Molecular Evolution and Diversification of Proteins Involved in
miRNA Maturation Pathway

J 2020

Molecular Evolution and Diversification of Proteins Involved in miRNA Maturation Pathway

MOTURU, Tarakaramji, Sansrity SINHA, Hymavathi SALAVA, Sravankumar THULA, Tomasz NODZYNSKI et. al.

Basic information

Original name

Molecular Evolution and Diversification of Proteins Involved in miRNA Maturation Pathway

Authors

MOTURU, Tarakaramji (356 India, guarantor, belonging to the institution), Sansrity SINHA, Hymavathi SALAVA, Sravankumar THULA (356 India, belonging to the institution), Tomasz NODZYNSKI (616 Poland, belonging to the institution), Radka SVOBODOVÁ (203 Czech Republic, belonging to the institution), Jiří FRIML (203 Czech Republic) and Sibu SIMON (356 India, belonging to the institution)

Edition

Plants, Basel, MDPI, 2020, 2223-7747

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.935

RIV identification code

RIV/00216224:14740/20:00114699

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.3390/plants9030299

UT WoS

000525315000035

Keywords in English

small RNA (smRNAs); Dawdle (DDL); Tough (TGH); Serrate (SE; ARS2); Argonaute (AGO); Dicer-Like (DCR; DCL); evolution; phylogeny

Abstract

V originále

Small RNAs (smRNA, 19-25 nucleotides long), which are transcribed by RNA polymerase II, regulate the expression of genes involved in a multitude of processes in eukaryotes. miRNA biogenesis and the proteins involved in the biogenesis pathway differ across plant and animal lineages. The major proteins constituting the biogenesis pathway, namely, the Dicers (DCL/DCR) and Argonautes (AGOs), have been extensively studied. However, the accessory proteins (DAWDLE (DDL), SERRATE (SE), and TOUGH (TGH)) of the pathway that differs across the two lineages remain largely uncharacterized. We present the first detailed report on the molecular evolution and divergence of these proteins across eukaryotes. Although DDL is present in eukaryotes and prokaryotes, SE and TGH appear to be specific to eukaryotes. The addition/deletion of specific domains and/or domain-specific sequence divergence in the three proteins points to the observed functional divergence of these proteins across the two lineages, which correlates with the differences in miRNA length across the two lineages. Our data enhance the current understanding of the structure-function relationship of these proteins and reveals previous unexplored crucial residues in the three proteins that can be used as a basis for further functional characterization. The data presented here on the number of miRNAs in crown eukaryotic lineages are consistent with the notion of the expansion of the number of miRNA-coding genes in animal and plant lineages correlating with organismal complexity. Whether this difference in functionally correlates with the diversification (or presence/absence) of the three proteins studied here or the miRNA signaling in the plant and animal lineages is unclear. Based on our results of the three proteins studied here and previously available data concerning the evolution of miRNA genes in the plant and animal lineages, we believe that miRNAs probably evolved once in the ancestor to crown eukaryotes and have diversified independently in the eukaryotes.

Links

GA13-40637S, research and development project

Name: Genetické studie k identifikaci molekulárních mechanizmů buněčné polarity a auxinového transportu v rostlinách

Investor: Czech Science Foundation

LM2010005, research and development project

Name: Velká infrastruktura CESNET (Acronym: VI CESNET)

Investor: Ministry of Education, Youth and Sports of the CR

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

MOTURU, Tarakaramji, Sansrity SINHA, Hymavathi SALAVA, Sravankumar THULA, Tomasz NODZYNSKI, Radka SVOBODOVÁ, Jiří FRIML and Sibu SIMON. Molecular Evolution and Diversification of Proteins Involved in miRNA Maturation Pathway. Plants. Basel: MDPI, 2020, vol. 9, No 3, p. 1-19. ISSN 2223-7747. Available from: https://dx.doi.org/10.3390/plants9030299.

