Gloser, Vít and Dvořáčková, Michaela and Hernandez Mota, Daniel and Petrovic, Bojana and Gonzalez, Patricia and Geilfus, Christoph Martin},
   article_location = {Lausanne},
   article_number = {December 2020},
   doi = {http://dx.doi.org/10.3389/fpls.2020.602065},
   keywords = {nitrate transport; nitrogen deficiency; nitrate reductase; pH; xylem; apoplast},
   language = {eng},
   issn = {1664-462X},
   journal = {Frontiers in Plant Science},
   title = {Early Changes in Nitrate Uptake and Assimilation Under Drought in Relation to Transpiration},
   url = {https://doi.org/10.3389/fpls.2020.602065},
   volume = {11},
   year = {2020}
}

TY  - JOUR
ID  - 1720636
AU  - Gloser, Vít - Dvořáčková, Michaela - Hernandez Mota, Daniel - Petrovic, Bojana - Gonzalez, Patricia - Geilfus, Christoph Martin
PY  - 2020
TI  - Early Changes in Nitrate Uptake and Assimilation Under Drought in Relation to Transpiration
JF  - Frontiers in Plant Science
VL  - 11
IS  - December 2020
SP  - 1-11
EP  - 1-11
PB  - Frontiers Media
SN  - 1664462X
KW  - nitrate transport
KW  - nitrogen deficiency
KW  - nitrate reductase
KW  - pH
KW  - xylem
KW  - apoplast
UR  - https://doi.org/10.3389/fpls.2020.602065
L2  - https://doi.org/10.3389/fpls.2020.602065
N2  - Soil drying combined with nitrogen (N) deficiency poses a grave threat to agricultural crop production. The rate at which nitrate (NO3-) is taken up depends partly on the uptake and transpiration of water. Rapid changes in nitrate assimilation, in contrast to other N forms, may serve as a component of the plant stress response to drought because nitrate assimilation may lead to changes in xylem pH. The modulation of xylem sap pH may be relevant for stomata regulation via the delivery of abscisic acid (ABA) to guard cells. In several factorial experiments, we investigated the interactions between nitrate and water availability on nitrate fate in the plant, as well as their possible implications for the early drought-stress response. We monitored the short-term response (2-6 days) of nitrate in biomass, transport to shoot and reduction in Pisum sativum, Hordeum vulgare, Vicia faba, and Nicotiana tabacum and correlated this with sap pH and transpiration rates (TRs). Cultivation on inorganic substrate ensured control over nutrient and water supply and prevented nodulation in legume species. NO3- content in biomass decreased in most of the species under drought indicating significant decline in NO3- uptake. Hordeum vulgare had the highest NO3- concentrations in all organs even under drought and low NO3- treatment. This species can likely respond much better to the combined adverse effects of low NO3- and water scarcity. Nitrate reductase activity (NRA) was reduced in both roots and leaves of water deficient (WD) plants in all species except H. vulgare, presumably due to its high NO3- contents. Further, transient reduction in NO3- availability had no effect on sap pH. Therefore, it seems unlikely that NRA shifts from shoot root leading to the supposed alkalization of sap. We also did not observe any interactive effects of NO3- and water deficiency on transpiration. Hence, as long as leaf NO3- content remains stable, NO3- availability in soil is not linked to short-term modulation of transpiration.
ER  - 

GLOSER, Vít, Michaela DVOŘÁČKOVÁ, Daniel HERNANDEZ MOTA, Bojana PETROVIC, Patricia GONZALEZ and Christoph Martin GEILFUS. Early Changes in Nitrate Uptake and Assimilation Under Drought in Relation to Transpiration. \textit{Frontiers in Plant Science}. Lausanne: Frontiers Media, 2020, vol.~11, December 2020, p.~1-11. ISSN~1664-462X. Available from: https://dx.doi.org/10.3389/fpls.2020.602065.