Další formáty:
BibTeX
LaTeX
RIS
@article{1727095,
author = {Hassan, STS and BerchovaandBimova, K and Sudomova, M and Malaník, Milan and Šmejkal, Karel and Rengasamy, KRR},
article_location = {BASEL},
article_number = {9},
doi = {http://dx.doi.org/10.3390/jcm7090283},
keywords = {anticancer; antihypertensive; HSV-2; Thymus bovei Benth; essential oil; phytochemical profile},
language = {eng},
issn = {2077-0383},
journal = {Journal of Clinical Medicine},
title = {In Vitro Study of Multi-Therapeutic Properties of Thymus bovei Benth. Essential Oil and Its Main Component for Promoting Their Use in Clinical Practice},
volume = {7},
year = {2018}
}
TY - JOUR
ID - 1727095
AU - Hassan, STS - Berchova-Bimova, K - Sudomova, M - Malaník, Milan - Šmejkal, Karel - Rengasamy, KRR
PY - 2018
TI - In Vitro Study of Multi-Therapeutic Properties of Thymus bovei Benth. Essential Oil and Its Main Component for Promoting Their Use in Clinical Practice
JF - Journal of Clinical Medicine
VL - 7
IS - 9
PB - MDPI
SN - 20770383
KW - anticancer
KW - antihypertensive
KW - HSV-2
KW - Thymus bovei Benth
KW - essential oil
KW - phytochemical profile
N2 - Thymus bovei Benth. (TB) is an important plant in the traditional medicine of the Mediterranean region. This study investigates the health-promoting properties of TB essential oil (TB-EO) for its possible use in clinical practice with regards to its cytotoxic, anti-herpes simplex virus type 2 (HSV-2), and antihypertensive (through inhibition of human angiotensin-converting enzyme; ACE) properties. The phytochemical profile of EO (99.9%) was analyzed by Gas Chromatography with Flame-Ionization Detection (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS). In this study, all biological methods were performed at the level of in vitro studies. The results showed that TB-EO exerted remarked cytotoxic properties against human cervical carcinoma cells, colon cancer cells, and lung adenocarcinoma cells with the half-maximal inhibitory concentration (IC50) values of 7.22, 9.30, and 8.62 mu g/mL, respectively, in comparison with that of standard anticancer drug cisplatin with IC50 values of 4.24, 5.21, and 5.43 mu g/mL, respectively. Fascinatingly, TB-EO showed very weak cytotoxicity on the healthy human fetal lung fibroblast cells with an IC50 value of 118.34 mu g/mL compared with that of cisplatin (IC50 = 10.08 mu g/mL). TB-EO, its main component geraniol, TB-EO combined with acyclovir (ACV) along with standard ACV, have displayed pronounced inhibitory properties against the replication of HSV-2 with the half-maximal effective concentration (EC50) values of 2.13, 1.92, 0.81 and 1.94 mu g/mL, respectively, with corresponding selectivity indices (SI) 98.59, 109.38, 259.26 and 108.25, respectively. TB-EO and geraniol at a concentration of 15 mu g/mL showed prominent inhibitory activities against ACE with % of inhibition 95.4% and 92.2%, respectively, compared with that of standard inhibitor captopril (99.8%; 15 mu g/mL). Molecular docking studies were performed to unveil the mechanism of action of geraniol as well as structural parameters necessary for anti-HSV-2 activity (through the inhibition of HSV-2 protease) and ACE inhibition. This is the first report on the chemical composition of Egyptian TB-EO along with the above-mentioned biological activities. Our results may be considered as novel findings in the course of a search for new and active anticancer, anti-HSV-2 and antihypertensive agents, and expand the medicinal value of this plant and its phytochemicals in clinical practice.
ER -
HASSAN, STS, K BERCHOVA-BIMOVA, M SUDOMOVA, Milan MALANÍK, Karel ŠMEJKAL a KRR RENGASAMY. In Vitro Study of Multi-Therapeutic Properties of Thymus bovei Benth. Essential Oil and Its Main Component for Promoting Their Use in Clinical Practice. Online. \textit{Journal of Clinical Medicine}. BASEL: MDPI, 2018, roč.~7, č.~9, 15 s. ISSN~2077-0383. Dostupné z: https://dx.doi.org/10.3390/jcm7090283. [citováno 2024-04-23]
|
