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@article{1728079,
author = {Nemcakova, Ivana and Blahová, Lucie and Rysanek, Petr and Blanquer, Andreu and Bacakova, Lucie and Zajíčková, Lenka},
article_location = {Basel},
article_number = {24},
doi = {http://dx.doi.org/10.3390/ijms21249467},
keywords = {amine plasma polymer; bioactive coating; polycaprolactone nanofibers; substrate morphology; cell adhesion; cell proliferation},
language = {eng},
issn = {1422-0067},
journal = {International Journal of Molecular Sciences},
note = {doc. Zajíčková - dle rozhodnutí ředitelů 5.3.2024},
title = {Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies},
url = {https://doi.org/10.3390/ijms21249467},
volume = {21},
year = {2020}
}
TY - JOUR
ID - 1728079
AU - Nemcakova, Ivana - Blahová, Lucie - Rysanek, Petr - Blanquer, Andreu - Bacakova, Lucie - Zajíčková, Lenka
PY - 2020
TI - Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies
JF - International Journal of Molecular Sciences
VL - 21
IS - 24
SP - 1-24
EP - 1-24
PB - MDPI
SN - 14220067
N1 - doc. Zajíčková - dle rozhodnutí ředitelů 5.3.2024
KW - amine plasma polymer
KW - bioactive coating
KW - polycaprolactone nanofibers
KW - substrate morphology
KW - cell adhesion
KW - cell proliferation
UR - https://doi.org/10.3390/ijms21249467
L2 - https://doi.org/10.3390/ijms21249467
N2 - Amine-coated biodegradable materials based on synthetic polymers have a great potential for tissue remodeling and regeneration because of their excellent processability and bioactivity. In the present study, we have investigated the influence of various chemical compositions of amine plasma polymer (PP) coatings and the influence of the substrate morphology, represented by polystyrene culture dishes and polycaprolactone nanofibers (PCL NFs), on the behavior of vascular smooth muscle cells (VSMCs). Although all amine-PP coatings improved the initial adhesion of VSMCs, 7-day long cultivation revealed a clear preference for the coating containing about 15 at.% of nitrogen (CPA-33). The CPA-33 coating demonstrated the ideal combination of good water stability, a sufficient amine group content, and favorable surface wettability and morphology. The nanostructured morphology of amine-PP-coated PCL NFs successfully slowed the proliferation rate of VSMCs, which is essential in preventing restenosis of vascular replacements in vivo. At the same time, CPA-33-coated PCL NFs supported the continuous proliferation of VSMCs during 7-day long cultivation, with no significant increase in cytokine secretion by RAW 264.7 macrophages. The CPA-33 coating deposited on biodegradable PCL NFs therefore seems to be a promising material for manufacturing small-diameter vascular grafts, which are still lacking on the current market.
ER -
NEMCAKOVA, Ivana, Lucie BLAHOVÁ, Petr RYSANEK, Andreu BLANQUER, Lucie BACAKOVA and Lenka ZAJÍČKOVÁ. Behaviour of Vascular Smooth Muscle Cells on Amine Plasma-Coated Materials with Various Chemical Structures and Morphologies. \textit{International Journal of Molecular Sciences}. Basel: MDPI, 2020, vol.~21, No~24, p.~1-24. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms21249467.
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