In Vitro Study of Multi-Therapeutic Properties of Thymus bovei
Benth. Essential Oil and Its Main Component for Promoting Their
Use in Clinical Practice

J 2018

In Vitro Study of Multi-Therapeutic Properties of Thymus bovei Benth. Essential Oil and Its Main Component for Promoting Their Use in Clinical Practice

HASSAN, STS, K BERCHOVA-BIMOVA, M SUDOMOVA, Milan MALANÍK, Karel ŠMEJKAL et. al.

Basic information

Original name

In Vitro Study of Multi-Therapeutic Properties of Thymus bovei Benth. Essential Oil and Its Main Component for Promoting Their Use in Clinical Practice

Authors

HASSAN, STS, K BERCHOVA-BIMOVA, M SUDOMOVA, Milan MALANÍK, Karel ŠMEJKAL and KRR RENGASAMY

Edition

Journal of Clinical Medicine, BASEL, MDPI, 2018, 2077-0383

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 5.688

Organization unit

Faculty of Pharmacy

DOI

http://dx.doi.org/10.3390/jcm7090283

UT WoS

000445635800057

Keywords in English

anticancer; antihypertensive; HSV-2; Thymus bovei Benth; essential oil; phytochemical profile

Abstract

V originále

Thymus bovei Benth. (TB) is an important plant in the traditional medicine of the Mediterranean region. This study investigates the health-promoting properties of TB essential oil (TB-EO) for its possible use in clinical practice with regards to its cytotoxic, anti-herpes simplex virus type 2 (HSV-2), and antihypertensive (through inhibition of human angiotensin-converting enzyme; ACE) properties. The phytochemical profile of EO (99.9%) was analyzed by Gas Chromatography with Flame-Ionization Detection (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS). In this study, all biological methods were performed at the level of in vitro studies. The results showed that TB-EO exerted remarked cytotoxic properties against human cervical carcinoma cells, colon cancer cells, and lung adenocarcinoma cells with the half-maximal inhibitory concentration (IC50) values of 7.22, 9.30, and 8.62 mu g/mL, respectively, in comparison with that of standard anticancer drug cisplatin with IC50 values of 4.24, 5.21, and 5.43 mu g/mL, respectively. Fascinatingly, TB-EO showed very weak cytotoxicity on the healthy human fetal lung fibroblast cells with an IC50 value of 118.34 mu g/mL compared with that of cisplatin (IC50 = 10.08 mu g/mL). TB-EO, its main component geraniol, TB-EO combined with acyclovir (ACV) along with standard ACV, have displayed pronounced inhibitory properties against the replication of HSV-2 with the half-maximal effective concentration (EC50) values of 2.13, 1.92, 0.81 and 1.94 mu g/mL, respectively, with corresponding selectivity indices (SI) 98.59, 109.38, 259.26 and 108.25, respectively. TB-EO and geraniol at a concentration of 15 mu g/mL showed prominent inhibitory activities against ACE with % of inhibition 95.4% and 92.2%, respectively, compared with that of standard inhibitor captopril (99.8%; 15 mu g/mL). Molecular docking studies were performed to unveil the mechanism of action of geraniol as well as structural parameters necessary for anti-HSV-2 activity (through the inhibition of HSV-2 protease) and ACE inhibition. This is the first report on the chemical composition of Egyptian TB-EO along with the above-mentioned biological activities. Our results may be considered as novel findings in the course of a search for new and active anticancer, anti-HSV-2 and antihypertensive agents, and expand the medicinal value of this plant and its phytochemicals in clinical practice.

Citovat

HASSAN, STS, K BERCHOVA-BIMOVA, M SUDOMOVA, Milan MALANÍK, Karel ŠMEJKAL and KRR RENGASAMY. In Vitro Study of Multi-Therapeutic Properties of Thymus bovei Benth. Essential Oil and Its Main Component for Promoting Their Use in Clinical Practice. Journal of Clinical Medicine. BASEL: MDPI, 2018, vol. 7, No 9, 15 pp. ISSN 2077-0383. Available from: https://dx.doi.org/10.3390/jcm7090283.

