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@article{1730587,
author = {Forostyak, Serhij and Forostyak, O. and Kwok, J. C. F. and Romanyuk, N. and Rehorova, Monika and Kriska, Jan and Dayanithi, G. and RahaandChowdhury, R. and Jendelova, Pavla and Anderova, Miroslava and Fawcett, J. W. and Sykova, Eva},
article_location = {Basel},
article_number = {24},
doi = {http://dx.doi.org/10.3390/ijms21249593},
keywords = {proteoglycans; plasticity; neurodegeneration; stem cells; iPS; ALS; motoneuron death; transplantation},
language = {eng},
issn = {1422-0067},
journal = {International Journal of Molecular Sciences},
title = {Transplantation of Neural Precursors Derived from Induced Pluripotent Cells Preserve Perineuronal Nets and Stimulate Neural Plasticity in ALS Rats},
url = {https://www.mdpi.com/1422-0067/21/24/9593},
volume = {21},
year = {2020}
}
TY - JOUR
ID - 1730587
AU - Forostyak, Serhij - Forostyak, O. - Kwok, J. C. F. - Romanyuk, N. - Rehorova, Monika - Kriska, Jan - Dayanithi, G. - Raha-Chowdhury, R. - Jendelova, Pavla - Anderova, Miroslava - Fawcett, J. W. - Sykova, Eva
PY - 2020
TI - Transplantation of Neural Precursors Derived from Induced Pluripotent Cells Preserve Perineuronal Nets and Stimulate Neural Plasticity in ALS Rats
JF - International Journal of Molecular Sciences
VL - 21
IS - 24
SP - 1-25
EP - 1-25
PB - Multidisciplinary Digital Publishing Institute
SN - 14220067
KW - proteoglycans
KW - plasticity
KW - neurodegeneration
KW - stem cells
KW - iPS
KW - ALS
KW - motoneuron death
KW - transplantation
UR - https://www.mdpi.com/1422-0067/21/24/9593
L2 - https://www.mdpi.com/1422-0067/21/24/9593
N2 - A promising therapeutic strategy for amyotrophic lateral sclerosis (ALS) treatment is stem cell therapy. Neural progenitors derived from induced pluripotent cells (NP-iPS) might rescue or replace dying motoneurons (MNs). However, the mechanisms responsible for the beneficial effect are not fully understood. The aim here was to investigate the mechanism by studying the effect of intraspinally injected NP-iPS into asymptomatic and early symptomatic superoxide dismutase (SOD)1(G93A) transgenic rats. Prior to transplantation, NP-iPS were characterized in vitro for their ability to differentiate into a neuronal phenotype. Motor functions were tested in all animals, and the tissue was analyzed by immunohistochemistry, qPCR, and Western blot. NP-iPS transplantation significantly preserved MNs, slowed disease progression, and extended the survival of all treated animals. The dysregulation of spinal chondroitin sulfate proteoglycans was observed in SOD1(G93A) rats at the terminal stage. NP-iPS application led to normalized host genes expression (versican, has-1, tenascin-R, ngf, igf-1, bdnf, bax, bcl-2, and casp-3) and the protection of perineuronal nets around the preserved MNs. In the host spinal cord, transplanted cells remained as progenitors, many in contact with MNs, but they did not differentiate. The findings suggest that NP-iPS demonstrate neuroprotective properties by regulating local gene expression and regulate plasticity by modulating the central nervous system (CNS) extracellular matrix such as perineuronal nets (PNNs).
ER -
FOROSTYAK, Serhij, O. FOROSTYAK, J. C. F. KWOK, N. ROMANYUK, Monika REHOROVA, Jan KRISKA, G. DAYANITHI, R. RAHA-CHOWDHURY, Pavla JENDELOVA, Miroslava ANDEROVA, J. W. FAWCETT and Eva SYKOVA. Transplantation of Neural Precursors Derived from Induced Pluripotent Cells Preserve Perineuronal Nets and Stimulate Neural Plasticity in ALS Rats. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, 2020, vol.~21, No~24, p.~1-25. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms21249593.
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