@article{1730656,
   author = {Moturu, Tarakaramji and Sinha, Sansrity and Salava, Hymavathi and Thula, Sravankumar and Nodzynski, Tomasz and Svobodová, Radka and Friml, Jiří and Simon, Sibu},
   article_location = {Basel},
   article_number = {3},
   doi = {http://dx.doi.org/10.3390/plants9030299},
   keywords = {small RNA (smRNAs); Dawdle (DDL); Tough (TGH); Serrate (SE; ARS2); Argonaute (AGO); Dicer-Like (DCR; DCL); evolution; phylogeny},
   language = {eng},
   issn = {2223-7747},
   journal = {Plants},
   title = {Molecular Evolution and Diversification of Proteins Involved in miRNA Maturation Pathway},
   url = {https://doi.org/10.3390/plants9030299},
   volume = {9},
   year = {2020}
}

TY  - JOUR
ID  - 1730656
AU  - Moturu, Tarakaramji - Sinha, Sansrity - Salava, Hymavathi - Thula, Sravankumar - Nodzynski, Tomasz - Svobodová, Radka - Friml, Jiří - Simon, Sibu
PY  - 2020
TI  - Molecular Evolution and Diversification of Proteins Involved in miRNA Maturation Pathway
JF  - Plants
VL  - 9
IS  - 3
SP  - 1-19
EP  - 1-19
PB  - MDPI
SN  - 22237747
KW  - small RNA (smRNAs)
KW  - Dawdle (DDL)
KW  - Tough (TGH)
KW  - Serrate (SE
KW  - ARS2)
KW  - Argonaute (AGO)
KW  - Dicer-Like (DCR
KW  - DCL)
KW  - evolution
KW  - phylogeny
UR  - https://doi.org/10.3390/plants9030299
L2  - https://doi.org/10.3390/plants9030299
N2  - Small RNAs (smRNA, 19-25 nucleotides long), which are transcribed by RNA polymerase II, regulate the expression of genes involved in a multitude of processes in eukaryotes. miRNA biogenesis and the proteins involved in the biogenesis pathway differ across plant and animal lineages. The major proteins constituting the biogenesis pathway, namely, the Dicers (DCL/DCR) and Argonautes (AGOs), have been extensively studied. However, the accessory proteins (DAWDLE (DDL), SERRATE (SE), and TOUGH (TGH)) of the pathway that differs across the two lineages remain largely uncharacterized. We present the first detailed report on the molecular evolution and divergence of these proteins across eukaryotes. Although DDL is present in eukaryotes and prokaryotes, SE and TGH appear to be specific to eukaryotes. The addition/deletion of specific domains and/or domain-specific sequence divergence in the three proteins points to the observed functional divergence of these proteins across the two lineages, which correlates with the differences in miRNA length across the two lineages. Our data enhance the current understanding of the structure-function relationship of these proteins and reveals previous unexplored crucial residues in the three proteins that can be used as a basis for further functional characterization. The data presented here on the number of miRNAs in crown eukaryotic lineages are consistent with the notion of the expansion of the number of miRNA-coding genes in animal and plant lineages correlating with organismal complexity. Whether this difference in functionally correlates with the diversification (or presence/absence) of the three proteins studied here or the miRNA signaling in the plant and animal lineages is unclear. Based on our results of the three proteins studied here and previously available data concerning the evolution of miRNA genes in the plant and animal lineages, we believe that miRNAs probably evolved once in the ancestor to crown eukaryotes and have diversified independently in the eukaryotes.
ER  -

MOTURU, Tarakaramji, Sansrity SINHA, Hymavathi SALAVA, Sravankumar THULA, Tomasz NODZYNSKI, Radka SVOBODOVÁ, Jiří FRIML and Sibu SIMON. Molecular Evolution and Diversification of Proteins Involved in miRNA Maturation Pathway. \textit{Plants}. Basel: MDPI, 2020, vol.~9, No~3, p.~1-19. ISSN~2223-7747. Available from: https://dx.doi.org/10.3390/plants9030299.

Detailed Information on Publication Record