Study of synthetic oligonucleotides containing guanine by
circular dichroism spectroscopy and voltammetry on a pyrolytic
graphite electrode

D 2019

Study of synthetic oligonucleotides containing guanine by circular dichroism spectroscopy and voltammetry on a pyrolytic graphite electrode

OUTLÁ, Martina, Jan SPACEK, Daniel RENCIUK and Miroslav FOJTA

Basic information

Original name

Study of synthetic oligonucleotides containing guanine by circular dichroism spectroscopy and voltammetry on a pyrolytic graphite electrode

Authors

OUTLÁ, Martina (203 Czech Republic, belonging to the institution), Jan SPACEK, Daniel RENCIUK and Miroslav FOJTA (203 Czech Republic, belonging to the institution)

Edition

PRAGUE 2, PROCEEDINGS OF THE 15TH INTERNATIONAL STUDENTS CONFERENCE MODERN ANALYTICAL CHEMISTRY, p. 232-237, 6 pp. 2019

Publisher

CHARLES UNIV, FAC SCIENCE

Other information

Language

English

Type of outcome

Stať ve sborníku

Field of Study

10406 Analytical chemistry

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Publication form

printed version "print"

RIV identification code

RIV/00216224:14310/19:00117919

Organization unit

Faculty of Science

ISBN

978-80-7444-068-7

UT WoS

000576787700038

Keywords in English

circular dichroism spectroscopy; guanine; oxidation; voltammetry

Abstract

V originále

The possibility to study DNA quadruplex structures using voltammetric analysis of DNA hexamers was investigated using a pyrolytic graphite electrode in an acetate buffer (pH = 5.0). We have tested the influence of the hexamer sequence and DNA concentration their anodic oxidation. To verify the G quadruplex formation, or lack thereof, circular dichroism spectroscopy was used. Our results suggest that, on the pyrolytic graphite electrode, the guanine oxidation signals are not influenced by formation of G the quadruplex. Instead, the shape of the G oxidation multiple peak can be related to the primary structure of the oligonucleotide.

Citovat

OUTLÁ, Martina, Jan SPACEK, Daniel RENCIUK and Miroslav FOJTA. Study of synthetic oligonucleotides containing guanine by circular dichroism spectroscopy and voltammetry on a pyrolytic graphite electrode. In Nesmerak, K. PROCEEDINGS OF THE 15TH INTERNATIONAL STUDENTS CONFERENCE MODERN ANALYTICAL CHEMISTRY. PRAGUE 2: CHARLES UNIV, FAC SCIENCE, 2019, p. 232-237. ISBN 978-80-7444-068-7.

@inproceedings{1731057,
   author = {Outlá, Martina and Spacek, Jan and Renciuk, Daniel and Fojta, Miroslav},
   address = {PRAGUE 2},
   booktitle = {PROCEEDINGS OF THE 15TH INTERNATIONAL STUDENTS CONFERENCE MODERN ANALYTICAL CHEMISTRY},
   editor = {Nesmerak, K.},
   keywords = {circular dichroism spectroscopy; guanine; oxidation; voltammetry},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {PRAGUE 2},
   isbn = {978-80-7444-068-7},
   pages = {232-237},
   publisher = {CHARLES UNIV, FAC SCIENCE},
   title = {Study of synthetic oligonucleotides containing guanine by circular dichroism spectroscopy and voltammetry on a pyrolytic graphite electrode},
   year = {2019}
}

TY  - JOUR
ID  - 1731057
AU  - Outlá, Martina - Spacek, Jan - Renciuk, Daniel - Fojta, Miroslav
PY  - 2019
TI  - Study of synthetic oligonucleotides containing guanine by circular dichroism spectroscopy and voltammetry on a pyrolytic graphite electrode
PB  - CHARLES UNIV, FAC SCIENCE
CY  - PRAGUE 2
SN  - 9788074440687
KW  - circular dichroism spectroscopy
KW  - guanine
KW  - oxidation
KW  - voltammetry
N2  - The possibility to study DNA quadruplex structures using voltammetric analysis of DNA hexamers was investigated using a pyrolytic graphite electrode in an acetate buffer (pH = 5.0). We have tested the influence of the hexamer sequence and DNA concentration their anodic oxidation. To verify the G quadruplex formation, or lack thereof, circular dichroism spectroscopy was used. Our results suggest that, on the pyrolytic graphite electrode, the guanine oxidation signals are not influenced by formation of G the quadruplex. Instead, the shape of the G oxidation multiple peak can be related to the primary structure of the oligonucleotide.
ER  -

OUTLÁ, Martina, Jan SPACEK, Daniel RENCIUK and Miroslav FOJTA. Study of synthetic oligonucleotides containing guanine by circular dichroism spectroscopy and voltammetry on a pyrolytic graphite electrode. In Nesmerak, K. \textit{PROCEEDINGS OF THE 15TH INTERNATIONAL STUDENTS CONFERENCE MODERN ANALYTICAL CHEMISTRY}. PRAGUE 2: CHARLES UNIV, FAC SCIENCE, 2019, p.~232-237. ISBN~978-80-7444-068-7.

Detailed Information on Publication Record