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@article{1728040,
author = {Zeman, Jiří and Pavloková, Sylvie and Vetchý, David and Pitschmann, Vladimír},
article_number = {1},
keywords = {ethylcellulose; pellets; cholinesterase; detection tube; nerve agent},
language = {eng},
issn = {1210-7816},
journal = {Česká a slovenská farmacie},
title = {Double-coated pellets with semipermeable ethylcellulose coating for detection of cholinesterase inhibitors},
url = {http://www.prolekare.cz/casopisy/ceska-slovenska-farmacie/2020-1-24/dvojite-obalene-pelety-se-semipermeabilnim-obalem-z-ethylcelulosy-urcene-k-detekci-inhibitoru-cholinesteras-122472},
volume = {69},
year = {2020}
}
TY - JOUR
ID - 1728040
AU - Zeman, Jiří - Pavloková, Sylvie - Vetchý, David - Pitschmann, Vladimír
PY - 2020
TI - Double-coated pellets with semipermeable ethylcellulose coating for detection of cholinesterase inhibitors
JF - Česká a slovenská farmacie
VL - 69
IS - 1
SP - 24-32
EP - 24-32
PB - Česká lékařská společnost J.E. Purkyně
SN - 12107816
KW - ethylcellulose
KW - pellets
KW - cholinesterase
KW - detection tube
KW - nerve agent
UR - http://www.prolekare.cz/casopisy/ceska-slovenska-farmacie/2020-1-24/dvojite-obalene-pelety-se-semipermeabilnim-obalem-z-ethylcelulosy-urcene-k-detekci-inhibitoru-cholinesteras-122472
N2 - Currently, nerve agents are often used in terrorist attacks or assassinations. In such cases, it is necessary to detect them quickly, accurately and easily right in the field. Detection tubes, which are small devices containing pellets with immobilized cholinesterase and detection reagents, meet these conditions. Their detection mechanism is based on a highly sensitive enzymatic Ellman reaction, when in the absence of cholinesterase inhibitors the pellets develop a visible yellow color, whereas in their presence the carriers retain the original color. The rate of reaction, its sensitivity and the distinct color transition are the key points of the research. In this experiment, double-coated pellets were prepared. The first coating contained the butyrylcholinesterase immobilized in hypromellose, while the second coating consisted of ethylcellulose and triethyl citrate. Based on the properties of such carriers, samples containing lactose dispersed in the ethylcellulose coating were also prepared, which was expected to have an effect on increasing the permeability of the coating and hence the detection rate and color intensity. In addition to selected physicochemical properties, carriers were evaluated for enzyme activity, sensitivity and color transition intensity. Samples showing the best properties were subjected to a 24-months stability test at three different temperatures and humidity.
ER -
ZEMAN, Jiří, Sylvie PAVLOKOVÁ, David VETCHÝ a Vladimír PITSCHMANN. Double-coated pellets with semipermeable ethylcellulose coating for detection of cholinesterase inhibitors. \textit{Česká a slovenská farmacie}. Česká lékařská společnost J.E. Purkyně, roč.~69, č.~1, s.~24-32. ISSN~1210-7816. 2020.
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