| HORKÁ, Marie, Pavel KARÁSEK, Michal ROTH, Dana ŠTVERÁKOVÁ, Jiří ŠALPLACHTA, Filip RŮŽIČKA and Roman PANTŮČEK. Bacteriophage replication on permissive host cells in fused silica capillary with nanostructured part as potential of electrophoretic methods for developing phage applications. Talanta. Amsterdam: Elsevier, 2021, vol. 224, March 2021, p. "121800-1"-"121800-8", 8 pp. ISSN 0039-9140. Available from: https://dx.doi.org/10.1016/j.talanta.2020.121800. |
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@article{1734916,
author = {Horká, Marie and Karásek, Pavel and Roth, Michal and Štveráková, Dana and Šalplachta, Jiří and Růžička, Filip and Pantůček, Roman},
article_location = {Amsterdam},
article_number = {March 2021},
doi = {http://dx.doi.org/10.1016/j.talanta.2020.121800},
keywords = {Capillary electrophoretic methods; Fused silica capillary with roughened part; Microliter sample volumes; Dynamic adhesion of bacterial cells; Staphylococcus aureus; Bacteriophage propagation},
language = {eng},
issn = {0039-9140},
journal = {Talanta},
title = {Bacteriophage replication on permissive host cells in fused silica capillary with nanostructured part as potential of electrophoretic methods for developing phage applications},
url = {https://doi.org/10.1016/j.talanta.2020.121800},
volume = {224},
year = {2021}
}
TY - JOUR
ID - 1734916
AU - Horká, Marie - Karásek, Pavel - Roth, Michal - Štveráková, Dana - Šalplachta, Jiří - Růžička, Filip - Pantůček, Roman
PY - 2021
TI - Bacteriophage replication on permissive host cells in fused silica capillary with nanostructured part as potential of electrophoretic methods for developing phage applications
JF - Talanta
VL - 224
IS - March 2021
SP - "121800-1"-"121800-8"
EP - "121800-1"-"121800-8"
PB - Elsevier
SN - 00399140
KW - Capillary electrophoretic methods
KW - Fused silica capillary with roughened part
KW - Microliter sample volumes
KW - Dynamic adhesion of bacterial cells
KW - Staphylococcus aureus
KW - Bacteriophage propagation
UR - https://doi.org/10.1016/j.talanta.2020.121800
L2 - https://doi.org/10.1016/j.talanta.2020.121800
N2 - Phage therapy could offer a safe and effective alternative to antibiotic treatment of infections caused by Gram-positive bacterium Staphylococcus aureus that have emerged as a significant threat in hospital and community environment and is attracting growing interest among clinicians. The legislation process of approving the phage therapeutics by pharmaceutical authorities requires rapid analytical techniques for assessment of phage activity. Here, we present a three-step method for on-line monitoring the phage effect on bacterial cells dynamically adhered from microliter volumes of high conductivity matrix onto the inner surface of fused silica capillary with a part etched with supercritical water. Phage K1/420 particles of the Kayvirus genus generated by propagation on the host S. aureus cells together with the uninfected cells were concentrated, separated and detected using capillary electrophoretic methods. The phage interactions with selected S. aureus strains exhibiting differences in phage susceptibility were compared. The method allowed determination of the phage burst size and time of phage latent period in analyzed strains. Apart from enumeration of bacteriophages by the plaque assays, the proposed method is suitable for phage activity testing.
ER -
HORKÁ, Marie, Pavel KARÁSEK, Michal ROTH, Dana ŠTVERÁKOVÁ, Jiří ŠALPLACHTA, Filip RŮŽIČKA and Roman PANTŮČEK. Bacteriophage replication on permissive host cells in fused silica capillary with nanostructured part as potential of electrophoretic methods for developing phage applications. \textit{Talanta}. Amsterdam: Elsevier, 2021, vol.~224, March 2021, p.~''121800-1''-''121800-8'', 8 pp. ISSN~0039-9140. Available from: https://dx.doi.org/10.1016/j.talanta.2020.121800.
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