Role of folding kinetics of secondary structures in telomeric
G-overhangs in the regulation of telomere maintenance
inSaccharomyces cerevisiae

J 2020

Role of folding kinetics of secondary structures in telomeric G-overhangs in the regulation of telomere maintenance inSaccharomyces cerevisiae

JURIKOVA, K.; Martin GAJARSKÝ; M. HAJIKAZEMI; J. NOSEK; K. PROCHAZKOVA et. al.

Základní údaje

Originální název

Role of folding kinetics of secondary structures in telomeric G-overhangs in the regulation of telomere maintenance inSaccharomyces cerevisiae

Autoři

JURIKOVA, K.; Martin GAJARSKÝ; M. HAJIKAZEMI; J. NOSEK; K. PROCHAZKOVA; K. PAESCHKE; Lukáš TRANTÍREK a L. TOMASKA

Vydání

Journal of Biological Chemistry, Bethesda, USA, Amer. Soc. Biochem. Mol. Biol. 2020, 0021-9258

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.157

Kód RIV

RIV/00216224:14740/20:00114648

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1074/jbc.RA120.012914

UT WoS

000550698000007

EID Scopus

2-s2.0-85087532995

Klíčová slova anglicky

telomere; telomerase; Saccharomyces cerevisiae; cell cycle; Cdc13; G-hairpin; G-quadruplex; folding kinetics

Štítky

CF BIC, CF NMR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 14. 10. 2024 15:51, Ing. Marie Švancarová

Anotace

V originále

The ends of eukaryotic chromosomes typically contain a 3? ssDNA G-rich protrusion (G-overhang). This overhang must be protected against detrimental activities of nucleases and of the DNA damage response machinery and participates in the regulation of telomerase, a ribonucleoprotein complex that maintains telomere integrity. These functions are mediated by DNA-binding proteins, such as Cdc13 inSaccharomyces cerevisiae, and the propensity of G-rich sequences to form various non-B DNA structures. Using CD and NMR spectroscopies, we show here that G-overhangs ofS. cerevisiaeform distinct Hoogsteen pairing?based secondary structures, depending on their length. Whereas short telomeric oligonucleotides form a G-hairpin, their longer counterparts form parallel and/or antiparallel G-quadruplexes (G4s). Regardless of their topologies, non-B DNA structures exhibited impaired binding to Cdc13in vitroas demonstrated by electrophoretic mobility shift assays. Importantly, whereas G4 structures formed relatively quickly, G-hairpins folded extremely slowly, indicating that short G-overhangs, which are typical for most of the cell cycle, are present predominantly as single-stranded oligonucleotides and are suitable substrates for Cdc13. Using ChIP, we show that the occurrence of G4 structures peaks at the late S phase, thus correlating with the accumulation of long G-overhangs. We present a model of how time- and length-dependent formation of non-B DNA structures at chromosomal termini participates in telomere maintenance.

Návaznosti

GA17-12075S, projekt VaV

Název: Polymorfní G-quadruplexy v promotorových oblastech genů

Investor: Grantová agentura ČR, Polymorfní G-quadruplexy v promotorových oblastech genů

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

NV19-08-00450, projekt VaV

Název: Atomárně rozlišená NMR spektroskopie in vivo jako nástroj pro biologické testování terapeuticky významných cílů v genomové ne-kanonické DNA a jejich interakcí s léčivy ve fenotypově diverzifikovaných nádorových buňkách.

Investor: Ministerstvo zdravotnictví ČR, Atomically resolved in vivo NMR spectroscopy as a novel tool for biological testing of therapeutically important genomic non-canonical DNA targets and their interactions with drugs in phenotypically diversified cancer cells.

90043, velká výzkumná infrastruktura

Název: CIISB

Citovat

JURIKOVA, K.; Martin GAJARSKÝ; M. HAJIKAZEMI; J. NOSEK; K. PROCHAZKOVA; K. PAESCHKE; Lukáš TRANTÍREK a L. TOMASKA. Role of folding kinetics of secondary structures in telomeric G-overhangs in the regulation of telomere maintenance inSaccharomyces cerevisiae. Journal of Biological Chemistry. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2020, roč. 295, č. 27, s. 8958-8971. ISSN 0021-9258. Dostupné z: https://doi.org/10.1074/jbc.RA120.012914.

