Transcriptomic and Proteomic Insights into Amborella trichopoda
Male Gametophyte Functions

J 2020

Transcriptomic and Proteomic Insights into Amborella trichopoda Male Gametophyte Functions

FLORES-TORNERO, Maria, Frank VOGLER, Marek MUTWIL, David POTĚŠIL, Ivana IHNATOVÁ et. al.

Basic information

Original name

Transcriptomic and Proteomic Insights into Amborella trichopoda Male Gametophyte Functions

Authors

FLORES-TORNERO, Maria (276 Germany), Frank VOGLER (276 Germany), Marek MUTWIL (276 Germany), David POTĚŠIL (203 Czech Republic, belonging to the institution), Ivana IHNATOVÁ (703 Slovakia, belonging to the institution), Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution), Stefanie SPRUNCK (276 Germany) and Thomas DRESSELHAUS (276 Germany)

Edition

Plant Physiology, American Society of Plant Physiologists, 2020, 0032-0889

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

fulltext

Impact factor

Impact factor: 8.340

RIV identification code

RIV/00216224:14740/20:00118232

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1104/pp.20.00837

UT WoS

000601303200010

Keywords in English

POLLEN-TUBE GROWTH; PECTIN METHYLESTERASE; MATURE POLLEN; SPERM CELLS; GERMINATED POLLEN; VEGETATIVE NUCLEI; GENERATIVE CELLS; TOMATO POLLEN; UDP-GLUCOSE; MOTHER CELL

Abstract

V originále

Transcriptomic and proteomic data from the basal angiosperm Amborella provide a resource to understand the evolution of male gametophyte functions in flowering plants. Flowering plants (angiosperms) are characterized by pollen tubes (PTs; male gametophytes) carrying two immobile sperm cells that grow over long distances through the carpel toward the ovules, where double fertilization is executed. It is not understood how these reproductive structures evolved, which genes occur de novo in male gametophytes of angiosperms, and to which extent PT functions are conserved among angiosperms. To contribute to a deeper understanding of the evolution of gametophyte functions, we generated RNA sequencing data from seven reproductive and two vegetative control tissues of the basal angiosperm Amborella trichopoda and complemented these with proteomic data of pollen grains (PGs) and PTs. The eudicot model plant Arabidopsis (Arabidopsis thaliana) served as a reference organism for data analysis, as more than 200 genes have been associated with male gametophyte functions in this species. We describe methods to collect bicellular A. trichopoda PGs, to induce their germination in vitro, and to monitor PT growth and germ cell division. Transcriptomic and proteomic analyses indicate that A. trichopoda PGs are prepared for germination requiring lipids, energy, but likely also reactive oxygen species, while PTs are especially characterized by catabolic/biosynthetic and transport processes including cell wall biosynthesis and gene regulation. Notably, a number of pollen-specific genes were lacking in Arabidopsis, and the number of genes involved in pollen signaling is significantly reduced in A. trichopoda. In conclusion, we provide insight into male gametophyte functions of the most basal angiosperm and establish a valuable resource for future studies on the evolution of flowering plants.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

90127, large research infrastructures

Name: CIISB II

Citovat

FLORES-TORNERO, Maria, Frank VOGLER, Marek MUTWIL, David POTĚŠIL, Ivana IHNATOVÁ, Zbyněk ZDRÁHAL, Stefanie SPRUNCK and Thomas DRESSELHAUS. Transcriptomic and Proteomic Insights into Amborella trichopoda Male Gametophyte Functions. Plant Physiology. American Society of Plant Physiologists, 2020, vol. 184, No 4, p. 1640-1657. ISSN 0032-0889. Available from: https://dx.doi.org/10.1104/pp.20.00837.

