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@article{1730444,
author = {Trebichalská, Zuzana and Kyjovská, D. and Kloudová, S. and Otevřel, P. and Hampl, Aleš and Holubcová, Zuzana},
article_location = {Cary},
article_number = {1},
doi = {http://dx.doi.org/10.1093/biolre/ioaa174},
keywords = {cytoplasmic maturation; human oocytes},
language = {eng},
issn = {0006-3363},
journal = {Biology of Reproduction},
title = {Cytoplasmic maturation in human oocytes: an ultrastructural study},
url = {https://academic.oup.com/biolreprod/article/104/1/106/5909494},
volume = {104},
year = {2021}
}
TY - JOUR
ID - 1730444
AU - Trebichalská, Zuzana - Kyjovská, D. - Kloudová, S. - Otevřel, P. - Hampl, Aleš - Holubcová, Zuzana
PY - 2021
TI - Cytoplasmic maturation in human oocytes: an ultrastructural study
JF - Biology of Reproduction
VL - 104
IS - 1
SP - 106-116
EP - 106-116
PB - Oxford University Press
SN - 00063363
KW - cytoplasmic maturation
KW - human oocytes
UR - https://academic.oup.com/biolreprod/article/104/1/106/5909494
L2 - https://academic.oup.com/biolreprod/article/104/1/106/5909494
N2 - Female fertility relies on successful egg development. Besides chromosome segregation, complex structural and biochemical changes in the cytoplasmic compartment are necessary to confer the female gamete the capacity to undergo normal fertilization and sustain embryonic development. Despite the profound impact on egg quality, morphological bases of cytoplasmic maturation remain largely unknown. Here, we report our findings from the ultrastructural analysis of 69 unfertilized human oocytes from 34 young and healthy egg donors. By comparison of samples fixed at three consecutive developmental stages, we explored how ooplasmic architecture changes during meiotic maturation in vitro. The morphometric image analysis supported observation that the major reorganization of cytoplasm occurs before polar body extrusion. The organelles initially concentrated around prophase nucleus were repositioned toward the periphery and evenly distributed throughout the ooplasm. As maturation progressed, distinct secretory apparatus appeared to transform into cortical granules that clustered underneath the oocyte's surface. The most prominent feature was the gradual formation of heterologous complexes composed of variable elements of endoplasmic reticulum and multiple mitochondria with primitive morphology. Based on the generated image dataset, we proposed a morphological map of cytoplasmic maturation, which may serve as a reference for future comparative studies. In conclusion, this work improves our understanding of human oocyte morphology, cytoplasmic maturation, and intracellular factors defining human egg quality. Although this analysis involved spare oocytes completing development in vitro, it provides essential insight into the enigmatic process by which human egg progenitors prepare for fertilization.
ER -
TREBICHALSKÁ, Zuzana, D. KYJOVSKÁ, S. KLOUDOVÁ, P. OTEVŘEL, Aleš HAMPL and Zuzana HOLUBCOVÁ. Cytoplasmic maturation in human oocytes: an ultrastructural study. \textit{Biology of Reproduction}. Cary: Oxford University Press, 2021, vol.~104, No~1, p.~106-116. ISSN~0006-3363. Available from: https://dx.doi.org/10.1093/biolre/ioaa174.
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