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@article{1721716,
author = {Kahounová, Zuzana and Remšík, Ján and Fedr, Radek and Bouchal, Jan and Mičková, Alena and Slabáková, Eva and Binó, Lucia and Hampl, Aleš and Souček, Karel},
article_location = {Amsterdam},
article_number = {July 2020},
doi = {http://dx.doi.org/10.1016/j.scr.2020.101844},
keywords = {Prostate stern cells; Epithelial-to-mesenchymal transition; Snai2/Slug; Organoids; Sternness},
language = {eng},
issn = {1873-5061},
journal = {Stem cell research},
title = {Slug-expressing mouse prostate epithelial cells have increased stem cell potential},
url = {https://doi.org/10.1016/j.scr.2020.101844},
volume = {46},
year = {2020}
}
TY - JOUR
ID - 1721716
AU - Kahounová, Zuzana - Remšík, Ján - Fedr, Radek - Bouchal, Jan - Mičková, Alena - Slabáková, Eva - Binó, Lucia - Hampl, Aleš - Souček, Karel
PY - 2020
TI - Slug-expressing mouse prostate epithelial cells have increased stem cell potential
JF - Stem cell research
VL - 46
IS - July 2020
SP - 1-10
EP - 1-10
PB - Elsevier
SN - 18735061
KW - Prostate stern cells
KW - Epithelial-to-mesenchymal transition
KW - Snai2/Slug
KW - Organoids
KW - Sternness
UR - https://doi.org/10.1016/j.scr.2020.101844
L2 - https://doi.org/10.1016/j.scr.2020.101844
N2 - Deciphering the properties of adult stem cells is crucial for understanding of their role in healthy tissue and in cancer progression as well. Both stem cells and cancer stem cells have shown association with epithelial-to-mesenchymal transition (EMT) in various tissue types. Aiming to investigate the epithelial and mesenchymal phenotypic traits in adult mouse prostate, we sorted subpopulations of basal prostate stem cells (mPSCs) and assessed the expression levels of EMT regulators and markers with custom-designed gene expression array. The population of mPSCs defined by a Lin(-)/Sca-1(+) CD49f(hi)/Trop-2(+) (LSC Trop-(2+)) surface phenotype was enriched in mesenchymal markers, especially EMT master regulator Slug, encoded by the Snail gene. To further dissect the role of Slug in mPSCs, we used transgenic Snai2(tm1.1wbg) reporter mouse strain. Using this model, we confirmed the presence of mesenchymal traits and increase of organoid forming capacity in Slug(+) population of mPSCs. The Slug(+) -derived organoids comprised all prostate epithelial cell types - basal, luminal, and neuroendocrine. Collectively, these data uncover the important role of Slug expression in the physiology of mouse prostate stem cells.
ER -
KAHOUNOVÁ, Zuzana, Ján REMŠÍK, Radek FEDR, Jan BOUCHAL, Alena MIČKOVÁ, Eva SLABÁKOVÁ, Lucia BINÓ, Aleš HAMPL and Karel SOUČEK. Slug-expressing mouse prostate epithelial cells have increased stem cell potential. \textit{Stem cell research}. Amsterdam: Elsevier, 2020, vol.~46, July 2020, p.~1-10. ISSN~1873-5061. Available from: https://dx.doi.org/10.1016/j.scr.2020.101844.
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