Unique coated neusilin pellets with a more distinct and fast
visual detection of nerve agents and other cholinesterase
inhibitors

J 2020

Unique coated neusilin pellets with a more distinct and fast visual detection of nerve agents and other cholinesterase inhibitors

ZEMAN, Jiří, David VETCHÝ, Sylvie PAVLOKOVÁ, Aleš FRANC, Vladimír PITSCHMANN et. al.

Basic information

Original name

Unique coated neusilin pellets with a more distinct and fast visual detection of nerve agents and other cholinesterase inhibitors

Authors

ZEMAN, Jiří (203 Czech Republic, belonging to the institution), David VETCHÝ (203 Czech Republic, guarantor, belonging to the institution), Sylvie PAVLOKOVÁ (203 Czech Republic, belonging to the institution), Aleš FRANC (203 Czech Republic, belonging to the institution) and Vladimír PITSCHMANN (203 Czech Republic)

Edition

Journal of Pharmaceutical and Biomedical Analysis, Elsevier, 2020, 0731-7085

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.935

RIV identification code

RIV/00216224:14160/20:00118674

Organization unit

Faculty of Pharmacy

DOI

http://dx.doi.org/10.1016/j.jpba.2019.113004

UT WoS

000508752400057

Keywords in English

Detection tube; Nerve agent; Neusilin; Cholinesterase; Color transition; Stability

Abstract

V originále

Pellets with an immobilized enzyme (acetyl- or butyrylcholinesterase) are the up-to-date type of carriers used for the detection of nerve agents (soman, sarin, tabun, VX, Novichok) and other cholinesterase inhibitors such as organophosphate and carbamate insecticides (parathion, malathion). They are used in the glass detection tubes as a layer containing the enzyme together with the second layer, which contains a colorimetric reagent and substrate. The detection method is based on the visually or spectrophotometrically observable Ellman's reaction, which develops a yellow color in the absence of the cholinesterase inhibitor; otherwise, the detector preserves its original color (preferably white). This reaction occurs very fast and has a high sensitivity to nerve agents but it suffers from an indistinctive color transition from white to yellow. In the presented study, a new approach with the use of the synergic effect of magnesium aluminometasilicate with a high surface area marketed as Neusilin®US2 and a protective semipermeable Eudragit® RL layer was utilized. The prepared pellets have been evaluated for their properties such as the activity of the enzyme, intensity of the developed yellow color, sensitivity to cholinesterase inhibitor physostigmine, which acts as a nerve agent simulant, and physical parameters such as hardness, pycnometric density and sphericity. After the initial evaluation, all samples underwent a stability test under three different storage conditions for 24 months during which they were evaluated at given time points (0, 3, 6, 12 and 24 months). It was found that the prepared samples achieved a much higher intensity of developed yellow color than in the published studies while maintaining similar or better sensitivity, speed of detection and suitable physico-chemical properties.

Links

VI20192022172, research and development project

Name: Detekční trubičky druhé generace pro kontinuální a opakované monitorování nervově paralytických a jiných toxických látek

Investor: Ministry of the Interior of the CR

Citovat

ZEMAN, Jiří, David VETCHÝ, Sylvie PAVLOKOVÁ, Aleš FRANC and Vladimír PITSCHMANN. Unique coated neusilin pellets with a more distinct and fast visual detection of nerve agents and other cholinesterase inhibitors. Journal of Pharmaceutical and Biomedical Analysis. Elsevier, 2020, vol. 179, February, p. 1-9. ISSN 0731-7085. Available from: https://dx.doi.org/10.1016/j.jpba.2019.113004.

@article{1728019,
   author = {Zeman, Jiří and Vetchý, David and Pavloková, Sylvie and Franc, Aleš and Pitschmann, Vladimír},
   article_number = {February},
   doi = {http://dx.doi.org/10.1016/j.jpba.2019.113004},
   keywords = {Detection tube; Nerve agent; Neusilin; Cholinesterase; Color transition; Stability},
   language = {eng},
   issn = {0731-7085},
   journal = {Journal of Pharmaceutical and Biomedical Analysis},
   title = {Unique coated neusilin pellets with a more distinct and fast visual detection of nerve agents and other cholinesterase inhibitors},
   url = {http://www.sciencedirect.com/science/article/abs/pii/S0731708519316590?via%3Dihub},
   volume = {179},
   year = {2020}
}

TY  - JOUR
ID  - 1728019
AU  - Zeman, Jiří - Vetchý, David - Pavloková, Sylvie - Franc, Aleš - Pitschmann, Vladimír
PY  - 2020
TI  - Unique coated neusilin pellets with a more distinct and fast visual detection of nerve agents and other cholinesterase inhibitors
JF  - Journal of Pharmaceutical and Biomedical Analysis
VL  - 179
IS  - February
SP  - 1-9
EP  - 1-9
PB  - Elsevier
SN  - 07317085
KW  - Detection tube
KW  - Nerve agent
KW  - Neusilin
KW  - Cholinesterase
KW  - Color transition
KW  - Stability
UR  - http://www.sciencedirect.com/science/article/abs/pii/S0731708519316590?via%3Dihub
N2  - Pellets with an immobilized enzyme (acetyl- or butyrylcholinesterase) are the up-to-date type of carriers used for the detection of nerve agents (soman, sarin, tabun, VX, Novichok) and other cholinesterase inhibitors such as organophosphate and carbamate insecticides (parathion, malathion). They are used in the glass detection tubes as a layer containing the enzyme together with the second layer, which contains a colorimetric reagent and substrate. The detection method is based on the visually or spectrophotometrically observable Ellman's reaction, which develops a yellow color in the absence of the cholinesterase inhibitor; otherwise, the detector preserves its original color (preferably white). This reaction occurs very fast and has a high sensitivity to nerve agents but it suffers from an indistinctive color transition from white to yellow. In the presented study, a new approach with the use of the synergic effect of magnesium aluminometasilicate with a high surface area marketed as Neusilin®US2 and a protective semipermeable Eudragit® RL layer was utilized. The prepared pellets have been evaluated for their properties such as the activity of the enzyme, intensity of the developed yellow color, sensitivity to cholinesterase inhibitor physostigmine, which acts as a nerve agent simulant, and physical parameters such as hardness, pycnometric density and sphericity. After the initial evaluation, all samples underwent a stability test under three different storage conditions for 24 months during which they were evaluated at given time points (0, 3, 6, 12 and 24 months). It was found that the prepared samples achieved a much higher intensity of developed yellow color than in the published studies while maintaining similar or better sensitivity, speed of detection and suitable physico-chemical properties.
ER  -

ZEMAN, Jiří, David VETCHÝ, Sylvie PAVLOKOVÁ, Aleš FRANC and Vladimír PITSCHMANN. Unique coated neusilin pellets with a more distinct and fast visual detection of nerve agents and other cholinesterase inhibitors. \textit{Journal of Pharmaceutical and Biomedical Analysis}. Elsevier, 2020, vol.~179, February, p.~1-9. ISSN~0731-7085. Available from: https://dx.doi.org/10.1016/j.jpba.2019.113004.

Detailed Information on Publication Record