Multiple In vitro biological effects of phenolic compounds from
Morus alba root bark

J 2020

Multiple In vitro biological effects of phenolic compounds from Morus alba root bark

ČULENOVÁ, Marie, Alice SYCHROVÁ, S. T. S. HASSAN, K. BERCHOVA-BIMOVA, P. SVOBODOVA et. al.

Basic information

Original name

Multiple In vitro biological effects of phenolic compounds from Morus alba root bark

Authors

ČULENOVÁ, Marie (203 Czech Republic, guarantor, belonging to the institution), Alice SYCHROVÁ (203 Czech Republic, belonging to the institution), S. T. S. HASSAN, K. BERCHOVA-BIMOVA, P. SVOBODOVA, A. HELCLOVA, H. MICHNOVA, J. HOSEK, H. VASILEV, Pavel SUCHÝ (203 Czech Republic, belonging to the institution), Gabriela KUZMÍNOVÁ (203 Czech Republic, belonging to the institution), Emil ŠVAJDLENKA (203 Czech Republic, belonging to the institution), Jan GAJDZIOK (203 Czech Republic, belonging to the institution), Alois ČÍŽEK (203 Czech Republic, belonging to the institution), Václav SUCHÝ (203 Czech Republic, belonging to the institution) and Karel ŠMEJKAL (203 Czech Republic, belonging to the institution)

Edition

JOURNAL OF ETHNOPHARMACOLOGY, CLARE, ELSEVIER IRELAND LTD, 2020, 0378-8741

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30104 Pharmacology and pharmacy

Country of publisher

Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.360

RIV identification code

RIV/00216224:14160/20:00118192

Organization unit

Faculty of Pharmacy

DOI

http://dx.doi.org/10.1016/j.jep.2019.112296

UT WoS

000503312800025

Keywords in English

Antiviral; Antibacterial; Anti-inflammatory; Docking; Mulberry; Phenolic

Abstract

V originále

Ethnopharmacological relevance: Morus alba L. is used in traditional Chinese medicine for the treatment of various diseases, including bacterial infections and inflammation. As a rich source of phenolic compounds, the plant is an object of many phytochemical and pharmacological studies. Aim of the study: The aim of the study was to isolate and evaluate possible parallel antiviral, antibacterial, and anti-inflammatory activities of phenolic mulberry compounds. Materials and methods: Extensive chromatographic separation of mulberry root bark extract and in vitro biological screening of 26 constituents identified promising candidates for further pharmacological research. Selected compounds were screened for anti-infective and anti-inflammatory activities. Antiviral activity was determined by the plaque number reduction assay and by the titer reduction assay, antibacterial using broth microdilution method, and anti-inflammatory activity using COX Colorimetric inhibitor screening assay kit. One compound was evaluated in vivo in carrageenan-induced paw-edema in mice. Results: Five prenylated compounds 1, 2, 8, 9, and 11, together with a simple phenolic ester 13, exhibited inhibitory activity against the replication of herpes simplex virus 1 (HSV-1) or herpes simplex virus 2 (HSV-2), with IC50 values ranging from 0.64 to 1.93 mu g/mL, and EC50 values 0.93 and 1.61 mu g/mL. Molecular docking studies demonstrated the effects of the active compounds by targeting HSV-1 DNA polymerase and HSV-2 protease. In antibacterial assay, compounds 1, 4, 11, and 17 diminished the growth of all of the Gram-positive strains tested, with MIC values of 1-16 mu g/mL. The anti-inflammatory ability of several compounds to inhibit cyclooxygenase 2 (COX-2) was tested in vitro, and compound 16 displayed greater activity than the indomethacin, positive control. Mulberrofuran B (11) showed anti-inflammatory activity in vivo against carrageenan-induced paw-edema in mice. Conclusions: Experimental investigation showed promising antiviral, antibacterial, and/or anti-inflammatory activities of the phenolic mulberry constituents, often with multiple inhibitory effects that might be used as a potential source of new medicine.

Citovat

ČULENOVÁ, Marie, Alice SYCHROVÁ, S. T. S. HASSAN, K. BERCHOVA-BIMOVA, P. SVOBODOVA, A. HELCLOVA, H. MICHNOVA, J. HOSEK, H. VASILEV, Pavel SUCHÝ, Gabriela KUZMÍNOVÁ, Emil ŠVAJDLENKA, Jan GAJDZIOK, Alois ČÍŽEK, Václav SUCHÝ and Karel ŠMEJKAL. Multiple In vitro biological effects of phenolic compounds from Morus alba root bark. JOURNAL OF ETHNOPHARMACOLOGY. CLARE: ELSEVIER IRELAND LTD, 2020, vol. 248, No 112296, p. 1-12. ISSN 0378-8741. Available from: https://dx.doi.org/10.1016/j.jep.2019.112296.

