Slug-expressing mouse prostate epithelial cells have increased
stem cell potential

J 2020

Slug-expressing mouse prostate epithelial cells have increased stem cell potential

KAHOUNOVÁ, Zuzana, Ján REMŠÍK, Radek FEDR, Jan BOUCHAL, Alena MIČKOVÁ et. al.

Basic information

Original name

Slug-expressing mouse prostate epithelial cells have increased stem cell potential

Authors

KAHOUNOVÁ, Zuzana, Ján REMŠÍK (703 Slovakia, belonging to the institution), Radek FEDR, Jan BOUCHAL, Alena MIČKOVÁ, Eva SLABÁKOVÁ, Lucia BINÓ, Aleš HAMPL (203 Czech Republic, belonging to the institution) and Karel SOUČEK (203 Czech Republic, belonging to the institution)

Edition

Stem cell research, Amsterdam, Elsevier, 2020, 1873-5061

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.020

RIV identification code

RIV/00216224:14310/20:00117616

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.scr.2020.101844

UT WoS

000566516300010

Keywords in English

Prostate stern cells; Epithelial-to-mesenchymal transition; Snai2/Slug; Organoids; Sternness

Abstract

V originále

Deciphering the properties of adult stem cells is crucial for understanding of their role in healthy tissue and in cancer progression as well. Both stem cells and cancer stem cells have shown association with epithelial-to-mesenchymal transition (EMT) in various tissue types. Aiming to investigate the epithelial and mesenchymal phenotypic traits in adult mouse prostate, we sorted subpopulations of basal prostate stem cells (mPSCs) and assessed the expression levels of EMT regulators and markers with custom-designed gene expression array. The population of mPSCs defined by a Lin(-)/Sca-1(+) CD49f(hi)/Trop-2(+) (LSC Trop-(2+)) surface phenotype was enriched in mesenchymal markers, especially EMT master regulator Slug, encoded by the Snail gene. To further dissect the role of Slug in mPSCs, we used transgenic Snai2(tm1.1wbg) reporter mouse strain. Using this model, we confirmed the presence of mesenchymal traits and increase of organoid forming capacity in Slug(+) population of mPSCs. The Slug(+) -derived organoids comprised all prostate epithelial cell types - basal, luminal, and neuroendocrine. Collectively, these data uncover the important role of Slug expression in the physiology of mouse prostate stem cells.

Links

EE2.3.20.0185, research and development project

Name: Centrum analýz a modelování tkání a orgánů

EE2.4.31.0245, research and development project

Name: OrganoNET - partnerství pro vzdělávání a výzkum v oblasti zobrazování tkání a orgánů

Citovat

KAHOUNOVÁ, Zuzana, Ján REMŠÍK, Radek FEDR, Jan BOUCHAL, Alena MIČKOVÁ, Eva SLABÁKOVÁ, Lucia BINÓ, Aleš HAMPL and Karel SOUČEK. Slug-expressing mouse prostate epithelial cells have increased stem cell potential. Stem cell research. Amsterdam: Elsevier, 2020, vol. 46, July 2020, p. 1-10. ISSN 1873-5061. Available from: https://dx.doi.org/10.1016/j.scr.2020.101844.

@article{1721716,
   author = {Kahounová, Zuzana and Remšík, Ján and Fedr, Radek and Bouchal, Jan and Mičková, Alena and Slabáková, Eva and Binó, Lucia and Hampl, Aleš and Souček, Karel},
   article_location = {Amsterdam},
   article_number = {July 2020},
   doi = {http://dx.doi.org/10.1016/j.scr.2020.101844},
   keywords = {Prostate stern cells; Epithelial-to-mesenchymal transition; Snai2/Slug; Organoids; Sternness},
   language = {eng},
   issn = {1873-5061},
   journal = {Stem cell research},
   title = {Slug-expressing mouse prostate epithelial cells have increased stem cell potential},
   url = {https://doi.org/10.1016/j.scr.2020.101844},
   volume = {46},
   year = {2020}
}

TY  - JOUR
ID  - 1721716
AU  - Kahounová, Zuzana - Remšík, Ján - Fedr, Radek - Bouchal, Jan - Mičková, Alena - Slabáková, Eva - Binó, Lucia - Hampl, Aleš - Souček, Karel
PY  - 2020
TI  - Slug-expressing mouse prostate epithelial cells have increased stem cell potential
JF  - Stem cell research
VL  - 46
IS  - July 2020
SP  - 1-10
EP  - 1-10
PB  - Elsevier
SN  - 18735061
KW  - Prostate stern cells
KW  - Epithelial-to-mesenchymal transition
KW  - Snai2/Slug
KW  - Organoids
KW  - Sternness
UR  - https://doi.org/10.1016/j.scr.2020.101844
L2  - https://doi.org/10.1016/j.scr.2020.101844
N2  - Deciphering the properties of adult stem cells is crucial for understanding of their role in healthy tissue and in cancer progression as well. Both stem cells and cancer stem cells have shown association with epithelial-to-mesenchymal transition (EMT) in various tissue types. Aiming to investigate the epithelial and mesenchymal phenotypic traits in adult mouse prostate, we sorted subpopulations of basal prostate stem cells (mPSCs) and assessed the expression levels of EMT regulators and markers with custom-designed gene expression array. The population of mPSCs defined by a Lin(-)/Sca-1(+) CD49f(hi)/Trop-2(+) (LSC Trop-(2+)) surface phenotype was enriched in mesenchymal markers, especially EMT master regulator Slug, encoded by the Snail gene. To further dissect the role of Slug in mPSCs, we used transgenic Snai2(tm1.1wbg) reporter mouse strain. Using this model, we confirmed the presence of mesenchymal traits and increase of organoid forming capacity in Slug(+) population of mPSCs. The Slug(+) -derived organoids comprised all prostate epithelial cell types - basal, luminal, and neuroendocrine. Collectively, these data uncover the important role of Slug expression in the physiology of mouse prostate stem cells.
ER  -

KAHOUNOVÁ, Zuzana, Ján REMŠÍK, Radek FEDR, Jan BOUCHAL, Alena MIČKOVÁ, Eva SLABÁKOVÁ, Lucia BINÓ, Aleš HAMPL and Karel SOUČEK. Slug-expressing mouse prostate epithelial cells have increased stem cell potential. \textit{Stem cell research}. Amsterdam: Elsevier, 2020, vol.~46, July 2020, p.~1-10. ISSN~1873-5061. Available from: https://dx.doi.org/10.1016/j.scr.2020.101844.

Detailed Information on Publication Record