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@article{1744860,
author = {Gabło, Natalia Anna and Trachtová, Karolína and Procházka, Vladimír and Hlavsa, Jan and Grolich, Tomáš and Kiss, Igor and Srovnal, Josef and Rehulkova, Alona and Lovecek, Martin and Skalicky, Pavel and BerindanandNeagoe, Ioana and Kala, Zdeněk and Slabý, Ondřej},
article_location = {BASEL},
article_number = {8},
doi = {http://dx.doi.org/10.3390/jcm9082440},
keywords = {Pancreatic ductal adenocarcinoma; prognosis; microRNAs},
language = {eng},
issn = {2077-0383},
journal = {Journal of Clinical Medicine},
title = {Identification and Validation of Circulating Micrornas as Prognostic Biomarkers in Pancreatic Ductal Adenocarcinoma Patients Undergoing Surgical Resection},
url = {https://www.mdpi.com/2077-0383/9/8/2440},
volume = {9},
year = {2020}
}
TY - JOUR
ID - 1744860
AU - Gabło, Natalia Anna - Trachtová, Karolína - Procházka, Vladimír - Hlavsa, Jan - Grolich, Tomáš - Kiss, Igor - Srovnal, Josef - Rehulkova, Alona - Lovecek, Martin - Skalicky, Pavel - Berindan-Neagoe, Ioana - Kala, Zdeněk - Slabý, Ondřej
PY - 2020
TI - Identification and Validation of Circulating Micrornas as Prognostic Biomarkers in Pancreatic Ductal Adenocarcinoma Patients Undergoing Surgical Resection
JF - Journal of Clinical Medicine
VL - 9
IS - 8
SP - 1-12
EP - 1-12
PB - MDPI
SN - 20770383
KW - Pancreatic ductal adenocarcinoma
KW - prognosis
KW - microRNAs
UR - https://www.mdpi.com/2077-0383/9/8/2440
N2 - Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal and aggressive cancers with a less than 6% five-year survival rate. Circulating microRNAs (miRNAs) are emerging as a useful tool for non-invasive diagnosis and prognosis estimation in the various cancer types, including PDAC. Our study aimed to evaluate whether miRNAs in the pre-operative blood plasma specimen have the potential to predict the prognosis of PDAC patients. In total, 112 PDAC patients planned for surgical resection were enrolled in our prospective study. To identify prognostic miRNAs, we used small RNA sequencing in 24 plasma samples of PDAC patients with poor prognosis (overall survival (OS) < 16 months) and 24 plasma samples of PDAC patients with a good prognosis (OS > 20 months). qPCR validation of selected miRNA candidates was performed in the independent cohort of PDAC patients (n= 64). In the discovery phase of the study, we identified 44 miRNAs with significantly different levels in the plasma samples of the group of good and poor prognosis patients. Among these miRNAs, 23 showed lower levels, and 21 showed higher levels in plasma specimens from PDAC patients with poor prognosis. Eleven miRNAs were selected for the validation, but only miR-99a-5p and miR-365a-3p were confirmed to have significantly lower levels and miR-200c-3p higher levels in plasma samples of poor prognosis cases. Using the combination of these 3-miRNA levels, we were able to identify the patients with poor prognosis with sensitivity 85% and specificity 80% (Area Under the Curve = 0.890). Overall, 3-miRNA prognostic score associated with OS was identified in the pre-operative blood plasma samples of PDAC patients undergoing surgical resection. Following further independent validations, the detection of these miRNA may enable identification of PDAC patients who have no survival benefit from the surgical treatment, which is associated with the high morbidity rates.
ER -
GABŁO, Natalia Anna, Karolína TRACHTOVÁ, Vladimír PROCHÁZKA, Jan HLAVSA, Tomáš GROLICH, Igor KISS, Josef SROVNAL, Alona REHULKOVA, Martin LOVECEK, Pavel SKALICKY, Ioana BERINDAN-NEAGOE, Zdeněk KALA a Ondřej SLABÝ. Identification and Validation of Circulating Micrornas as Prognostic Biomarkers in Pancreatic Ductal Adenocarcinoma Patients Undergoing Surgical Resection. \textit{Journal of Clinical Medicine}. BASEL: MDPI, roč.~9, č.~8, s.~1-12. ISSN~2077-0383. doi:10.3390/jcm9082440. 2020.
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