High fungal substrate specificity limits the utility of
environmental DNA to detect fungal diversity in bogs

J 2021

High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs

VAŠUTOVÁ, Martina, Martin JIROUŠEK and Michal HÁJEK

Basic information

Original name

High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs

Authors

VAŠUTOVÁ, Martina (203 Czech Republic), Martin JIROUŠEK (203 Czech Republic, guarantor, belonging to the institution) and Michal HÁJEK (203 Czech Republic, belonging to the institution)

Edition

Ecological Indicators, Amsterdam, Elsevier, 2021, 1470-160X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10511 Environmental sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.263

RIV identification code

RIV/00216224:14310/21:00118817

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.ecolind.2020.107009

UT WoS

000604882200013

Keywords in English

Biodiversity; eDNA; Fruitbody; Fungi; Peatland

Abstract

V originále

Fungi are a highly diverse group of organisms and play a significant role in decomposition and carbon cycling in boreal ecosystems. To determine how fungal communities are structured in peat bogs and how to obtain representative samples for monitoring fungal community changes, we separately sampled and sequenced (ITS2, Illumina MiSeq) peat, mixed litter and litter from individual dominant plant species in five permanent plots in raised bogs in the Jizerské Hory Mountains (Czech Republic). In total, we detected 68–103 OTUs per plot. The fungal assemblages were mostly influenced by substrate identity, whereas the effect of the site was minimal. Only a few identified OTUs behave like generalists. The most specific fungi were found in dead parts of Calluna vulgaris, Trichophorum cespitosum and Drosera rotundifolia. The high substrate heterogeneity means that sequencing soil core samples does not provide an exhaustive inventory of the fungal diversity in bogs. Poor correspondence was observed between the recorded OTUs and either visually inspected fruitbodies or literature reports on the fungal taxa associated with the analysed substrates.

Links

GX19-28491X, research and development project

Name: Centrum pro evropské vegetační syntézy (CEVS) (Acronym: CEVS)

Investor: Czech Science Foundation

Citovat

VAŠUTOVÁ, Martina, Martin JIROUŠEK and Michal HÁJEK. High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs. Ecological Indicators. Amsterdam: Elsevier, 2021, vol. 121, February, p. 1-10. ISSN 1470-160X. Available from: https://dx.doi.org/10.1016/j.ecolind.2020.107009.

@article{1725979,
   author = {Vašutová, Martina and Jiroušek, Martin and Hájek, Michal},
   article_location = {Amsterdam},
   article_number = {February},
   doi = {http://dx.doi.org/10.1016/j.ecolind.2020.107009},
   keywords = {Biodiversity; eDNA; Fruitbody; Fungi; Peatland},
   language = {eng},
   issn = {1470-160X},
   journal = {Ecological Indicators},
   title = {High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs},
   url = {https://doi.org/10.1016/j.ecolind.2020.107009},
   volume = {121},
   year = {2021}
}

TY  - JOUR
ID  - 1725979
AU  - Vašutová, Martina - Jiroušek, Martin - Hájek, Michal
PY  - 2021
TI  - High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs
JF  - Ecological Indicators
VL  - 121
IS  - February
SP  - 1-10
EP  - 1-10
PB  - Elsevier
SN  - 1470160X
KW  - Biodiversity
KW  - eDNA
KW  - Fruitbody
KW  - Fungi
KW  - Peatland
UR  - https://doi.org/10.1016/j.ecolind.2020.107009
L2  - https://doi.org/10.1016/j.ecolind.2020.107009
N2  - Fungi are a highly diverse group of organisms and play a significant role in decomposition and carbon cycling in boreal ecosystems. To determine how fungal communities are structured in peat bogs and how to obtain representative samples for monitoring fungal community changes, we separately sampled and sequenced (ITS2, Illumina MiSeq) peat, mixed litter and litter from individual dominant plant species in five permanent plots in raised bogs in the Jizerské Hory Mountains (Czech Republic). In total, we detected 68–103 OTUs per plot. The fungal assemblages were mostly influenced by substrate identity, whereas the effect of the site was minimal. Only a few identified OTUs behave like generalists. The most specific fungi were found in dead parts of Calluna vulgaris, Trichophorum cespitosum and Drosera rotundifolia. The high substrate heterogeneity means that sequencing soil core samples does not provide an exhaustive inventory of the fungal diversity in bogs. Poor correspondence was observed between the recorded OTUs and either visually inspected fruitbodies or literature reports on the fungal taxa associated with the analysed substrates.
ER  -

VAŠUTOVÁ, Martina, Martin JIROUŠEK and Michal HÁJEK. High fungal substrate specificity limits the utility of environmental DNA to detect fungal diversity in bogs. \textit{Ecological Indicators}. Amsterdam: Elsevier, 2021, vol.~121, February, p.~1-10. ISSN~1470-160X. Available from: https://dx.doi.org/10.1016/j.ecolind.2020.107009.

Detailed Information on Publication Record