@article{1724876,
   author = {Jurikova, K. and Gajarský, Martin and Hajikazemi, M. and Nosek, J. and Prochazkova, K. and Paeschke, K. and Trantírek, Lukáš and Tomaska, L.},
   article_location = {Bethesda, USA},
   article_number = {27},
   doi = {https://doi.org/10.1074/jbc.RA120.012914},
   keywords = {telomere; telomerase; Saccharomyces cerevisiae; cell cycle; Cdc13; G-hairpin; G-quadruplex; folding kinetics},
   language = {eng},
   issn = {0021-9258},
   journal = {Journal of Biological Chemistry},
   title = {Role of folding kinetics of secondary structures in telomeric G-overhangs in the regulation of telomere maintenance inSaccharomyces cerevisiae},
   url = {https://www.jbc.org/article/S0021-9258(17)50320-8/pdf},
   volume = {295},
   year = {2020}
}

TY  - JOUR
ID  - 1724876
AU  - Jurikova, K. - Gajarský, Martin - Hajikazemi, M. - Nosek, J. - Prochazkova, K. - Paeschke, K. - Trantírek, Lukáš - Tomaska, L.
PY  - 2020
TI  - Role of folding kinetics of secondary structures in telomeric G-overhangs in the regulation of telomere maintenance inSaccharomyces cerevisiae
JF  - Journal of Biological Chemistry
VL  - 295
IS  - 27
SP  - 8958-8971
EP  - 8958-8971
PB  - Amer. Soc. Biochem. Mol. Biol.
SN  - 00219258
KW  - telomere
KW  - telomerase
KW  - Saccharomyces cerevisiae
KW  - cell cycle
KW  - Cdc13
KW  - G-hairpin
KW  - G-quadruplex
KW  - folding kinetics
UR  - https://www.jbc.org/article/S0021-9258(17)50320-8/pdf
N2  - The ends of eukaryotic chromosomes typically contain a 3? ssDNA G-rich protrusion (G-overhang). This overhang must be protected against detrimental activities of nucleases and of the DNA damage response machinery and participates in the regulation of telomerase, a ribonucleoprotein complex that maintains telomere integrity. These functions are mediated by DNA-binding proteins, such as Cdc13 inSaccharomyces cerevisiae, and the propensity of G-rich sequences to form various non-B DNA structures. Using CD and NMR spectroscopies, we show here that G-overhangs ofS. cerevisiaeform distinct Hoogsteen pairing?based secondary structures, depending on their length. Whereas short telomeric oligonucleotides form a G-hairpin, their longer counterparts form parallel and/or antiparallel G-quadruplexes (G4s). Regardless of their topologies, non-B DNA structures exhibited impaired binding to Cdc13in vitroas demonstrated by electrophoretic mobility shift assays. Importantly, whereas G4 structures formed relatively quickly, G-hairpins folded extremely slowly, indicating that short G-overhangs, which are typical for most of the cell cycle, are present predominantly as single-stranded oligonucleotides and are suitable substrates for Cdc13. Using ChIP, we show that the occurrence of G4 structures peaks at the late S phase, thus correlating with the accumulation of long G-overhangs. We present a model of how time- and length-dependent formation of non-B DNA structures at chromosomal termini participates in telomere maintenance.
ER  -

JURIKOVA, K.; Martin GAJARSKÝ; M. HAJIKAZEMI; J. NOSEK; K. PROCHAZKOVA; K. PAESCHKE; Lukáš TRANTÍREK a L. TOMASKA. Role of folding kinetics of secondary structures in telomeric G-overhangs in the regulation of telomere maintenance inSaccharomyces cerevisiae. \textit{Journal of Biological Chemistry}. Bethesda, USA: Amer. Soc. Biochem. Mol. Biol., 2020, roč.~295, č.~27, s.~8958-8971. ISSN~0021-9258. Dostupné z: https://doi.org/10.1074/jbc.RA120.012914.

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