DOBROVODSKÝ, Daniel, Aleš DAŇHEL, Jana MALÍKOVÁ and Miroslav FOJTA. Electrochemical detection of G-quadruplex by interaction with porphyrin ligand. Online. In Nesmerak, K. PROCEEDINGS OF THE 15TH INTERNATIONAL STUDENTS CONFERENCE MODERN ANALYTICAL CHEMISTRY. PRAGUE: CHARLES UNIV, FAC SCIENCE, 2019, p. 215-219. ISBN 978-80-7444-068-7.
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Basic information
Original name Electrochemical detection of G-quadruplex by interaction with porphyrin ligand
Authors DOBROVODSKÝ, Daniel (703 Slovakia, belonging to the institution), Aleš DAŇHEL (203 Czech Republic), Jana MALÍKOVÁ and Miroslav FOJTA.
Edition PRAGUE, PROCEEDINGS OF THE 15TH INTERNATIONAL STUDENTS CONFERENCE MODERN ANALYTICAL CHEMISTRY, p. 215-219, 5 pp. 2019.
Publisher CHARLES UNIV, FAC SCIENCE
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10406 Analytical chemistry
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
Publication form electronic version available online
RIV identification code RIV/00216224:14310/19:00117630
Organization unit Faculty of Science
ISBN 978-80-7444-068-7
UT WoS 000576787700035
Keywords in English cyclic voltammetry; G-quadruplex; hanging mercury drop electrode; porphyrins
Tags rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 8/1/2021 11:25.
Abstract
Porphyrins are well-known binders of DNA and can also specifically target guanine quadruplexes (G4). Interaction of a selected porphyrin (Cu-TMPyPP) with oligonucleotide sequences either forming or not forming G4 was studied using cyclic voltammetry at hanging mercury drop electrode. The interaction is shown to be detectable by cyclic voltammetry as potential shift of a characteristic peak of Cu-TMPyPP which is observed for G4, single stranded as well as double stranded DNA. Nevertheless, further changes of this peak resulting from application of cyclic potential scans to the studied DNA before interaction with Cu-TMPyPP can be used to clearly distinguish G4 DNA, which can be applied as analytical tool for its detection.
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