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@article{1735418,
author = {Chermula, B. and Kranc, W. and Jopek, K. and BudnaandTukan, J. and Hutchings, G. and Dompe, C. and Moncrieff, L. and Janowicz, K. and Jozkowiak, M. and Ješeta, Michal and Petitte, J. and Mozdziak, P. and Pawelczyk, L. and Spaczynski, R. Z. and Kempisty, B.},
article_location = {ST ALBAN-ANLAGE},
article_number = {5},
doi = {http://dx.doi.org/10.3390/cells9051265},
keywords = {cumulus cells; human; programmed cell death; gene expression},
language = {eng},
issn = {2073-4409},
journal = {Cells},
title = {Human Cumulus Cells in Long-Term In Vitro Culture Reflect Differential Expression Profile of Genes Responsible for Planned Cell Death and Aging-A Study of New Molecular Markers},
url = {https://www.mdpi.com/2073-4409/9/5/1265},
volume = {9},
year = {2020}
}
TY - JOUR
ID - 1735418
AU - Chermula, B. - Kranc, W. - Jopek, K. - Budna-Tukan, J. - Hutchings, G. - Dompe, C. - Moncrieff, L. - Janowicz, K. - Jozkowiak, M. - Ješeta, Michal - Petitte, J. - Mozdziak, P. - Pawelczyk, L. - Spaczynski, R. Z. - Kempisty, B.
PY - 2020
TI - Human Cumulus Cells in Long-Term In Vitro Culture Reflect Differential Expression Profile of Genes Responsible for Planned Cell Death and Aging-A Study of New Molecular Markers
JF - Cells
VL - 9
IS - 5
SP - 1-21
EP - 1-21
PB - MDPI
SN - 20734409
KW - cumulus cells
KW - human
KW - programmed cell death
KW - gene expression
UR - https://www.mdpi.com/2073-4409/9/5/1265
L2 - https://www.mdpi.com/2073-4409/9/5/1265
N2 - In the ovarian follicle, maturation of the oocyte increases in the presence of somatic cells called cumulus cells (CCs). These cells form a direct barrier between the oocyte and external environment. Thanks to bidirectional communication, they have a direct impact on the oocyte, its quality and development potential. Understanding the genetic profile of CCs appears to be important in elucidating the physiology of oocytes. Long-term in vitro culture of CCs collected from patients undergoing controlled ovarian stimulation during in vitro fertilization procedure was conducted. Using microarray expression analysis, transcript levels were assessed on day 1, 7, 15, and 30 of culture. Apoptosis and aging of CCs strictly influence oocyte quality and subsequently the outcome of assisted reproductive technologies (ART). Thus, particular attention was paid to the analysis of genes involved in programmed cell death, aging, and apoptosis. Due to the detailed level of expression analysis of each of the 133 analyzed genes, three groups were selected: first with significantly decreased expression during the culture; second with the statistically lowest increase in expression; and third with the highest significant increase in expression. COL3A1, SFRP4, CTGF, HTR2B, VCAM1, TNFRSF11B genes, belonging to the third group, were identified as potential carriers of information on oocyte quality.
ER -
CHERMULA, B., W. KRANC, K. JOPEK, J. BUDNA-TUKAN, G. HUTCHINGS, C. DOMPE, L. MONCRIEFF, K. JANOWICZ, M. JOZKOWIAK, Michal JEŠETA, J. PETITTE, P. MOZDZIAK, L. PAWELCZYK, R. Z. SPACZYNSKI and B. KEMPISTY. Human Cumulus Cells in Long-Term In Vitro Culture Reflect Differential Expression Profile of Genes Responsible for Planned Cell Death and Aging-A Study of New Molecular Markers. \textit{Cells}. ST ALBAN-ANLAGE: MDPI, 2020, vol.~9, No~5, p.~1-21. ISSN~2073-4409. Available from: https://dx.doi.org/10.3390/cells9051265.
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