Electrochemical detection of G-quadruplex by interaction with
porphyrin ligand

D 2019

Electrochemical detection of G-quadruplex by interaction with porphyrin ligand

DOBROVODSKÝ, Daniel, Aleš DAŇHEL, Jana MALÍKOVÁ and Miroslav FOJTA

Basic information

Original name

Electrochemical detection of G-quadruplex by interaction with porphyrin ligand

Authors

DOBROVODSKÝ, Daniel (703 Slovakia, belonging to the institution), Aleš DAŇHEL (203 Czech Republic), Jana MALÍKOVÁ and Miroslav FOJTA

Edition

PRAGUE, PROCEEDINGS OF THE 15TH INTERNATIONAL STUDENTS CONFERENCE MODERN ANALYTICAL CHEMISTRY, p. 215-219, 5 pp. 2019

Publisher

CHARLES UNIV, FAC SCIENCE

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

electronic version available online

RIV identification code

RIV/00216224:14310/19:00117630

Organization unit

Faculty of Science

ISBN

978-80-7444-068-7

UT WoS

000576787700035

Keywords in English

cyclic voltammetry; G-quadruplex; hanging mercury drop electrode; porphyrins

Abstract

V originále

Porphyrins are well-known binders of DNA and can also specifically target guanine quadruplexes (G4). Interaction of a selected porphyrin (Cu-TMPyPP) with oligonucleotide sequences either forming or not forming G4 was studied using cyclic voltammetry at hanging mercury drop electrode. The interaction is shown to be detectable by cyclic voltammetry as potential shift of a characteristic peak of Cu-TMPyPP which is observed for G4, single stranded as well as double stranded DNA. Nevertheless, further changes of this peak resulting from application of cyclic potential scans to the studied DNA before interaction with Cu-TMPyPP can be used to clearly distinguish G4 DNA, which can be applied as analytical tool for its detection.

Citovat

DOBROVODSKÝ, Daniel, Aleš DAŇHEL, Jana MALÍKOVÁ and Miroslav FOJTA. Electrochemical detection of G-quadruplex by interaction with porphyrin ligand. Online. In Nesmerak, K. PROCEEDINGS OF THE 15TH INTERNATIONAL STUDENTS CONFERENCE MODERN ANALYTICAL CHEMISTRY. PRAGUE: CHARLES UNIV, FAC SCIENCE, 2019, p. 215-219. ISBN 978-80-7444-068-7.

@inproceedings{1722203,
   author = {Dobrovodský, Daniel and Daňhel, Aleš and Malíková, Jana and Fojta, Miroslav},
   address = {PRAGUE},
   booktitle = {PROCEEDINGS OF THE 15TH INTERNATIONAL STUDENTS CONFERENCE MODERN ANALYTICAL CHEMISTRY},
   editor = {Nesmerak, K},
   keywords = {cyclic voltammetry; G-quadruplex; hanging mercury drop electrode; porphyrins},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {PRAGUE},
   isbn = {978-80-7444-068-7},
   pages = {215-219},
   publisher = {CHARLES UNIV, FAC SCIENCE},
   title = {Electrochemical detection of G-quadruplex by interaction with porphyrin ligand},
   year = {2019}
}

TY  - JOUR
ID  - 1722203
AU  - Dobrovodský, Daniel - Daňhel, Aleš - Malíková, Jana - Fojta, Miroslav
PY  - 2019
TI  - Electrochemical detection of G-quadruplex by interaction with porphyrin ligand
PB  - CHARLES UNIV, FAC SCIENCE
CY  - PRAGUE
SN  - 9788074440687
KW  - cyclic voltammetry
KW  - G-quadruplex
KW  - hanging mercury drop electrode
KW  - porphyrins
N2  - Porphyrins are well-known binders of DNA and can also specifically target guanine quadruplexes (G4). Interaction of a selected porphyrin (Cu-TMPyPP) with oligonucleotide sequences either forming or not forming G4 was studied using cyclic voltammetry at hanging mercury drop electrode. The interaction is shown to be detectable by cyclic voltammetry as potential shift of a characteristic peak of Cu-TMPyPP which is observed for G4, single stranded as well as double stranded DNA. Nevertheless, further changes of this peak resulting from application of cyclic potential scans to the studied DNA before interaction with Cu-TMPyPP can be used to clearly distinguish G4 DNA, which can be applied as analytical tool for its detection.
ER  -

DOBROVODSKÝ, Daniel, Aleš DAŇHEL, Jana MALÍKOVÁ and Miroslav FOJTA. Electrochemical detection of G-quadruplex by interaction with porphyrin ligand. Online. In Nesmerak, K. \textit{PROCEEDINGS OF THE 15TH INTERNATIONAL STUDENTS CONFERENCE MODERN ANALYTICAL CHEMISTRY}. PRAGUE: CHARLES UNIV, FAC SCIENCE, 2019, p.~215-219. ISBN~978-80-7444-068-7.

Detailed Information on Publication Record