@article{1745061,
   author = {FloresandTornero, Maria and Vogler, Frank and Mutwil, Marek and Potěšil, David and Ihnatová, Ivana and Zdráhal, Zbyněk and Sprunck, Stefanie and Dresselhaus, Thomas},
   article_number = {4},
   doi = {http://dx.doi.org/10.1104/pp.20.00837},
   keywords = {POLLEN-TUBE GROWTH; PECTIN METHYLESTERASE; MATURE POLLEN; SPERM CELLS; GERMINATED POLLEN; VEGETATIVE NUCLEI; GENERATIVE CELLS; TOMATO POLLEN; UDP-GLUCOSE; MOTHER CELL},
   language = {eng},
   issn = {0032-0889},
   journal = {Plant Physiology},
   title = {Transcriptomic and Proteomic Insights into Amborella trichopoda Male Gametophyte Functions},
   url = {http://www.plantphysiol.org/content/plantphysiol/184/4/1640.full.pdf},
   volume = {184},
   year = {2020}
}

TY  - JOUR
ID  - 1745061
AU  - Flores-Tornero, Maria - Vogler, Frank - Mutwil, Marek - Potěšil, David - Ihnatová, Ivana - Zdráhal, Zbyněk - Sprunck, Stefanie - Dresselhaus, Thomas
PY  - 2020
TI  - Transcriptomic and Proteomic Insights into Amborella trichopoda Male Gametophyte Functions
JF  - Plant Physiology
VL  - 184
IS  - 4
SP  - 1640-1657
EP  - 1640-1657
PB  - American Society of Plant Physiologists
SN  - 00320889
KW  - POLLEN-TUBE GROWTH
KW  - PECTIN METHYLESTERASE
KW  - MATURE POLLEN
KW  - SPERM CELLS
KW  - GERMINATED POLLEN
KW  - VEGETATIVE NUCLEI
KW  - GENERATIVE CELLS
KW  - TOMATO POLLEN
KW  - UDP-GLUCOSE
KW  - MOTHER CELL
UR  - http://www.plantphysiol.org/content/plantphysiol/184/4/1640.full.pdf
N2  - Transcriptomic and proteomic data from the basal angiosperm Amborella provide a resource to understand the evolution of male gametophyte functions in flowering plants. Flowering plants (angiosperms) are characterized by pollen tubes (PTs; male gametophytes) carrying two immobile sperm cells that grow over long distances through the carpel toward the ovules, where double fertilization is executed. It is not understood how these reproductive structures evolved, which genes occur de novo in male gametophytes of angiosperms, and to which extent PT functions are conserved among angiosperms. To contribute to a deeper understanding of the evolution of gametophyte functions, we generated RNA sequencing data from seven reproductive and two vegetative control tissues of the basal angiosperm Amborella trichopoda and complemented these with proteomic data of pollen grains (PGs) and PTs. The eudicot model plant Arabidopsis (Arabidopsis thaliana) served as a reference organism for data analysis, as more than 200 genes have been associated with male gametophyte functions in this species. We describe methods to collect bicellular A. trichopoda PGs, to induce their germination in vitro, and to monitor PT growth and germ cell division. Transcriptomic and proteomic analyses indicate that A. trichopoda PGs are prepared for germination requiring lipids, energy, but likely also reactive oxygen species, while PTs are especially characterized by catabolic/biosynthetic and transport processes including cell wall biosynthesis and gene regulation. Notably, a number of pollen-specific genes were lacking in Arabidopsis, and the number of genes involved in pollen signaling is significantly reduced in A. trichopoda. In conclusion, we provide insight into male gametophyte functions of the most basal angiosperm and establish a valuable resource for future studies on the evolution of flowering plants.
ER  -

FLORES-TORNERO, Maria, Frank VOGLER, Marek MUTWIL, David POTĚŠIL, Ivana IHNATOVÁ, Zbyněk ZDRÁHAL, Stefanie SPRUNCK and Thomas DRESSELHAUS. Transcriptomic and Proteomic Insights into Amborella trichopoda Male Gametophyte Functions. \textit{Plant Physiology}. American Society of Plant Physiologists, 2020, vol.~184, No~4, p.~1640-1657. ISSN~0032-0889. Available from: https://dx.doi.org/10.1104/pp.20.00837.

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