ZEMAN, Jiří, David VETCHÝ, Sylvie PAVLOKOVÁ, Aleš FRANC a Vladimír PITSCHMANN. Unique coated neusilin pellets with a more distinct and fast visual detection of nerve agents and other cholinesterase inhibitors. Journal of Pharmaceutical and Biomedical Analysis. Elsevier, 2020, roč. 179, February, s. 1-9. ISSN 0731-7085. doi:10.1016/j.jpba.2019.113004.
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Základní údaje
Originální název Unique coated neusilin pellets with a more distinct and fast visual detection of nerve agents and other cholinesterase inhibitors
Autoři ZEMAN, Jiří (203 Česká republika, domácí), David VETCHÝ (203 Česká republika, garant, domácí), Sylvie PAVLOKOVÁ (203 Česká republika, domácí), Aleš FRANC (203 Česká republika, domácí) a Vladimír PITSCHMANN (203 Česká republika).
Vydání Journal of Pharmaceutical and Biomedical Analysis, Elsevier, 2020, 0731-7085.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 30104 Pharmacology and pharmacy
Stát vydavatele Nizozemsko
Utajení není předmětem státního či obchodního tajemství
WWW URL
Impakt faktor Impact factor: 3.935
Kód RIV RIV/00216224:14160/20:00118674
Organizační jednotka Farmaceutická fakulta
Doi http://dx.doi.org/10.1016/j.jpba.2019.113004
UT WoS 000508752400057
Klíčová slova anglicky Detection tube; Nerve agent; Neusilin; Cholinesterase; Color transition; Stability
Štítky rivok, ÚFT
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Mgr. Hana Hurtová, učo 244985. Změněno: 18. 6. 2021 09:07.
Anotace
Pellets with an immobilized enzyme (acetyl- or butyrylcholinesterase) are the up-to-date type of carriers used for the detection of nerve agents (soman, sarin, tabun, VX, Novichok) and other cholinesterase inhibitors such as organophosphate and carbamate insecticides (parathion, malathion). They are used in the glass detection tubes as a layer containing the enzyme together with the second layer, which contains a colorimetric reagent and substrate. The detection method is based on the visually or spectrophotometrically observable Ellman's reaction, which develops a yellow color in the absence of the cholinesterase inhibitor; otherwise, the detector preserves its original color (preferably white). This reaction occurs very fast and has a high sensitivity to nerve agents but it suffers from an indistinctive color transition from white to yellow. In the presented study, a new approach with the use of the synergic effect of magnesium aluminometasilicate with a high surface area marketed as Neusilin®US2 and a protective semipermeable Eudragit® RL layer was utilized. The prepared pellets have been evaluated for their properties such as the activity of the enzyme, intensity of the developed yellow color, sensitivity to cholinesterase inhibitor physostigmine, which acts as a nerve agent simulant, and physical parameters such as hardness, pycnometric density and sphericity. After the initial evaluation, all samples underwent a stability test under three different storage conditions for 24 months during which they were evaluated at given time points (0, 3, 6, 12 and 24 months). It was found that the prepared samples achieved a much higher intensity of developed yellow color than in the published studies while maintaining similar or better sensitivity, speed of detection and suitable physico-chemical properties.
Návaznosti
VI20192022172, projekt VaVNázev: Detekční trubičky druhé generace pro kontinuální a opakované monitorování nervově paralytických a jiných toxických látek
Investor: Ministerstvo vnitra ČR, Detekční trubičky druhé generace pro kontinuální a opakované monitorování nervově paralytických a jiných toxických látek
VytisknoutZobrazeno: 6. 2. 2023 21:17