| DOCEKALOVA, M., D. UHLIROVA, M. STANKOVA, M. KEPINSKA, J. SOCHOR, H. MILNEROWICZ, Petr BABULA, C. FERNANDEZ, M. BRAZDOVA, J. ZIDKOVA, P. SUCHY and R. KIZEK. CHARACTERISATION OF PEROXIDASE-LIKE ACTIVITY OF THERMALLY SYNTHESIZED GOLD NANOPARTICLES. SLEZSKA: TANGER LTD, 2017, p. 429-434. |
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@article{1726659,
author = {Docekalova, M. and Uhlirova, D. and Stankova, M. and Kepinska, M. and Sochor, J. and Milnerowicz, H. and Babula, Petr and Fernandez, C. and Brazdova, M. and Zidkova, J. and Suchy, P. and Kizek, R.},
article_location = {SLEZSKA},
keywords = {Gold nanoparticles; electrochemistry; spectrophotometry; enzymatic reaction; ELISA; nanomedicine},
language = {eng},
title = {CHARACTERISATION OF PEROXIDASE-LIKE ACTIVITY OF THERMALLY SYNTHESIZED GOLD NANOPARTICLES},
year = {2017}
}
TY - JOUR
ID - 1726659
AU - Docekalova, M. - Uhlirova, D. - Stankova, M. - Kepinska, M. - Sochor, J. - Milnerowicz, H. - Babula, Petr - Fernandez, C. - Brazdova, M. - Zidkova, J. - Suchy, P. - Kizek, R.
PY - 2017
TI - CHARACTERISATION OF PEROXIDASE-LIKE ACTIVITY OF THERMALLY SYNTHESIZED GOLD NANOPARTICLES
SP - 429-434
EP - 429-434
PB - TANGER LTD
KW - Gold nanoparticles
KW - electrochemistry
KW - spectrophotometry
KW - enzymatic reaction
KW - ELISA
KW - nanomedicine
N2 - The development of nanotechnologies has attracted a lot of interest since it brings new possibilities in the field of nanomedical applications, from diagnostic methods to targeted nanotransporters. Gold nanoparticles (AuNPs) belong to a group of nanomaterials with significant peroxidase-like activity. We have successfully synthesized AuNPs thermally over a range of temperatures from 20 to 100 degrees C. The AuNPs size varied from 17 to 37 nm and the zeta potential was found to vary from -32 to -44 mV, in which the maximum absorption maximum was detected at 529 nm. Peroxidase-like activity was monitored in the presence of TMB (1 mM) and hydrogen peroxide (10 mM). The proposed system based on peroxidase-like activity was further evaluated in immune detection of human IgG, in which anti-IgG antibody was modified with AuNPs.
ER -
DOCEKALOVA, M., D. UHLIROVA, M. STANKOVA, M. KEPINSKA, J. SOCHOR, H. MILNEROWICZ, Petr BABULA, C. FERNANDEZ, M. BRAZDOVA, J. ZIDKOVA, P. SUCHY and R. KIZEK. CHARACTERISATION OF PEROXIDASE-LIKE ACTIVITY OF THERMALLY SYNTHESIZED GOLD NANOPARTICLES. SLEZSKA: TANGER LTD, 2017, p.~429-434.
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