Detailed Information on Publication Record
2021
In-Cell NMR Spectroscopy of Functional Riboswitch Aptamers in Eukaryotic Cells
BROFT, P., Šimon DŽATKO, Michaela KRAFČÍKOVÁ, A. WACKER, Robert HANSEL-HERTSCH et. al.Basic information
Original name
In-Cell NMR Spectroscopy of Functional Riboswitch Aptamers in Eukaryotic Cells
Authors
BROFT, P., Šimon DŽATKO (703 Slovakia, belonging to the institution), Michaela KRAFČÍKOVÁ (703 Slovakia, belonging to the institution), A. WACKER, Robert HANSEL-HERTSCH, Volker DOTSCH, Lukáš TRANTÍREK (203 Czech Republic, guarantor, belonging to the institution) and Harald SCHWALBE
Edition
Angewandte Chemie International Edition, Weinheim, Wiley-VCH Verlag, 2021, 1433-7851
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10608 Biochemistry and molecular biology
Country of publisher
Germany
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 16.823
RIV identification code
RIV/00216224:14740/21:00118818
Organization unit
Central European Institute of Technology
UT WoS
000591274200001
Keywords in English
aptamers; 2' -deoxyguanosine riboswitch; HeLa cells; RNA structures; structural biology
Tags
International impact, Reviewed
Změněno: 4/3/2022 13:34, Mgr. Pavla Foltynová, Ph.D.
Abstract
V originále
We report here the in-cell NMR-spectroscopic observation of the binding of the cognate ligand 2 '-deoxyguanosine to the aptamer domain of the bacterial 2 '-deoxyguanosine-sensing riboswitch in eukaryotic cells, namely Xenopus laevis oocytes and in human HeLa cells. The riboswitch is sufficiently stable in both cell types to allow for detection of binding of the ligand to the riboswitch. Most importantly, we show that the binding mode established by in vitro characterization of this prokaryotic riboswitch is maintained in eukaryotic cellular environment. Our data also bring important methodological insights: Thus far, in-cell NMR studies on RNA in mammalian cells have been limited to investigations of short (<15 nt) RNA fragments that were extensively modified by protecting groups to limit their degradation in the intracellular space. Here, we show that the in-cell NMR setup can be adjusted for characterization of much larger (approximate to 70 nt) functional and chemically non-modified RNA.
Links
| GX19-26041X, research and development project |
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| LM2015043, research and development project |
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| LM2015062, research and development project |
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| LM2015064, research and development project |
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| LQ1601, research and development project |
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| 871037, interní kód MU |
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