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@article{1739877,
author = {Holubcová, Zuzana and Otevrel, Pavel and Koudelka, Marek and Kloudova, Sona},
article_location = {NEW YORK},
article_number = {4},
doi = {http://dx.doi.org/10.1007/s10815-021-02070-y},
keywords = {IVF add-ons; Oocyte maturity; Polarized light microscopy; Theophylline; Testicular sperm},
language = {eng},
issn = {1058-0468},
journal = {Journal of Assisted Reproduction and Genetics},
title = {Live birth achieved despite the absence of ejaculated spermatozoa and mature oocytes retrieved: a case report},
url = {https://link.springer.com/article/10.1007/s10815-021-02070-y},
volume = {38},
year = {2021}
}
TY - JOUR
ID - 1739877
AU - Holubcová, Zuzana - Otevrel, Pavel - Koudelka, Marek - Kloudova, Sona
PY - 2021
TI - Live birth achieved despite the absence of ejaculated spermatozoa and mature oocytes retrieved: a case report
JF - Journal of Assisted Reproduction and Genetics
VL - 38
IS - 4
SP - 925-929
EP - 925-929
PB - SPRINGER/PLENUM PUBLISHERS
SN - 10580468
KW - IVF add-ons
KW - Oocyte maturity
KW - Polarized light microscopy
KW - Theophylline
KW - Testicular sperm
UR - https://link.springer.com/article/10.1007/s10815-021-02070-y
L2 - https://link.springer.com/article/10.1007/s10815-021-02070-y
N2 - The most common reason for in vitro fertilization (IVF) cycle cancelation is a lack of quality gametes available for intracytoplasmic sperm injection (ICSI). Here we present the successful fertility treatment of the couple affected by obstructive azoospermia combined with suboptimal response to controlled ovarian stimulation. Since the conventional approach appeared ineffective to overcome both partners| specific problems, the targeted interventions, namely, (1) pharmacological enhancement of sperm motility and (2) polarized light microscopy (PLM)-guided optimization of ICSI time, were applied to rescue the cycle with only immature oocytes and immotile testicular sperm retrieved. The treatment with theophylline aided the selection of viable spermatozoa derived from cryopreserved testicular tissue. When the traditional stimulation protocol failed to produce mature eggs, non-invasive spindle imaging was employed to adjust the sperm injection time to the maturational stage of oocytes extruding a polar body in vitro. The fertilization of 12 late-maturing oocytes yielded 5 zygotes, which all developed into blastocysts. One embryo was transferred into the uterus on day 5 post-fertilization, and another 3 good quality blastocysts were vitrified for later use. The pregnancy resulted in a full-term delivery of a healthy child. This case demonstrates that the individualization beyond the standard IVF protocols should be considered to maximize the chance of poor-prognosis patients to achieve pregnancy with their own gametes.
ER -
HOLUBCOVÁ, Zuzana, Pavel OTEVREL, Marek KOUDELKA and Sona KLOUDOVA. Live birth achieved despite the absence of ejaculated spermatozoa and mature oocytes retrieved: a case report. \textit{Journal of Assisted Reproduction and Genetics}. NEW YORK: SPRINGER/PLENUM PUBLISHERS, 2021, vol.~38, No~4, p.~925-929. ISSN~1058-0468. Available from: https://dx.doi.org/10.1007/s10815-021-02070-y.
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