| BROFT, P., Šimon DŽATKO, Michaela KRAFČÍKOVÁ, A. WACKER, Robert HANSEL-HERTSCH, Volker DOTSCH, Lukáš TRANTÍREK and Harald SCHWALBE. In-Cell NMR Spectroscopy of Functional Riboswitch Aptamers in Eukaryotic Cells. Angewandte Chemie International Edition. Weinheim: Wiley-VCH Verlag, 2021, vol. 60, No 2, p. 865-872, 10 pp. ISSN 1433-7851. Available from: https://dx.doi.org/10.1002/anie.202007184. |
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@article{1726480,
author = {Broft, P. and Džatko, Šimon and Krafčíková, Michaela and Wacker, A. and HanselandHertsch, Robert and Dotsch, Volker and Trantírek, Lukáš and Schwalbe, Harald},
article_location = {Weinheim},
article_number = {2},
doi = {http://dx.doi.org/10.1002/anie.202007184},
keywords = {aptamers; 2' -deoxyguanosine riboswitch; HeLa cells; RNA structures; structural biology},
language = {eng},
issn = {1433-7851},
journal = {Angewandte Chemie International Edition},
title = {In-Cell NMR Spectroscopy of Functional Riboswitch Aptamers in Eukaryotic Cells},
url = {https://doi.org/10.1002/anie.202007184},
volume = {60},
year = {2021}
}
TY - JOUR
ID - 1726480
AU - Broft, P. - Džatko, Šimon - Krafčíková, Michaela - Wacker, A. - Hansel-Hertsch, Robert - Dotsch, Volker - Trantírek, Lukáš - Schwalbe, Harald
PY - 2021
TI - In-Cell NMR Spectroscopy of Functional Riboswitch Aptamers in Eukaryotic Cells
JF - Angewandte Chemie International Edition
VL - 60
IS - 2
SP - 865-872
EP - 865-872
PB - Wiley-VCH Verlag
SN - 14337851
KW - aptamers
KW - 2' -deoxyguanosine riboswitch
KW - HeLa cells
KW - RNA structures
KW - structural biology
UR - https://doi.org/10.1002/anie.202007184
L2 - https://doi.org/10.1002/anie.202007184
N2 - We report here the in-cell NMR-spectroscopic observation of the binding of the cognate ligand 2 '-deoxyguanosine to the aptamer domain of the bacterial 2 '-deoxyguanosine-sensing riboswitch in eukaryotic cells, namely Xenopus laevis oocytes and in human HeLa cells. The riboswitch is sufficiently stable in both cell types to allow for detection of binding of the ligand to the riboswitch. Most importantly, we show that the binding mode established by in vitro characterization of this prokaryotic riboswitch is maintained in eukaryotic cellular environment. Our data also bring important methodological insights: Thus far, in-cell NMR studies on RNA in mammalian cells have been limited to investigations of short (<15 nt) RNA fragments that were extensively modified by protecting groups to limit their degradation in the intracellular space. Here, we show that the in-cell NMR setup can be adjusted for characterization of much larger (approximate to 70 nt) functional and chemically non-modified RNA.
ER -
BROFT, P., Šimon DŽATKO, Michaela KRAFČÍKOVÁ, A. WACKER, Robert HANSEL-HERTSCH, Volker DOTSCH, Lukáš TRANTÍREK and Harald SCHWALBE. In-Cell NMR Spectroscopy of Functional Riboswitch Aptamers in Eukaryotic Cells. \textit{Angewandte Chemie International Edition}. Weinheim: Wiley-VCH Verlag, 2021, vol.~60, No~2, p.~865-872, 10 pp. ISSN~1433-7851. Available from: https://dx.doi.org/10.1002/anie.202007184.
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