J 2021

Nucleolar rDNA folds into condensed foci with a specific combination of epigenetic marks

KUTASHEV, Konstantin, Michal FRANEK, Klev DIAMANTI, Jan KOMOROWSKI, Marie OLSINOVA et. al.

Basic information

Original name

Nucleolar rDNA folds into condensed foci with a specific combination of epigenetic marks

Authors

KUTASHEV, Konstantin (643 Russian Federation, belonging to the institution), Michal FRANEK (703 Slovakia, belonging to the institution), Klev DIAMANTI, Jan KOMOROWSKI, Marie OLSINOVA and Martina DVOŘÁČKOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Plant Journal, Hoboken, Wiley, 2021, 0960-7412

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 7.091

RIV identification code

RIV/00216224:14740/21:00118848

Organization unit

Central European Institute of Technology

DOI

UT WoS

000606381300001

Keywords in English

chromatin; rDNA; nucleolar foci; histone variants; chromatin immunoprecipitation; super-resolution microscopy

Tags

Tags

International impact, Reviewed
Změněno: 15/10/2024 14:18, Ing. Martina Blahová

Abstract

V originále

Arabidopsis thaliana 45S ribosomal genes (rDNA) are located in tandem arrays called nucleolus organizing regions on the termini of chromosomes 2 and 4 (NOR2 and NOR4) and encode rRNA, a crucial structural element of the ribosome. The current model of rDNA organization suggests that inactive rRNA genes accumulate in the condensed chromocenters in the nucleus and at the nucleolar periphery, while the nucleolus delineates active genes. We challenge the perspective that all intranucleolar rDNA is active by showing that a subset of nucleolar rDNA assembles into condensed foci marked by H3.1 and H3.3 histones that also contain the repressive H3K9me2 histone mark. By using plant lines containing a low number of rDNA copies, we further found that the condensed foci relate to the folding of rDNA, which appears to be a common mechanism of rDNA regulation inside the nucleolus. The H3K9me2 histone mark found in condensed foci represents a typical modification of bulk inactive rDNA, as we show by genome-wide approaches, similar to the H2A.W histone variant. The euchromatin histone marks H3K27me3 and H3K4me3, in contrast, do not colocalize with nucleolar foci and their overall levels in the nucleolus are very low. We further demonstrate that the rDNA promoter is an important regulatory region of the rDNA, where the distribution of histone variants and histone modifications are modulated in response to rDNA activity.

Links

EF16_013/0001775, research and development project
Name: Modernizace a podpora výzkumných aktivit národní infrastruktury pro biologické a medicínské zobrazování Czech-BioImaging
EF16_026/0008446, research and development project
Name: Integrace signálu a epigenetické reprogramování pro produktivitu rostlin
GJ19-11880Y, research and development project
Name: Chromatinové prostředí indukované genotoxickými látkami
Investor: Czech Science Foundation, Chromatin environment induced by genotoxic agents
LTC18048, research and development project
Name: Prostorové uspořádání významných jaderných domén a jejich proteinových složek
Investor: Ministry of Education, Youth and Sports of the CR, Spatial organisation of key nuclear domains and their protein components, INTER-COST
90129, large research infrastructures
Name: Czech-BioImaging II
90132, large research infrastructures
Name: NCMG II