@article{1727095,
   author = {Hassan, STS and BerchovaandBimova, K and Sudomova, M and Malaník, Milan and Šmejkal, Karel and Rengasamy, KRR},
   article_location = {BASEL},
   article_number = {9},
   doi = {http://dx.doi.org/10.3390/jcm7090283},
   keywords = {anticancer; antihypertensive; HSV-2; Thymus bovei Benth; essential oil; phytochemical profile},
   language = {eng},
   issn = {2077-0383},
   journal = {Journal of Clinical Medicine},
   title = {In Vitro Study of Multi-Therapeutic Properties of Thymus bovei Benth. Essential Oil and Its Main Component for Promoting Their Use in Clinical Practice},
   volume = {7},
   year = {2018}
}

TY  - JOUR
ID  - 1727095
AU  - Hassan, STS - Berchova-Bimova, K - Sudomova, M - Malaník, Milan - Šmejkal, Karel - Rengasamy, KRR
PY  - 2018
TI  - In Vitro Study of Multi-Therapeutic Properties of Thymus bovei Benth. Essential Oil and Its Main Component for Promoting Their Use in Clinical Practice
JF  - Journal of Clinical Medicine
VL  - 7
IS  - 9
PB  - MDPI
SN  - 20770383
KW  - anticancer
KW  - antihypertensive
KW  - HSV-2
KW  - Thymus bovei Benth
KW  - essential oil
KW  - phytochemical profile
N2  - Thymus bovei Benth. (TB) is an important plant in the traditional medicine of the Mediterranean region. This study investigates the health-promoting properties of TB essential oil (TB-EO) for its possible use in clinical practice with regards to its cytotoxic, anti-herpes simplex virus type 2 (HSV-2), and antihypertensive (through inhibition of human angiotensin-converting enzyme; ACE) properties. The phytochemical profile of EO (99.9%) was analyzed by Gas Chromatography with Flame-Ionization Detection (GC-FID) and Gas Chromatography-Mass Spectrometry (GC-MS). In this study, all biological methods were performed at the level of in vitro studies. The results showed that TB-EO exerted remarked cytotoxic properties against human cervical carcinoma cells, colon cancer cells, and lung adenocarcinoma cells with the half-maximal inhibitory concentration (IC50) values of 7.22, 9.30, and 8.62 mu g/mL, respectively, in comparison with that of standard anticancer drug cisplatin with IC50 values of 4.24, 5.21, and 5.43 mu g/mL, respectively. Fascinatingly, TB-EO showed very weak cytotoxicity on the healthy human fetal lung fibroblast cells with an IC50 value of 118.34 mu g/mL compared with that of cisplatin (IC50 = 10.08 mu g/mL). TB-EO, its main component geraniol, TB-EO combined with acyclovir (ACV) along with standard ACV, have displayed pronounced inhibitory properties against the replication of HSV-2 with the half-maximal effective concentration (EC50) values of 2.13, 1.92, 0.81 and 1.94 mu g/mL, respectively, with corresponding selectivity indices (SI) 98.59, 109.38, 259.26 and 108.25, respectively. TB-EO and geraniol at a concentration of 15 mu g/mL showed prominent inhibitory activities against ACE with % of inhibition 95.4% and 92.2%, respectively, compared with that of standard inhibitor captopril (99.8%; 15 mu g/mL). Molecular docking studies were performed to unveil the mechanism of action of geraniol as well as structural parameters necessary for anti-HSV-2 activity (through the inhibition of HSV-2 protease) and ACE inhibition. This is the first report on the chemical composition of Egyptian TB-EO along with the above-mentioned biological activities. Our results may be considered as novel findings in the course of a search for new and active anticancer, anti-HSV-2 and antihypertensive agents, and expand the medicinal value of this plant and its phytochemicals in clinical practice.
ER  -

HASSAN, STS, K BERCHOVA-BIMOVA, M SUDOMOVA, Milan MALANÍK, Karel ŠMEJKAL and KRR RENGASAMY. In Vitro Study of Multi-Therapeutic Properties of Thymus bovei Benth. Essential Oil and Its Main Component for Promoting Their Use in Clinical Practice. \textit{Journal of Clinical Medicine}. BASEL: MDPI, 2018, vol.~7, No~9, 15 pp. ISSN~2077-0383. Available from: https://dx.doi.org/10.3390/jcm7090283.

Detailed Information on Publication Record