Other formats:
BibTeX
LaTeX
RIS
@article{1728092, author = {Hyzdalova, Martina and Procházková, Jiřina and Strapacova, Simona and Svrzkova, Lucie and Vacek, Ondřej and Fedr, Radek and Andrysík, Zdeněk and Hruba, Eva and Libalova, Helena and Klema, Jiri and Topinka, Jan and Masek, Josef and Souček, Karel and Vondráček, Jan and Machala, Miroslav}, article_location = {Oxford}, article_number = {January 2021}, doi = {http://dx.doi.org/10.1016/j.chemosphere.2020.128126}, keywords = {BaP; TCDD; Lung carcinoma; Cell proliferation; EMT; Tumor progression}, language = {eng}, issn = {0045-6535}, journal = {Chemosphere}, title = {A prolonged exposure of human lung carcinoma epithelial cells to benzo[a]pyrene induces p21-dependent epithelial-to-mesenchymal transition (EMT)-like phenotype}, url = {https://doi.org/10.1016/j.chemosphere.2020.128126}, volume = {263}, year = {2021} }
TY - JOUR ID - 1728092 AU - Hyzdalova, Martina - Procházková, Jiřina - Strapacova, Simona - Svrzkova, Lucie - Vacek, Ondřej - Fedr, Radek - Andrysík, Zdeněk - Hruba, Eva - Libalova, Helena - Klema, Jiri - Topinka, Jan - Masek, Josef - Souček, Karel - Vondráček, Jan - Machala, Miroslav PY - 2021 TI - A prolonged exposure of human lung carcinoma epithelial cells to benzo[a]pyrene induces p21-dependent epithelial-to-mesenchymal transition (EMT)-like phenotype JF - Chemosphere VL - 263 IS - January 2021 SP - 1-15 EP - 1-15 PB - Pergamon-Elsevier Science Ltd SN - 00456535 KW - BaP KW - TCDD KW - Lung carcinoma KW - Cell proliferation KW - EMT KW - Tumor progression UR - https://doi.org/10.1016/j.chemosphere.2020.128126 L2 - https://doi.org/10.1016/j.chemosphere.2020.128126 N2 - Deciphering the role of the aryl hydrocarbon receptor (AhR) in lung cancer cells may help us to better understand the role of toxic AhR ligands in lung carcinogenesis, including cancer progression. We employed human lung carcinoma A549 cells to investigate their fate after continuous two-week exposure to model AhR agonists, genotoxic benzo[a]pyrene (BaP; 1 mu M) and non-genotoxic 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD; 10 nM). While TCDD increased proliferative rate of A549 cells, exposure to BaP decreased cell proliferation and induced epithelial-to-mesenchymal transition (EMT)-like phenotype, which was associated with enhanced cell migration, invasion, and altered cell morphology. Although TCDD also suppressed expression of E-cadherin and activated some genes linked to EMT, it did not induce the EMT-like phenotype. The results of transcriptomic analysis, and the opposite effects of BaP and TCDD on cell proliferation, indicated that a delay in cell cycle progression, together with a slight increase of senescence (when coupled with AhR activation), favors the induction of EMT-like phenotype. The shift towards EMT-like phenotype observed after simultaneous treatment with TCDD and mitomycin C (an inhibitor of cell proliferation) confirmed the hypothesis. Since BaP decreased cell proliferative rate via induction of p21 expression, we generated the A549 cell model with reduced p21 expression and exposed it to BaP for two weeks. The p21 knockdown suppressed the BaP-mediated EMT-like phenotype in A549 cells, thus confirming that a delayed cell cycle progression, together with p21-dependent induction of senescence-related chemokine CCL2, may contribute to induction of EMT-like cell phenotype in lung cells exposed to genotoxic AhR ligands. ER -
HYZDALOVA, Martina, Jiřina PROCHÁZKOVÁ, Simona STRAPACOVA, Lucie SVRZKOVA, Ondřej VACEK, Radek FEDR, Zdeněk ANDRYSÍK, Eva HRUBA, Helena LIBALOVA, Jiri KLEMA, Jan TOPINKA, Josef MASEK, Karel SOUČEK, Jan VONDRÁČEK and Miroslav MACHALA. A prolonged exposure of human lung carcinoma epithelial cells to benzo[a]pyrene induces p21-dependent epithelial-to-mesenchymal transition (EMT)-like phenotype. \textit{Chemosphere}. Oxford: Pergamon-Elsevier Science Ltd, 2021, vol.~263, January 2021, p.~1-15. ISSN~0045-6535. Available from: https://dx.doi.org/10.1016/j.chemosphere.2020.128126.
|