@article{1743498,
   author = {Čulenová, Marie and Sychrová, Alice and Hassan, S. T. S. and BerchovaandBimova, K. and Svobodova, P. and Helclova, A. and Michnova, H. and Hosek, J. and Vasilev, H. and Suchý, Pavel and Kuzmínová, Gabriela and Švajdlenka, Emil and Gajdziok, Jan and Čížek, Alois and Suchý, Václav and Šmejkal, Karel},
   article_location = {CLARE},
   article_number = {112296},
   doi = {http://dx.doi.org/10.1016/j.jep.2019.112296},
   keywords = {Antiviral; Antibacterial; Anti-inflammatory; Docking; Mulberry; Phenolic},
   language = {eng},
   issn = {0378-8741},
   journal = {JOURNAL OF ETHNOPHARMACOLOGY},
   title = {Multiple In vitro biological effects of phenolic compounds from Morus alba root bark},
   url = {https://www.sciencedirect.com/science/article/pii/S0378874119337298?via%3Dihub},
   volume = {248},
   year = {2020}
}

TY  - JOUR
ID  - 1743498
AU  - Čulenová, Marie - Sychrová, Alice - Hassan, S. T. S. - Berchova-Bimova, K. - Svobodova, P. - Helclova, A. - Michnova, H. - Hosek, J. - Vasilev, H. - Suchý, Pavel - Kuzmínová, Gabriela - Švajdlenka, Emil - Gajdziok, Jan - Čížek, Alois - Suchý, Václav - Šmejkal, Karel
PY  - 2020
TI  - Multiple In vitro biological effects of phenolic compounds from Morus alba root bark
JF  - JOURNAL OF ETHNOPHARMACOLOGY
VL  - 248
IS  - 112296
SP  - 1-12
EP  - 1-12
PB  - ELSEVIER IRELAND LTD
SN  - 03788741
KW  - Antiviral
KW  - Antibacterial
KW  - Anti-inflammatory
KW  - Docking
KW  - Mulberry
KW  - Phenolic
UR  - https://www.sciencedirect.com/science/article/pii/S0378874119337298?via%3Dihub
L2  - https://www.sciencedirect.com/science/article/pii/S0378874119337298?via%3Dihub
N2  - Ethnopharmacological relevance: Morus alba L. is used in traditional Chinese medicine for the treatment of various diseases, including bacterial infections and inflammation. As a rich source of phenolic compounds, the plant is an object of many phytochemical and pharmacological studies. Aim of the study: The aim of the study was to isolate and evaluate possible parallel antiviral, antibacterial, and anti-inflammatory activities of phenolic mulberry compounds. Materials and methods: Extensive chromatographic separation of mulberry root bark extract and in vitro biological screening of 26 constituents identified promising candidates for further pharmacological research. Selected compounds were screened for anti-infective and anti-inflammatory activities. Antiviral activity was determined by the plaque number reduction assay and by the titer reduction assay, antibacterial using broth microdilution method, and anti-inflammatory activity using COX Colorimetric inhibitor screening assay kit. One compound was evaluated in vivo in carrageenan-induced paw-edema in mice. Results: Five prenylated compounds 1, 2, 8, 9, and 11, together with a simple phenolic ester 13, exhibited inhibitory activity against the replication of herpes simplex virus 1 (HSV-1) or herpes simplex virus 2 (HSV-2), with IC50 values ranging from 0.64 to 1.93 mu g/mL, and EC50 values 0.93 and 1.61 mu g/mL. Molecular docking studies demonstrated the effects of the active compounds by targeting HSV-1 DNA polymerase and HSV-2 protease. In antibacterial assay, compounds 1, 4, 11, and 17 diminished the growth of all of the Gram-positive strains tested, with MIC values of 1-16 mu g/mL. The anti-inflammatory ability of several compounds to inhibit cyclooxygenase 2 (COX-2) was tested in vitro, and compound 16 displayed greater activity than the indomethacin, positive control. Mulberrofuran B (11) showed anti-inflammatory activity in vivo against carrageenan-induced paw-edema in mice. Conclusions: Experimental investigation showed promising antiviral, antibacterial, and/or anti-inflammatory activities of the phenolic mulberry constituents, often with multiple inhibitory effects that might be used as a potential source of new medicine.
ER  -

ČULENOVÁ, Marie, Alice SYCHROVÁ, S. T. S. HASSAN, K. BERCHOVA-BIMOVA, P. SVOBODOVA, A. HELCLOVA, H. MICHNOVA, J. HOSEK, H. VASILEV, Pavel SUCHÝ, Gabriela KUZMÍNOVÁ, Emil ŠVAJDLENKA, Jan GAJDZIOK, Alois ČÍŽEK, Václav SUCHÝ and Karel ŠMEJKAL. Multiple In vitro biological effects of phenolic compounds from Morus alba root bark. \textit{JOURNAL OF ETHNOPHARMACOLOGY}. CLARE: ELSEVIER IRELAND LTD, 2020, vol.~248, No~112296, p.~1-12. ISSN~0378-8741. Available from: https://dx.doi.org/10.1016/j.jep.2019.112296.

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